Potential application of menadione for antimicrobial coating of surgical sutures

Yap, Cheng Hong; Lim, See Khai; Chan, Yun Li; Chee, Chin Fei; Tay, Sun Tee

doi:10.1016/j.biotno.2023.02.001

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…MENA exhibited a bacteriostatic effect in all tested isolates, which aligns with the findings of Yap et al . [100] with MRSA and MSSA strains. Other naphthoquinone derivatives have also been observed to exhibit bacteriostatic activity [101, 102].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of menadione alone and in combination with oxacillin against methicillin-resistant Staphylococcus aureus and its impact on biofilms

Leitão,

Ferreira,

Gurgel do Amaral Valente Sá

et al. 2023

Journal of Medical Microbiology

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Introduction. Antibiotic resistance is a major threat to public health, particularly with methicillin-resistant Staphylococcus aureus (MRSA) being a leading cause of antimicrobial resistance. To combat this problem, drug repurposing offers a promising solution for the discovery of new antibacterial agents. Hypothesis. Menadione exhibits antibacterial activity against methicillin-sensitive and methicillin-resistant S. aureus strains, both alone and in combination with oxacillin. Its primary mechanism of action involves inducing oxidative stress. Methodology. Sensitivity assays were performed using broth microdilution. The interaction between menadione, oxacillin, and antioxidants was assessed using checkerboard technique. Mechanism of action was evaluated using flow cytometry, fluorescence microscopy, and in silico analysis. Aim. The aim of this study was to evaluate the in vitro antibacterial potential of menadione against planktonic and biofilm forms of methicillin-sensitive and resistant S. aureus strains. It also examined its role as a modulator of oxacillin activity and investigated the mechanism of action involved in its activity. Results. Menadione showed antibacterial activity against planktonic cells at concentrations ranging from 2 to 32 µg ml−1, with bacteriostatic action. When combined with oxacillin, it exhibited an additive and synergistic effect against the tested strains. Menadione also demonstrated antibiofilm activity at subinhibitory concentrations and effectively combated biofilms with reduced sensitivity to oxacillin alone. Its mechanism of action involves the production of reactive oxygen species (ROS) and DNA damage. It also showed interactions with important targets, such as DNA gyrase and dehydroesqualene synthase. The presence of ascorbic acid reversed its effects. Conclusion. Menadione exhibited antibacterial and antibiofilm activity against MRSA strains, suggesting its potential as an adjunct in the treatment of S. aureus infections. The main mechanism of action involves the production of ROS, which subsequently leads to DNA damage. Additionally, the activity of menadione can be complemented by its interaction with important virulence targets.

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Section: Discussionmentioning

confidence: 99%

Antibacterial activity of menadione alone and in combination with oxacillin against methicillin-resistant Staphylococcus aureus and its impact on biofilms

Leitão,

Ferreira,

Gurgel do Amaral Valente Sá

et al. 2023

Journal of Medical Microbiology

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“…The application of vitamins in treating MDR biofilms is receiving increased amount of attention. For instance, vitamin K3 (menadione)-coated sutures have been shown to be effective as powerful biofilm inhibitors against staphylococcal-associated surgical site infections [ 14 ]. In addition, ascorbic acid (vitamin C) has shown promising activity as an antibacterial and antibiofilm agent against carbapenem-resistant hypervirulent K. pneumoniae, P. aeruginosa, Proteus mirabilis and uropathogenic E. coli [ 15 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

Vitamin D and vitamin K1 as novel inhibitors of biofilm in Gram-negative bacteria

Lutfi,

Shaaban,

Elshaer

2024

BMC Microbiol

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Background The persistent surge in antimicrobial resistance represents a global disaster. The initial attachment and maturation of microbial biofilms are intimately related to antimicrobial resistance, which in turn exacerbates the challenge of eradicating bacterial infections. Consequently, there is a pressing need for novel therapies to be employed either independently or as adjuvants to diminish bacterial virulence and pathogenicity. In this context, we propose a novel approach focusing on vitamin D and vitamin K1 as potential antibiofilm agents that target Gram-negative bacteria which are hazardous to human health. Results Out of 130 Gram-negative bacterial isolates, 117 were confirmed to be A. baumannii (21 isolates, 17.9%), K. pneumoniae (40 isolates, 34.2%) and P. aeruginosa (56 isolates, 47.9%). The majority of the isolates were obtained from blood and wound specimens (27.4% each). Most of the isolates exhibited high resistance rates to β-lactams (60.7–100%), ciprofloxacin (62.5–100%), amikacin (53.6–76.2%) and gentamicin (65-71.4%). Approximately 93.2% of the isolates were biofilm producers, with 6.8% categorized as weak, 42.7% as moderate, and 50.4% as strong biofilm producers. The minimum inhibitory concentrations (MICs) of vitamin D and vitamin K1 were 625–1250 µg mL-1 and 2500–5000 µg mL-1, respectively, against A. baumannii (A5, A20 and A21), K. pneumoniae (K25, K27 and K28), and P. aeruginosa (P8, P16, P24 and P27) clinical isolates and standard strains A. baumannii (ATCC 19606 and ATCC 17978), K. pneumoniae (ATCC 51503) and P. aeruginosa PAO1 and PAO14. Both vitamins significantly decreased bacterial attachment and significantly eradicated mature biofilms developed by the selected standard and clinical Gram-negative isolates. The anti-biofilm effects of both supplements were confirmed by a notable decrease in the relative expression of the biofilm-encoding genes cusD, bssS and pelA in A. baumannii A5, K. pneumoniae K28 and P. aeruginosa P16, respectively. Conclusion This study highlights the anti-biofilm activity of vitamins D and K1 against the tested Gram-negative strains, which emphasizes the potential of these vitamins for use as adjuvant therapies to increase the efficacy of treatment for infections caused by multidrug-resistant (MDR) strains and biofilm-forming phenotypes. However, further validation through in vivo studies is needed to confirm these promising results.

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Antimicrobial nanolayer films of chloroxylenol–carboxyethylchitosan–modified silver nanoparticles for enhanced surgical suture performance

Chittratan,

Detsri,

Chalitangkoon

et al. 2024

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Potential application of menadione for antimicrobial coating of surgical sutures

Cited by 3 publications

References 32 publications

Antibacterial activity of menadione alone and in combination with oxacillin against methicillin-resistant Staphylococcus aureus and its impact on biofilms

Antibacterial activity of menadione alone and in combination with oxacillin against methicillin-resistant Staphylococcus aureus and its impact on biofilms

Vitamin D and vitamin K1 as novel inhibitors of biofilm in Gram-negative bacteria

Antimicrobial nanolayer films of chloroxylenol–carboxyethylchitosan–modified silver nanoparticles for enhanced surgical suture performance

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