Potential application of Pseudomonas stutzeri W228 for removal of copper and lead from marine environments

Abstract

High concentrations of metals in the environment alter bacterial diversity, selecting resistant and tolerant species. The study evaluated the selection of a potential bacterial strain from Sepetiba Bay-Rio de Janeiro, Brazil marine sediments to remove Cu and Pb. The bacterial strain isolated from the sediments was used in three different bioassays: (1) Cu at concentrations of 0 (control), 6 and 50 μg.mL -1 ; (2) Pb at concentrations of 0 (control), 6 and 50 μg.mL -1 ; (3) Cu + Pb in concentrations of 3 μg.mL -1 Cu + 3 μg.mL -1 Pb (6 μg.mL -1 ) and 25 μg.mL -1 Cu + 25 μg.mL -1 Pb (50 μg.mL -1 ). The number of cells and the enzymatic activities of dehydrogenases and esterases were quantified. Results of taxonomic identification indicated the selection of the Pseudomonas stutzeri W228 strain, showing a greater degree of similarity (±73%) with the database used. There was no significant variation in the number of cells, 10 8 cells.mL -1 , which represents a high biomass production in the presence of stressors. However, we observed a reduction in dehydrogenase activity at all tested concentrations of Cu, Pb and Cu + Pb. The activity of esterase increased, indicating a higher energy demand to complete the bacterial life cycle. The study showed significant results for the absorption of Pb by the extracellular polymeric substances (EPS) and the efflux of Cu. The capacity of Pb absorption by EPS can be considered a resistance mechanism, as well as the efflux of Cu, so that the available EPS sites could be occupied by the most toxic ions demonstrating that Pseudomonas stutzeri is resistant to Pb and Cu.

Developing a biocatalyst showcasing the synergistic effect of rice husk biochar and bacterial cells for the removal of heavy metals

“…This aligns with the results of Coelho da Costa Waite et al who also reported a maximum temperature of 37 °C for P. stutzeri growth in a study on Cu and Pb removal. 63 The percentage of Ni removal increased from 18 to 43.6% with an increase in temperature from 25 to 37 °C by employing free cells. PRHB showed a 47.82% Ni removal capacity at 25 °C, which increased to 67.52% at 37 °C.…”

“…This metal ion-resistant bacterium demonstrated resistance to mercury and chromium in our preliminary microbial tolerance study, suggesting its potential for removing these metal ions. Furthermore, studies by Coelho da Costa Waite et al employed P. stutzeri strain W228 to target Cu and Pb removal, 63 while Sathish Kumar et al utilized P. stutzeri L1, 67 and Kumari et al employed P. stutzeri Cr8 for chromium removal. 68 The immobilization of cells on PRHB resulted in a substantial improvement in the removal of both Cd and Ni.…”

Bioremediation potential of consortium Pseudomonas Stutzeri LBR and Cupriavidus Metallidurans LBJ in soil polluted by lead

“…stutzeri LBR and C . metallidurans LBJ are characterized by a high efficiency of lead biosorption compared to the work of Waite and colleagues [ 29 ], who proved that the strain P . stutzeri W228 presents significant results for the absorption of Pb going to concentrations up to 54.0 mg/kg.…”

“…The P. stutzeri LBR strain is used in bioremediation technology in environments affected by heavy metals. It is considered cosmopolitan and it presents a range of biochemical and physiological traits such as nitrogen fixation, denitrification and metal biosorption [19,20]. Moreover, P. stutzeri LBR contributes to the degradation of pollutants and interacts with toxic metals [21].…”

Bacterial communities of Iradiator Gamma Karet Alam (IRKA) pool water

“…Sommer et al [28] recorded that Pseudomonas stutzeri has a similar physiology in terms of growth rate and nutritional requirements to E. coli and thus has a comparable success rate in being a production host for membrane proteins. In addition, these bacteria also known to be able to bioabsorb Pb [29], improve shrimp farming water quality by converting ammonia and nitrite to nitrate [30], produce protease [31] and amylase [32], bio-adsorption of direct red 80 dye from the Jumputan fabric industry [33], and produce auxin phytohormones [34].…”