Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication

Wang, Biao; Wang, Renrui; Cui, Zhenhua; Bi, Wen‐Lu; Li, Jingwei; Li, Bai-Quan; Özüdoğru, Elif Aylin; Volk, Gayle M.

doi:10.1016/j.biotechadv.2014.03.003

Cited by 85 publications

(60 citation statements)

References 118 publications

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“…Organized tissues such as shoot tips are generally considered to have higher genetic stability than embryogenic tissues (Panis and Lambardi 2006;Wang et al 2014a). Somatic embryos derived from shoot tips may therefore be more genetically stable (Rani and Raina 2000;Sticklen and Oraby 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Somatic embryogenesis has been used for micropropagation (Rout et al 2006), genetic transformation (Panis et al 1993;Nhut et al 2001;Wang et al 2012Wang et al , 2014a, and artificial seed production (Kumar and Thomas 2012;Cheruvathur et al 2013). For most applications, in addition to efficient embryogenic callus induction, regenerative ability must be maintained through repeated subcultures.…”

Section: Introductionmentioning

confidence: 99%

“…Cryopreservation of embryogenic tissues may provide a solution to these problems (Lambardi et al 2008;Panis 2009;Feng et al 2011;Wang et al 2012Wang et al , 2014a. Over the last three decades, great efforts have been made to cryopreserve embryogenic tissues and various cryopreservation protocols have been developed in many economically important crops (Lambardi et al 2008;Feng et al 2011;Wang et al 2012Wang et al , 2014a.…”

Section: Introductionmentioning

confidence: 99%

“…Over the last three decades, great efforts have been made to cryopreserve embryogenic tissues and various cryopreservation protocols have been developed in many economically important crops (Lambardi et al 2008;Feng et al 2011;Wang et al 2012Wang et al , 2014a. However, some plant species such as Lilium are still recalcitrant to cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

“…Shoot tips are preferred over cells and calluses for cryopreservation of genetic resources, because of their high genetic stability (Sticklen and Oraby 2005;Panis and Lambardi 2006;Wang et al 2014a). In previous studies on shoot tip cryopreservation, plant regeneration was achieved only from shoot regrowth (Benson 2008;Reed 2008;Wang et al 2014a). Therefore, an interesting question arises as to whether de novo somatic embryos can be produced from shoot tips following cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

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Plant regeneration from shoot regrowth and de novo embryo-like structures from cryopreserved shoot tips of Lilium spp.

Yin

Guo

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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Plant regeneration via both shoot regrowth and de novo embryo-like structures was obtained from cryopreserved shoot tips of Lilium spp. Shoot tips (1.0-3.0 mm in length) of Lilium Oriental hybrid 'Siberia' containing two-to four-leaf primordia were excised from 4-wk-old adventitious shoots and cryopreserved by droplet vitrification. When postcultured for 6 wk on recovery medium containing 0.1 mg/l α-naphthaleneacetic acid (NAA) in combination with different kinetin (KT) concentrations (0-0.4 mg/l), three types of regenerants were produced: (1) only embryo-like structures, (2) shoot regrowth with embryo-like structures, and (3) only shoot regrowth. The highest frequencies (≥75.0%) of total embryo-like structures were obtained from cryopreserved shoot tips post-cultured on recovery medium containing 0.1 mg/l NAA and 0.1-0.2 mg/l KT. Embryo-like structures proliferated within 4 wk of culture on medium containing 1.0 mg/l NAA and 0.05 mg/l thidiazuron (TDZ). Somatic embryo-like structures matured and converted into whole plantlets after 8 wk of culture on Murashige and Skoog (MS) medium without any plant growth regulators. The highest frequencies (≥25.0%) of total shoot regrowth were produced from cryopreserved shoot tips post-cultured on recovery medium containing 0.1 mg/l NAA and 0.05-0.4 mg/l KT. With the protocols reported here, frequencies of total embryo-like structures and shoot regrowth ranged from 45.0 to 90.0% and 25.0 to 52.5%, respectively, from six lily varieties belonging to five Lilium species or hybrids, representing diverse Lilium germplasm. The results reported here have potential applications to long-term conservation of genetic resources and genetic transformation in Lilium spp.

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Section: Discussionmentioning

confidence: 99%