Potential biomarkers for the early diagnosis of colorectal adenocarcinoma – transcriptomic analysis of four clinical stages

Janikowska, Grażyna; Janikowski, Tomasz; Pyka, Alina; Mazurek, Urszula; Janikowski, Marcin; Gonciarz, Maciej; Lorenc, Z. Paul

doi:10.3233/cbm-170984

Cited by 14 publications

(10 citation statements)

References 40 publications

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“…Lck expression was detected in a number of solid cancers including breast cancer [113,114,115,116], colon cancer [117,118,119], and lung carcinoma [119,120,121]. These observations have led to the hypothesis that Lck may have cancer promoting functions and hence may represent a potential diagnostic biomarker and therapeutic target for solid cancers.…”

Section: The Role Of Lck In Tumors Of Non-hematopoietic Originmentioning

confidence: 99%

Beyond TCR Signaling: Emerging Functions of Lck in Cancer and Immunotherapy

Bommhardt

Schraven

Simeoni

2019

IJMS

115

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In recent years, the lymphocyte-specific protein tyrosine kinase (Lck) has emerged as one of the key molecules regulating T-cell functions. Studies using Lck knock-out mice or Lck-deficient T-cell lines have shown that Lck regulates the initiation of TCR signaling, T-cell development, and T-cell homeostasis. Because of the crucial role of Lck in T-cell responses, strategies have been employed to redirect Lck activity to improve the efficacy of chimeric antigen receptors (CARs) and to potentiate T-cell responses in cancer immunotherapy. In addition to the well-studied role of Lck in T cells, evidence has been accumulated suggesting that Lck is also expressed in the brain and in tumor cells, where it actively takes part in signaling processes regulating cellular functions like proliferation, survival and memory. Therefore, Lck has emerged as a novel druggable target molecule for the treatment of cancer and neuronal diseases. In this review, we will focus on these new functions of Lck.

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Section: The Role Of Lck In Tumors Of Non-hematopoietic Originmentioning

confidence: 99%

Beyond TCR Signaling: Emerging Functions of Lck in Cancer and Immunotherapy

Bommhardt

Schraven

Simeoni

2019

IJMS

115

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“…LAMP2 mediates the fusion of autophagosomes and lysosomes during autophagy and plays an important role in autophagosome maturation ( 34 ). In colon cancer, LAMP2 is differentially expressed and has specific diagnostic value in the early stage of colon cancer as a molecular marker ( 35 ). In breast cancer, high expression of LAMP2 is also detected, and its high expression is significantly associated with tumor progression ( 36 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-1-3p Suppresses Malignant Phenotypes of Ameloblastoma Through Down-Regulating Lysosomal Associated Membrane Protein 2-Mediated Autophagy

et al. 2021

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Objective: Several clinical trials have suggested that autophagy inhibition is a promising approach for cancer therapy. However, the implications of autophagy in ameloblastoma (AB) remain undiscovered. This study investigated the dysregulated autophagy and its regulatory mechanisms in AB.Methods: The expression and distribution of autophagy-related proteins including B-cell lymphoma-2-interacting protein-1 (Beclin1), microtubule-associated protein 1 light chain 3 (LC3) II/I and lysosomal associated membrane protein 2 (LAMP2) were detected in AB and normal oral mucosa (NOM) tissues by immunohistochemistry and western blot analyses. Under transmission electron microscopy, the autophagy of AB was observed. LAMP2 was a potential target mRNA of miR-1-3p. Quantitative Real-time PCR (qRT-PCR) analysis was utilized for examining LAMP2 and miR-1-3p in AB tissues as well as AM-1 cells. The correlation between LAMP2 and miR-1-3p was analyzed in AB. After transfection with miR-1-3p mimic or inhibitor, LAMP2 expression, proliferation, migration, and invasion were separately detected in AM-1 cells. Rescue assays were finally presented.Results: Our results showed that Beclin1 was lowly expressed as well as LC3II/I and LAMP2 were highly expressed in AB. Autophagosomes were observed in AB. MiR-1-3p was lowly expressed in AB, which exhibited negative correlations to LAMP2 expression. MiR-1-3p up-regulation significantly lowered LAMP2 expression in AM-1 cells. Furthermore, miR-1-3p overexpression restrained proliferative, migrated, and invasive capacities of AM-1 cells, which were ameliorated by LAMP2 overexpression.Conclusion: Our findings demonstrated that miR-1-3p suppressed malignant phenotypes of AB through down-regulating LAMP2-mediated autophagy, which could become an underlying target for AB therapy.

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“…It is correlated to the ability of NK-extracellular vesicles (EV) to induce cytotoxicity [35]. Additionally, serum GNLY may be a potential biomarker for nasopharyngeal carcinoma, the early stage of colorectal adenocarcinoma and muscle-invasive bladder cancer [36][37][38]. The function of GNLY in TNBC immune microenvironment and immunotherapies deserves further research.…”

Section: Discussionmentioning

confidence: 99%

Immune Microenvironment-Related Genes Contribute to Clinical Prognosis in Patients with Triple-Negative Breast Cancer

Feng¹,

Gao²,

Yang³

et al. 2021

Preprint

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Background:Triple-negative breast cancer (TNBC) is a high-risk breast cancer subtype, which accounts for 15% to 20% of all breast cancers and generally has a poor prognosis. TNBC patients have high recurrence post-surgery and high risk of metastasis to other organs. The tumor microenvironment (TME) plays important roles in the carcinogenesis, development, and metastasis of tumors. Methods: This study aimed to investigate the effects of immune and stromal cell-related genes on TNBC prognosis. The ESTIMATE algorithm was used to calculate the immune/stromal scores of TNBC samples from the GEO database. The samples were divided into high- and low- score groups and the differential expression genes were identified using the limma package within R. Functional enrichment and protein-protein interaction (PPI) network analyses revealed that these genes are primarily involved in immune responses. Results:Survival analysis in both GSE21653 and KM-plotter website showed that 36 genes were significantly related to disease-free survival (DFS) of TNBC. Another survival analysis by R package survival in GSE58812 indicated that 14 genes of 36 were greatly interrelated to DFS of TNBC. Moreover, the expression levels of some of these genes were verified through immunohistochemical staining and RT-qPCR. Finally, four genes importantly associated with TNBC prognosis were identified with Cox-LASSO analysis. Time-dependent receiver-operating characteristic (ROC) analysis displayed an area under the curve (AUC) of 0.95 for one-year survival rate, indicating the four genes performed very well for prognosis prediction. Conclusions:In conclusion, we identified four genes, including BIRC3, CD8A, GNLY and TRIM22, that are possibly associated with the TME and are potential prognostic and therapeutic markers of TNBC.

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Potential biomarkers for the early diagnosis of colorectal adenocarcinoma – transcriptomic analysis of four clinical stages

Abstract: The expression of LCK, GNLY, SLC6A6 and LAMP2 genes could be valuable potential diagnostic biomarkers of the early stage of colorectal adenocarcinoma.

Cited by 14 publications

References 40 publications

Beyond TCR Signaling: Emerging Functions of Lck in Cancer and Immunotherapy

Beyond TCR Signaling: Emerging Functions of Lck in Cancer and Immunotherapy

MicroRNA-1-3p Suppresses Malignant Phenotypes of Ameloblastoma Through Down-Regulating Lysosomal Associated Membrane Protein 2-Mediated Autophagy

Immune Microenvironment-Related Genes Contribute to Clinical Prognosis in Patients with Triple-Negative Breast Cancer

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