Potential Bone Regenerative Effects of DFDBA, Simvastatin and Platelet Rich Fibrin, Radiographically and Histologically of Intra-Bony Periodontal Defects in White New Zealand Rabbits

Abstract

AIM: This study aimed to evaluate and to compare the regenerative power of simvastatin, Demineralized Freeze-Dried Bone Allograft (DFDBA) allograft, platelets rich Fibrin (PRF), and a combination of these materials radiographically and histologically in the intra-bony periodontal defects in white New Zealand rabbits. MATERIALS AND METHODS: This study was conducted on 54 defects in 27 adult male rabbits (n = 27) which were divided into three groups according to the follow-up preplanned scheduled for 1, 2, and 3 weeks. The selected materials were induced as following: A=DFDBA, B=Simvastatin, C= PRF, D=A+C, E=B+C, and F=negative (control group). The intra-bony periodontal defects were induced as the form of one osseous wall defect of 10 mm height, 4 mm depth between the first and the second molars. Then, samples were prepared for histological evaluation. Radiographic assessment was done using computed tomography radiography which was carried at different time intervals as the following baseline, 1, 2, and 3 weeks later. Statistical analysis was performed using ANOVA. RESULTS: After evaluating the results, macroanatomy, radiographically, and histologically, it is thus confirmed that DFDBA allograft combined with PRF create the best bone regenerative results, followed by DFDBA, Simvastatin, simvastatin+ PRF, control group, and finally PRF. CONCLUSION: All of the materials examined in this study showed different percentage in terms of bone density and bone regenerative effects. However, the best results for bone density of the DFDBA + PRF group were recorded after 3 weeks. Thus, the study concludes that a combination of DFDBA + PRF reflects the best properties of both materials in terms of bone density results of the defect. Such results are particularly significant for the selection of bone regeneration materials, and generally, for periodontal regeneration.

Biphasic Calcium Phosphate Versus Demineralized Freeze-Dried Bone Allograft in the Treatment of Periodontal Disease: A Clinical and Radiographical Evaluation

“…The intergroup comparison showed a more significant percentage of bone fill in site 2, which was statistically significant. Similar results were observed in a study done by El-Dien et al [ 53 ].…”

“…The results are analogous to those in the studies of Aspriello et al [ 51 ] and Jayakumar et al [ 52 ], which examined enamel matrix derivative (EMD) and demineralized freeze-dried bone allografts (DFDBA) with DFDBA alone for the treatment of defects. The results are also similar to an animal study conducted by El-Dien et al [ 53 ], in which bilateral defects were arbitrarily treated with DFDBA + platelet-rich plasma (PRP) (test) or DFDBA alone (control). The bone fill at site 2 (DFDBA) was 4.6 ± 1.07 mm at six months, which is in line with previous studies [ 45 , 46 , 48 - 50 , 54 ].…”

Comparative clinical evaluation of xenograft (Cerabone) versus allograft combined with platelet-rich fibrin for treatment of grade II mandibular furcation defects

“…DFDBA, due to its osteoconductive and osteoinductive properties, encourages the development of new attachment systems in infrabony defects. The bone morphogenetic proteins (BMPs) and growth factors that are exposed after the allograft's acid demineralization process are thought to be responsible for the osteoinductive property, which allows for rapid revascularization and the ingrowth of hard tissue in osseous deformities, consequently boosting periodontal regeneration [7].…”

“…Combining platelet-rich fibrin (PRF) with bone grafts in a variety of regenerative surgeries is well-documented in the literature [6][7][8][9]. Nevertheless, inadequate evidence is available comparing the clinical efficacy of naturally derived bovine hydroxyapatite with the PRF for the treatment of furcation defects.…”

Regenerative therapy of Rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin

“…The plateletrich fibrin was an autologous platelet concentrate (Second Generation) and considered as a physiological bio-scaffold that comprised of a three-dimensional fibrin network that rich in integrated platelets, leukocyte, cytokines, as well as the clot they generate which were required for regeneration and healing. [9][10][11][12][13].…”