Potential Broad-Spectrum Antimicrobial, Wound Healing, and Disinfectant Cationic Peptide Crafted from Snake Venom

Abstract

Antimicrobial cationic peptides are intriguing and propitious antibiotics for the future, even against multidrug-resistant superbugs. Venoms serve as a source of cutting-edge therapeutics and innovative, unexplored medicines. In this study, a novel cationic peptide library consisting of seven sequences was designed and synthesized from the snake venom cathelicidin, batroxicidin (BatxC), with the inclusion of the FLPII motif at the N-terminus. SP1V3_1 demonstrated exceptional antibacterial effectiveness against Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae and destroyed the bacteria by depolarizing, rupturing, and permeabilizing their membranes, as evident from fluorescence assays, atomic force microscopy, and scanning electron microscopy. SP1V3_1 was observed to modulate the immune response in LPS-elicited U937 cells and exhibited good antibiofilm activity against MRSA and K. pneumoniae. The peptide promoted wound healing and disinfection in the murine model. The study demonstrated that SP1V3_1 is an exciting peptide lead and may be explored further for the development of better therapeutic peptides.

Multi-Faceted Antimicrobial Efficacy of a Quinoline-Derived Bidentate Copper(II) Ligand Complex and Its Hydrogel Encapsulated Formulation in Methicillin-Resistant Staphylococcus aureus Inhibition and Wound Management

“…The cellular viability assay provided evidence of biocompatibility of SG-Cu as compared to the clinically used drug vancomycin (Figure B). At the MIC of 25 μM, all three cell lines HEK 293, WI38, and NIH/3T3 showed high percentages of cellular viabilities of ∼90%, ∼81%, and ∼86%, respectively.…”

“…Multidrug-resistant superbugs are bacteria that are resistant to three or more classes of antibiotics. According to the O’Neill Report, a review published in 2014, and commissioned by the United Kingdom government, it was predicted that by 2050, infections from bacteria will cause around 10 million more deaths than cancer and diabetes combined . Methicillin-resistant Staphylococcus aureus (MRSA) is a Gram-positive superbug from the ESKAPE group of pathogens responsible for the deadliest hospital-acquired infections (HAIs).…”

Synergistic Augmentation of Beta-Lactams: Exploring Quinoline-Derived Amphipathic Small Molecules as Antimicrobial Potentiators against Methicillin-Resistant Staphylococcus aureus

“…To study the membrane depolarization, the following protocol was followed. 50 MRSA was grown up to an OD 600 of 0.3 at 37 °C. After centrifugation, the bacterial cells were twice washed in washing buffer (20 mM glucose, 5 mM HEPES, pH 7.2) and then resuspended in the same solution to achieve an OD 600 of 0.05 with the addition of 0.1 mol L −1 KCl.…”

“…The membrane potential-sensitive dye DiSC 3 (5) was used in independent cytoplasmic membrane depolarization assays on SG-B-22, SG-B-52, and their combination sets with AMP/AMOX (TCI India). To study the membrane depolarization, the following protocol was followed . MRSA was grown up to an OD 600 of 0.3 at 37 °C.…”

Cationic and amphiphilic peptide-based hydrogels with dual activities as anticancer and antibacterial agents

“…19 These peptide gelators form hydrogels with micro- and nanofibril network structures under appropriate conditions through the self-assembly of individual molecules by using various noncovalent interactions including hydrogen bonding, π–π interactions, van der Waals interactions, hydrophobic interactions and others. 20–24 These hydrogels have a wide range of applications in the biomedical field, such as sustained release of drugs and important biomolecules, 25–28 drug delivery, 25,29 cell culture, 27 tissue engineering, 1,29 wound healing, 19,32 and antimicrobial, 30–40 antiparasitic 37,42 and anticancer agents. 41–48 The self-assembled nanostructures show a remarkably increased bioactivity compared to the unassociated peptide molecules.…”