Potential contribution of strigolactones in regulating scion growth and branching in grafted grapevine in response to nitrogen availability

Cochetel, Noé; Météier, Eloïse; Merlin, Isabelle; Hévin, Cyril; Pouvreau, Jean‐Bernard; Coutos-Thévenot, Pierre; Hernould, Michel; Vivin, Philippe; Cookson, Sarah Jane; Ollat, Nathalie; Lauvergeat, Virginie

doi:10.1093/jxb/ery206

Cited by 25 publications

(17 citation statements)

References 58 publications

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“…In woody plant, poplar MAX4 (CCD8) knockdown lines exhibited altered branching patterns [24]. In grape, it has been suggested that SL could be involved in the control of scion architecture in grafted grapevine plants, based on the observation that exudate from grape CCDs-overexpressing transgenic cells could stimulate the germination of Phelipanche ramosa seeds, and that overexpression of grape CCD7 or CCD8 gene in corresponding Arabidopsis mutant can partly revert the mutant phenotype [25]. However, no direct and clear evidence supporting this role exists in grapevine.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, SLs were proposed to control scion development in response to nitrogen availability in grafted grapevine plants [25]. Additionally, overexpression of grape CCD7 or CCD8 gene in Arabidopsis max3 or max4 mutants background partly reverted their phenotypes [25], suggesting a potential role for CCD7 and CCD8 in grapevine shoot branching. However, up to date, in grapevine no experimental evidence supporting the role of these two genes in the control of shoot branching exists.…”

Section: Introductionmentioning

confidence: 99%

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Knockout of VvCCD8 gene in grapevine affects shoot branching

et al. 2020

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Background: Shoot branching is an important trait of plants that allows them to adapt to environment changes. Strigolactones (SLs) are newly identified plant hormones that inhibit shoot branching in plants. The SL biosynthesis genes CCD7 (carotenoid cleavage dioxygenase 7) and CCD8 have been found to regulate branching in several herbaceous plants by taking advantage of their loss-of-function mutants. However, the role for CCD7 and CCD8 in shoot branching control in grapevine is still unknown due to the lack of corresponding mutants. Results: Here we employed the CRISPR/Cas9 system to edit the VvCCD7 and VvCCD8 genes in the grape hybrid 41B. The 41B embryogenic cells can easily be transformed and used for regeneration of the corresponding transformed plants. Sequencing analysis revealed that gene editing has been used successfully to target both VvCCD genes in 41B embryogenic cells. After regeneration, six 41B plantlets were identified as transgenic plants carrying the CCD8-sgRNA expression cassette. Among these, four plants showed mutation in the target region and were selected as ccd8 mutants. These ccd8 mutants showed increased shoot branching compared to the corresponding wild-type plants. In addition, no off-target mutation was detected in the tested mutants at predicted off-target sites. Conclusions: Our results underline the key role of VvCCD8 in the control of grapevine shoot branching.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Knockout of VvCCD8 gene in grapevine affects shoot branching

et al. 2020

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“…A previous study on Pi (an inorganic form of phosphorus) reported that strigolactones can regulate root systems by improving development of lateral root formation with a suitable concentration of Pi and conversely inhibit the emergence of lateral roots with a high concentration of Pi. Thus, reducing the uptake of heavy metals in the plant by decreasing the length and density of root hairs is one of the avoidance mechanism of strigolactones for coping with heavy metals [95][96][97][98][99][100][101]. Recent research has confirmed the role of strigolactones (SLs) on the LR (latest root) formation process, which occurs in synthetic strigolactone stages [27].…”

Section: Strigolactones (Sls) Via Heavy Metal Stressmentioning

confidence: 99%

“…Exogenous GR24 leads to increasing plant tolerance against salt and drought stress in Arabidopsis . When placed in the root access, it is transferred to the shoots , creating a positive role in coping with stress [101]. A previous experiment found that GR24 reduced MDA content and chloroplast damage and increased the photosynthetic capacity and plant defense mechanism against ROS with the stimulation of antioxidant enzyme activity (SOD and POD) caused by salinity stresses [1].…”

Section: Strigolactones (Sls) Via Heavy Metal Stressmentioning

confidence: 99%

The Role of New Members of Phytohormones in Plant Amelioration under Abiotic Stress with an Emphasis on Heavy Metals

Emamverdian¹,

Ding²,

Xie³

2020

Pol. J. Environ. Stud.

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In addition to regulating plant growth and development, phytohormones play an essential role in the response to abiotic and biotic stress-especially heavy metal stress. In response to environmental stressors, phytohormones act as signaling molecules in both exogenous and endogenous signaling pathways. In parts of the rhizosphere, phytohormones have an exogenous role, and when regulating plant growth and development, they have an endogenous role. Phytohormones and their associated signaling pathway network are involved in plant responses to heavy metal stress. These molecules can improve the plant defense system by increasing antioxidant enzyme activity and degrading lipoperoxidation and H 2 O 2. Phytohormones are divided into classical phytohormones such as auxins, ethylene, gibberellins, and cytokinins, that have been investigated for many years and the new members, such as jasmonates, brassinosteroids, and strigolactones, which have been little studied. In this review article, we investigated the main mechanisms involved in the amelioration of heavy metals by the three new phytohormones to provide more knowledge about their detoxification mechanisms.

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“…Several SL-driven developmental modules have been shown to be highly conserved in the plant kingdom, like shoot lateral branching, root hair elongation, leaf aging and crosstalk with most phytohormones. The recent development of SL-based strategies seems endless, going from approaches targeted to the improvement of good agricultural practices (Pavan et al, 2016; Cochetel et al, 2018; Liu et al, 2018a; Mohemed et al, 2018) to the amelioration of human health through the recently revealed apoptotic function of SL mimics in different kinds of cancers and diseases (Modi et al, 2017, 2018; Hasan et al, 2018).…”

Section: Abcg Subfamily and Strigolactone Transportmentioning

confidence: 99%

Filling the Gap: Functional Clustering of ABC Proteins for the Investigation of Hormonal Transport in planta

2019

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Plant hormones regulate a myriad of plant processes, from seed germination to reproduction, from complex organ development to microelement uptake. Much has been discovered on the factors regulating the activity of phytohormones, yet there are gaps in knowledge about their metabolism, signaling as well as transport. In this review we analyze the potential of the characterized phytohormonal transporters belonging to the ATP-Binding Cassette family (ABC proteins), thus to identify new candidate orthologs in model plants and species important for human health and food production. Previous attempts with phylogenetic analyses on transporters belonging to the ABC family suggested that sequence homology per se is not a powerful tool for functional characterization. However, we show here that sequence homology might indeed support functional conservation of characterized members of different classes of ABC proteins in several plant species, e.g., in the case of ABC class G transporters of strigolactones and ABC class B transporters of auxinic compounds. Also for the low-affinity, vacuolar abscisic acid (ABA) transporters belonging to the ABCC class we show that localization-, rather than functional-clustering occurs, possibly because of sequence conservation for targeting the tonoplast. The ABC proteins involved in pathogen defense are phylogenetically neighboring despite the different substrate identities, suggesting that sequence conservation might play a role in their activation/induction after pathogen attack. Last but not least, in case of the multiple lipid transporters belong to different ABC classes, we focused on ABC class D proteins, reported to transport/affect the synthesis of hormonal precursors. Based on these results, we propose that phylogenetic approaches followed by transport bioassays and in vivo investigations might accelerate the discovery of new hormonal transport routes and allow the designing of transgenic and genome editing approaches, aimed to improve our knowledge on plant development, plant–microbe symbioses, plant nutrient uptake and plant stress resistance.

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Potential contribution of strigolactones in regulating scion growth and branching in grafted grapevine in response to nitrogen availability

Abstract: Grapevine rootstocks regulate scion growth and architecture differently in response to nitrogen availability. Strigolactones are suggested as key root-derived molecules modulating scion development in grafted grapevine.

Cited by 25 publications

References 58 publications

Knockout of VvCCD8 gene in grapevine affects shoot branching

Knockout of VvCCD8 gene in grapevine affects shoot branching

The Role of New Members of Phytohormones in Plant Amelioration under Abiotic Stress with an Emphasis on Heavy Metals

Filling the Gap: Functional Clustering of ABC Proteins for the Investigation of Hormonal Transport in planta

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