Potential-dependent extracellular electron transfer pathways of exoelectrogens

Liu, Dongfeng; Li, Wen-Wei

doi:10.1016/j.cbpa.2020.06.005

Cited by 38 publications

(10 citation statements)

References 48 publications

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“…When studying these bacteria in a bioelectrochemical system (BES), they were found to also respire through electrodes. The chosen conditions of the BES, like available substrates and electric potential of the electrode, can be tuned to enhance the nanowire production ( Liu and Li, 2020 ; Yalcin et al, 2020 ). These bacterial nanowires are found in many forms and debate is still going on about its exact function and chemical structure ( Creasey et al, 2018 ).…”

Section: Electroactive Bacteria For Bioelectronicsmentioning

confidence: 99%

Biomaterials and Electroactive Bacteria for Biodegradable Electronics

et al. 2022

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The global production of unrecycled electronic waste is extensively growing each year, urging the search for alternatives in biodegradable electronic materials. Electroactive bacteria and their nanowires have emerged as a new route toward electronic biological materials (e-biologics). Recent studies on electron transport in cable bacteria—filamentous, multicellular electroactive bacteria—showed centimeter long electron transport in an organized conductive fiber structure with high conductivities and remarkable intrinsic electrical properties. In this work we give a brief overview of the recent advances in biodegradable electronics with a focus on the use of biomaterials and electroactive bacteria, and with special attention for cable bacteria. We investigate the potential of cable bacteria in this field, as we compare the intrinsic electrical properties of cable bacteria to organic and inorganic electronic materials. Based on their intrinsic electrical properties, we show cable bacteria filaments to have great potential as for instance interconnects and transistor channels in a new generation of bioelectronics. Together with other biomaterials and electroactive bacteria they open electrifying routes toward a new generation of biodegradable electronics.

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Section: Electroactive Bacteria For Bioelectronicsmentioning

confidence: 99%

Biomaterials and Electroactive Bacteria for Biodegradable Electronics

et al. 2022

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“…Geobacter species, as the predominant microorganisms for Fe(III) and Mn(IV) reduction in the natural environment, fill key niches in the soils, sediments, and groundwater. − In addition, they possess powerful abilities in radionuclide immobilization, toxic metal detoxification, and electrical interaction with solid electrodes or minerals. − The versatile sensory networks, respiratory chains, and metabolic reactions further strengthen their ability to adapt to a diverse and variable environment. − All of these favorable features make them important players in elemental biogeochemical cycling and environmental bioremediation. However, the highly complicated, dynamic pathways of sensing, metabolism, and respiration also severely hinder a deep understanding and effective engineering of these bacteria.…”

Section: Introductionmentioning

confidence: 99%

Biomolecular Insights into Extracellular Pollutant Reduction Pathways of Geobacter sulfurreducens Using a Base Editor System

Cheng

et al. 2022

Environ. Sci. Technol.

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Geobacter species are critically involved in elemental biogeochemical cycling and environmental bioremediation processes via extracellular electron transfer (EET), but the underlying biomolecular mechanisms remain elusive due to lack of effective analytical tools to explore into complicated EET networks. Here, a simple and highly efficient cytosine base editor was developed for engineering of the slow-growing Geobacter sulfurreducens (a doubling time of 5 h with acetate as the electron donor and fumarate as the electron acceptor). A single-plasmid cytosine base editor (pYYDT-BE) was constructed in G. sulfurreducens by fusing cytosine deaminase, Cas9 nickase, and a uracil glycosylase inhibitor. This system enabled single-locus editing at 100% efficiency and showed obvious preference at the cytosines in a TC, AC, or CC context than in a GC context. Gene inactivation tests confirmed that it could effectively edit 87.7–93.4% genes of the entire genome in nine model Geobacter species. With the aid of this base editor to construct a series of G. sulfurreducens mutants, we unveiled important roles of both pili and outer membrane c-type cytochromes in long-range EET, thereby providing important evidence to clarify the long-term controversy surrounding their specific roles. Furthermore, we find that pili were also involved in the extracellular reduction of uranium and clarified the key roles of the ExtHIJKL conduit complex and outer membrane c-type cytochromes in the selenite reduction process. This work developed an effective base editor tool for the genetic modification of Geobacter species and provided new insights into the EET network, which lay a basis for a better understanding and engineering of these microbes to favor environmental applications.

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“…This network allows the formation of conductive biofilms in which also the outer cell layers are electrically “wired” to the anode [13] [14] . It was shown that the applied potential influences the type of Omc active in electron transport as well as the composition of the extracellular polymeric substances present in the biofilm [15] [16] [17] . What has been little discussed so far, is the interpolated effect of electrode material and applied potential.…”

Section: Introductionmentioning

confidence: 99%

R-based method for quantitative analysis of biofilm thickness by using Confocal Laser Scanning Microscopy

Frühauf

Stöckl

Holtmann

2022

Preprint

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Microscopy is mostly the method of choice to analyse biofilms. Due to the high local heterogeneity of biofilms, single and punctual analyses only give an incomplete insight into the local distribution of biofilms. In order to retrieve statistically significant results a quantitative method for biofilm thickness measurements was developed based on confocal laser scanning microscopy and the programming language R. The R-script allows the analysis of large image volumes with little hands-on work and outputs statistical information on homogeneity of surface coverage and overall biofilm thickness. The applicability of the script was shown in microbial fuel cell experiments. It was found that G. sulfurreducens responds differently to poised anodes of different material so that the optimum potential for MFC on poised ITO anodes had to be identified with respect to maximum current density, biofilm thickness and MFC start-up time. Thereby, a positive correlation between current density and biofilm thickness was found, but with no direct link to the applied potential. The optimum potential turned out to be +0.1 V vs SHE. The script proved to be a valuable stand-alone tool to quantify biofilm thickness in a statistically valid manner, which is required in many studies.

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Potential-dependent extracellular electron transfer pathways of exoelectrogens

Cited by 38 publications

References 48 publications

Biomaterials and Electroactive Bacteria for Biodegradable Electronics

Biomaterials and Electroactive Bacteria for Biodegradable Electronics

Biomolecular Insights into Extracellular Pollutant Reduction Pathways of Geobacter sulfurreducens Using a Base Editor System

R-based method for quantitative analysis of biofilm thickness by using Confocal Laser Scanning Microscopy

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