Potential for Drug Repositioning of Midazolam for Dentin Regeneration

Karakida, Takeo; Onuma, Kazuo; Saito, Mari; Yamamoto, Ryûji; Chiba, Toshie; Chiba, Risako; Hidaka, Yukihiko; Fujii-Abe, Keiko; Kawahara, Hiroshi; Yamakoshi, Yasuo

doi:10.3390/ijms20030670

Cited by 19 publications

(23 citation statements)

References 43 publications

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“…In order to investigate the dynamics of dental pulp cells on an MTA disk, we used a porcine dental pulp-derived cell line (PPU7) for the present study ( Appendix A.2 ) [ 26 ]. As a first step, we attempted to visualize PPU7 cells on MTA by introducing the DsRed–Express–DR vector into PPU7 cells.…”

Section: Resultsmentioning

confidence: 99%

“…The PPU7 cell line was established from porcine dental pulp cells by our group [ 26 ]. The pDsRed–Express–DR vector (Takara Bio USA Inc., Mountain View, CA, USA) containing a cytomegalovirus promoter in a multicloning site was used for gene transfection.…”

Section: Methodsmentioning

confidence: 99%

“…Since pigs are relatively close to humans physiologically and anatomically, they are expected to be used as a model for future regenerative medicine. We previously established the PPU7 cell line from porcine dental pulp cells [ 26 ] and used it for dentin-regeneration research [ 27 , 28 ]. Hence, we used PPU7 cells for basic research to investigate the cell dynamics on the MTA disk.…”

Section: Appendix A1 Fluorescence Microscope Observation Of Dered-pmentioning

confidence: 99%

“…When we previously established the porcine dental pulp cell line, we selected two clones—one with high and one with low inherent ALP activity. The cell line with high inherent ALP activity (i.e., the PPU7 cell line), cultured in the presence of BMP-2 or TGF-β, further upregulated the gene-expression level of ALP but the other cell line, with low ALP activity, had no effect [ 26 ]. This finding indicates that the differentiation of PPU7 cells requires a specific high level of inherent ALP activity.…”

Section: Appendix A1 Fluorescence Microscope Observation Of Dered-pmentioning

confidence: 99%

“…The immortalized porcine dental pulp cell line (PPU7) was established [ 26 ] and has been used for endodontic research associated with laser irradiation [ 27 ]. PPU7 cells can express alkaline phosphatase (ALP) and odontoblastic marker genes that form precipitated nodules when cultured in a medium containing β-glycerophosphate and ascorbic acid.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate

Hattori-Sanuki

Karakida

Chiba-Ohkuma

et al. 2020

Cells

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Mineral trioxide aggregate (MTA) was introduced as a material for dental endodontic regenerative therapy. Here, we show the dynamics of living dental pulp cells in direct contact with an MTA disk. A red fluorescence protein (DsRed) was introduced into immortalized porcine dental pulp cells (PPU7) and cloned. DsRed-PPU7 cells were cultured on the MTA disk and cell proliferation, chemotaxis, the effects of growth factors and the gene expression of cells were investigated at the biological, histomorphological and genetic cell levels. Mineralized precipitates formed in the DsRed-PPU7 cells were characterized with crystal structural analysis. DsRed-PPU7 cells proliferated in the central part of the MTA disk until Day 6 and displayed a tendency to move to the outer circumference. Both transforming growth factor beta and bone morphogenetic protein promoted the proliferation and movement of DsRed-PPU7 cells and also enhanced the expression levels of odontoblastic gene differentiation markers. Mineralized precipitates formed in DsRed-PPU7 were composed of calcium and phosphate but its crystals were different in each position. Our investigation showed that DsRed-PPU7 cells in direct contact with the MTA disk could differentiate into odontoblasts by controlling cell–cell and cell–substrate interactions depending on cell adhesion and the surrounding environment of the MTA.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Appendix A1 Fluorescence Microscope Observation Of Dered-pmentioning

confidence: 99%

Section: Appendix A1 Fluorescence Microscope Observation Of Dered-pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate

Hattori-Sanuki

Karakida

Chiba-Ohkuma

et al. 2020

Cells

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Dentin regeneration based on tooth tissue engineering: A review

Azaryan

Razavi

Hanafi‐Bojd

et al. 2023

Biotechnology Progress

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Missing or damaged teeth due to caries, genetic disorders, oral cancer, or infection may contribute to physical and mental impairment that reduces the quality of life.Despite major progress in dental tissue repair and those replacing missing teeth with prostheses, clinical treatments are not yet entirely satisfactory, as they do not regenerate tissues with natural teeth features. Therefore, much of the focus has centered on tissue engineering (TE) based on dental stem/progenitor cells to create bioengineered dental tissues. Many in vitro and in vivo studies have shown the use of cells in regenerating sections of a tooth or a whole tooth. Tooth tissue engineering (TTE), as a promising method for dental tissue regeneration, can form durable biological substitutes for soft and mineralized dental tissues. The cell-based TE approach, which directly seeds cells and bioactive components onto the biodegradable scaffolds, is currently the most potential method. Three essential components of this strategy are cells, scaffolds, and growth factors (GFs). This study investigates dentin regeneration after an injury such as caries using TE and stem/progenitor cell-based strategies. We begin by discussing about the biological structure of a dentin and dentinogenesis.The engineering of teeth requires knowledge of the processes that underlie the growth of an organ or tissue. Then, the three fundamental requirements for dentin regeneration, namely cell sources, GFs, and scaffolds are covered in the current study, which may ultimately lead to new insights in this field.

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