Potential for entomopathogenic fungi to control                         Triatoma dimidiata (Hemiptera: Reduviidae), a                     vector of Chagas disease in Mexico

Vázquez-Martínez, María Guadalupe; Cirerol-Cruz, Blanca Elva; Torres-Estrada, José Luis; López, Mario Henry Rodríguez

doi:10.1590/0037-8682-0193-2014

Cited by 12 publications

(9 citation statements)

References 34 publications

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“…Our results also outperformed those from Lecuona et al (2001) who needed more days to obtain similar results and a higher fungal concentration (>10 8 conidia/ml). Vazquez-Martinez et al (2014) also reported a 90% mortality rate at a RH=75%, but they occurred 19 dpi, while the Pr-11 strain caused a 97% mortality 10 dpi (Figure 2). High mortality rates were obtained by Pedrini et al (2009), but using a 10 9 conidia/ml concentration of the GHA strain (Laverlam International, Butte, USA).…”

Section: Discussionmentioning

confidence: 92%

Isolation of Beauveria bassiana Pr-11 from Andean orthopterans and its effectiveness against Chagas disease vectors in Peru

et al. 2020

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Section: Discussionmentioning

confidence: 92%

Isolation of Beauveria bassiana Pr-11 from Andean orthopterans and its effectiveness against Chagas disease vectors in Peru

et al. 2020

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“…Partially purified chitinases of S. marcescens PRNK-1, an isolated from cockroach, strongly inhibited the fungal growth of Rhizoctonia solani and Fusarium oxysporum in vitro (Moon et al, 2017). Chitinases secreted in the TDT could be especially important to protect its microbiota from infection by entomopathogenic fungi, i.e., Beauveria bassiana, Gliocladium virens, Metarhizium anisopliae , and Isaria fumosorosea (Vázquez-Martínez et al, 2014; Forlani et al, 2015; Huang et al, 2016). Although S. marcescens possess chitinolytic activity over the fungal wall, it is unknown if it affects the perimicrovillar membrane of TDT which also contains chitin (Alvarenga et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…A sustainable alternative strategy to insecticide is biological control. Fungi (Vázquez-Martínez et al, 2014; Forlani et al, 2015; Garcia et al, 2016), and toxin-producing bacteria such as Bacillus thuringiensis are already largely used as commercial biopesticides. The biological control by symbiotic bacteria, such as Wolbachia sp., has also been demonstrated to be effective in the control of Aedes aegypti (McMeniman et al, 2009; Bian et al, 2010; Hancock et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract

et al. 2019

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Serratia marcescens is a bacterium with the ability to colonize several niches, including some eukaryotic hosts. S. marcescens have been recently found in the gut of hematophagous insects that act as parasite vectors, such as Anopheles, Rhodnius, and Triatoma. While some S. marcescens strains have been reported as symbiotic or pathogenic to other insects, the role of S. marcescens populations from the gut microbiota of Rhodnius prolixus, a vector of Chagas’ disease, remains unknown. Bacterial colonies from R. prolixus gut were isolated on BHI agar. After BOX-PCR fingerprinting, the genomic sequences of two isolates RPA1 and RPH1 were compared to others S. marcescens from the NCBI database in other to estimate their evolutionary divergence. The in vitro trypanolytic activity of these two bacterial isolates against Trypanosoma cruzi (DM28c clone and Y strain) was assessed by microscopy. In addition, the gene expression of type VI secretion system (T6SS) was detected in vivo by RT-PCR. Comparative genomics of RPA1 and RPH1 revealed, besides plasmid presence and genomic islands, genes related to motility, attachment, and quorum sensing in both genomes while genes for urea hydrolysis and type II secretion system (T2SS) were found only in the RPA1 genome. The in vitro trypanolytic activity of both S. marcescens strains was stronger in their stationary phases of growth than in their exponential ones, with 65–70 and 85–90% of epimastigotes (Dm28c clone and Y strain, respectively) being lysed after incubation with RPA1 or RPH1 in stationary phase. Although T6SS transcripts were detected in guts up to 40 days after feeding (DAF), R. prolixus morbidity or mortality did not appear to be affected. In this report, we made available two trypanolytic S. marcescens strains from R. prolixus gut to the scientific community together with their genomic sequences. Here, we describe their genomic features with the purpose of bringing new insights into the S. marcescens adaptations for colonization of the specific niche of triatomine guts. This study provides the basis for a better understanding of the role of S. marcescens in the microbiota of R. prolixus gut as a potential antagonist of T. cruzi in this complex system.

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“…Nymphs died after a long period following inoculation (21 days), and the fungus exhibited no sporulation; however, when administered to adults, mortality rose to 75%, with a lower interval (16 days). This difference possibly derived from the loss of conidia, which may have occurred during molting after the topical inoculation of nymphs [58].…”

Section: Experimental Evidence Of Anti-insectan Effectsmentioning

confidence: 99%

Talaromyces–Insect Relationships

Nicoletti

Becchimanzi

2021

Microorganisms

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Facing the urgent need to reduce the input of agrochemicals, in recent years, the ecological relationships between plants and their associated microorganisms have been increasingly considered as an essential tool for improving crop production. New findings and data have been accumulated showing that the application of fungi can go beyond the specific role that has been traditionally assigned to the species, employed in integrated pest management as entomopathogens or mycoparasites, and that strains combining both aptitudes can be identified and possibly used as multipurpose biocontrol agents. Mainly considered for their antagonistic relationships with plant pathogenic fungi, species in the genus Talaromyces have been more and more widely reported as insect associates in investigations carried out in various agricultural and non-agricultural contexts. Out of a total of over 170 species currently accepted in this genus, so far, 27 have been found to have an association with insects from 9 orders, with an evident increasing trend. The nature of their mutualistic and antagonistic relationships with insects, and their ability to synthesize bioactive compounds possibly involved in the expression of the latter kind of interactions, are analyzed in this paper with reference to the ecological impact and applicative perspectives in crop protection.

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Potential for entomopathogenic fungi to control Triatoma dimidiata (Hemiptera: Reduviidae), a vector of Chagas disease in Mexico

Cited by 12 publications

References 34 publications

Isolation of Beauveria bassiana Pr-11 from Andean orthopterans and its effectiveness against Chagas disease vectors in Peru

Isolation of Beauveria bassiana Pr-11 from Andean orthopterans and its effectiveness against Chagas disease vectors in Peru

In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract

Talaromyces–Insect Relationships

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