Potential for inoculation of common bean by effective rhizobia in Tunisian soils

Aouani, Mohamed Elarbi; Mhamdi, Ridha; Mars, M.; Elayeb, Mohamed; Ghtir, R.

doi:10.1051/agro:19970902

Cited by 21 publications

(20 citation statements)

References 11 publications

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“…The Sinorhizobium meliloti (SMCP) strain had the lowest effectiveness with all analysed chickpea cultivars. This variability of effectiveness, observed among symbioses, agreed with results of our previous studies (Mhadhbi et al 2004) and other reports on chickpea-rhizobia (Soussi et al 1999) and common bean-rhizobia symbioses (Aouani et al 1997;Tejera et al 2004).…”

Section: Resultssupporting

confidence: 93%

“…The selection of effective rhizobial strains can contribute to the improvement of symbiotic nitrogen fixation under stress (Serraj et al 1999;Zahran 1999). In Tunisia, several works of exploration, physiological and biochemical analyses and field trials have been performed to enhance symbiotic-dependent legume production in important agronomic regions (Aouani et al 1997Jebara et al 2001;Mhamdi et al 2002;Mhadhbi et al 2004;Mrabet et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Symbiotic effectiveness and response to mannitol-mediated osmotic stress of various chickpea–rhizobia associations

Mhadhbi

Jebara

Zitoun

et al. 2007

World J Microbiol Biotechnol

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Thirty-six symbiotic associations involving six chickpea cultivars against six rhizobial strains were evaluated for symbiotic performance and responses to osmotic stress applied by mannitol (50 mM) in aerated hydroponic cultures. Analyses in different symbioses were focused on biomass production, nodulation, nitrogen fixation, and their modulation under osmotic stress conditions, as well as expression of nodular antioxidant enzymes. Mesorhizobium ciceri reference (835) and local (CMG6) strains, as well as the local (C 11 ) M. mediterraneum allowed the best symbiotic efficiency for all chickpea cultivars. The osmotic stress induces severe decrease ranging 30-50% in aerial biomass and 50-70% for nitrogen fixation. Nevertheless, plants inoculated with M. ciceri (835) and M. mediterraneum (C 11 ) preserve a relatively high growth (4 g plant -1 ) with nitrogen-fixing activity (25 lmols h -1 plant -1 ). The bacterial partner was the most important factor of variance of the analysed parameters in osmotic stress or physiological conditions where it gets to 60-85%. The strains allowing the best competent symbioses were proposed for field assays. Under osmotic stress, nodular peroxidase (POX) and ascorbate peroxidase (APX) activities were significantly enhanced. The increase of POX and APX was inversely correlated with the inhibition of aerial biomass production (p = 0.05) and nitrogen-fixing capacity (p = 0.01), suggesting a protective role of these enzymes in nodules. Superoxide dismutase (SOD) was also activated in stressed nodules. However, the spectacular decrease in catalase (CAT) activity discounts its involvement in osmotic stress response.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Symbiotic effectiveness and response to mannitol-mediated osmotic stress of various chickpea–rhizobia associations

Mhadhbi

Jebara

Zitoun

et al. 2007

World J Microbiol Biotechnol

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“…Bacterial partner influence on symbiosis performance was mentioned in several reports; hence, (Aouani et al 1997) grouped rhizobial strains according to their effectiveness on common bean cultivar. Differences in strain effectiveness can be associated with compatibility with host plant controlled by a complex interaction mechanism (Hirsh et al 2001;Denarié et al 1992).…”

Section: Discussionmentioning

confidence: 99%

The potential of Rhizobium strains for biological control of Orobanche crenata

et al. 2007

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Orobanche crenata Forsk is a chlorophyll lacking holoparasite that subsists on the roots of plants and causes significant damage to the culture of leguminous plants and, in particular, to peas (Pisum sativum L.). Here, we investigated the potential of Rhizobium strains for biological control of Orobanche crenata using a commercial pea cultivar (Douce de province) and different Rhizobium strains. Firstly, benefit of bacterial inoculation on plant growth and efficiency in N-incorporation were demonstrated with four isolates, P.SOM, P.1001, P.Mat.95 and P.1236. After five Rhizobium strains (three efficient: P.SOM, P.1236, P.Mat.95 and two not efficient: P.OM1.92, P.MleTem.92) were investigated for their ability to control Orobanche crenata using pot and Petri dish experiments. Inoculation of peas with two (P.SOM and P.1236) of the five strains induced a significant decrease in O. crenata seed germination and in the number of tubercles on pea roots. Furthermore, other symptoms, including the non-penetration of the germinated seeds into pea roots followed by radicle browning and death of the parasites, were observed in the presence of these inoculated pea plants. The hypothesis that roots secrete toxic compounds related to Rhizobium inoculation is discussed.

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“…One important aspect of competition experiments, especially those performed under natural conditions is (Vincent 1970) The numbers between parentheses indicate the number of samples in which each species occurred (Tas et al 1996) and other markers, occasionally including monoclonal antibodies (Aouani et al 1997), are used for this purpose. One advantage of PCR-based identification over the use of previously cited methods is that PCR is applied on DNA which is stable, while antibiotic resistance and antibodies markers are unstable and are influenced by environmental conditions.…”

Section: Inoculation Trials With the Local Strain M Ciceri Cmg6mentioning

confidence: 99%

Competition for nodule formation between introduced strains of Mesorhizobium ciceri and the native populations of rhizobia nodulating chickpea (Cicer arietinum) in Tunisia

Romdhane

Tajini

Trabelsi

et al. 2007

World J Microbiol Biotechnol

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Chickpea is the most cultivated grain legume in the world and it shares the first rank with faba bean in Tunisia. However, the yield remains low, mainly due to the limited availability of N and P, and to the severe bioclimatic conditions. No inoculation trials had been conducted on chickpea in the Tunisian soils. This paper reports the yield response to inoculation by two different strains of Mesorhizobium ciceri, an exogenous type strain (UPMCa7 T ) and a selected local strain (CMG6). Field experiments were conducted in different sites in the north of Tunisia using three chickpea cultivars (cvs. Amdoun I, Chetoui and Kasseb). Rhizobia occupying field nodules were isolated and identified using 16S rDNA typing for both inoculated and non-inoculated plots. In contrast to the exogenous strain, the local strain gave a significant increase in nodule number and shoot dry yield in all the experimental fields for the three cultivars used. Monitoring of the nodule occupancy showed that the local strain competed well the native populations of rhizobia. The usefulness and the persistence of this strain in the different soils where it was introduced will be assessed further during the next years.

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Potential for inoculation of common bean by effective rhizobia in Tunisian soils

Cited by 21 publications

References 11 publications

Symbiotic effectiveness and response to mannitol-mediated osmotic stress of various chickpea–rhizobia associations

Symbiotic effectiveness and response to mannitol-mediated osmotic stress of various chickpea–rhizobia associations

The potential of Rhizobium strains for biological control of Orobanche crenata

Competition for nodule formation between introduced strains of Mesorhizobium ciceri and the native populations of rhizobia nodulating chickpea (Cicer arietinum) in Tunisia

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