Potential G-quadruplex forming sequences and<i>N</i><sup>6</sup>-methyladenosine colocalize at human pre-mRNA intron splice sites

Jara-Espejo, Manuel; Fleming, Aaron M.; Burrows, Cynthia J.

doi:10.1101/2020.02.07.939116

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…The m 6 A modification regulates various aspects of RNA metabolism, including splicing, translation efficiency, nuclear export, stability, and translation [82]. Bioinformatics analyses have revealed significant overlap and functional synergy between G4 structures and m 6 A-modified sites [83,84]. Although G4s can provide a framework for m 6 A modification, the latter can destabilize the G4 structure [85].…”

Section: Factors Involved In G4-mediated Biological Functionsmentioning

confidence: 99%

Crosstalk between G-quadruplex and ROS

Jiang

et al. 2023

Cell Death Dis

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The excessive production of reactive oxygen species (ROS) can lead to single nucleic acid base damage, DNA strand breakage, inter- and intra-strand cross-linking of nucleic acids, and protein-DNA cross-linking involved in the pathogenesis of cancer, neurodegenerative diseases, and aging. G-quadruplex (G4) is a stacked nucleic acid structure that is ubiquitous across regulatory regions of multiple genes. Abnormal formation and destruction of G4s due to multiple factors, including cations, helicases, transcription factors (TFs), G4-binding proteins, and epigenetic modifications, affect gene replication, transcription, translation, and epigenetic regulation. Due to the lower redox potential of G-rich sequences and unique structural characteristics, G4s are highly susceptible to oxidative damage. Additionally, the formation, stability, and biological regulatory role of G4s are affected by ROS. G4s are involved in regulating gene transcription, translation, and telomere length maintenance, and are therefore key players in age-related degeneration. Furthermore, G4s also mediate the antioxidant process by forming stress granules and activating Nrf2, which is suggestive of their involvement in developing ROS-related diseases. In this review, we have summarized the crosstalk between ROS and G4s, and the possible regulatory mechanisms through which G4s play roles in aging and age-related diseases.

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Section: Factors Involved In G4-mediated Biological Functionsmentioning

confidence: 99%

Crosstalk between G-quadruplex and ROS

Jiang

et al. 2023

Cell Death Dis

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“…Our findings contribute to an emerging picture of an interplay between RNA G-quadruplex formation and post-transcriptional modification of RNA. 25,26 ■ RESULTS Inosine Substitutes for Guanosine in RNA G-Quadruplexes In Vitro. To study the properties of putative GI-quadruplexes, we used a well-characterized model sequence comprising three G-quartets (G3 wt ).…”

Section: ■ Introductionmentioning

confidence: 99%

Inosine Substitutions in RNA Activate Latent G-Quadruplexes

et al. 2021

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It is well-accepted that gene expression is heavily influenced by RNA structure. For instance, stem-loops and Gquadruplexes (rG4s) are dynamic motifs in mRNAs that influence gene expression. Adenosine-to-inosine (A-to-I) editing is a common chemical modification of RNA which introduces a nucleobase that is iso-structural with guanine, thereby changing RNA base-pairing properties. Here, we provide biophysical, chemical, and biological evidence that A-to-I exchange can activate latent rG4s by filling incomplete G-quartets with inosine. We demonstrate the formation of inosine-containing rG4s (GIquadruplexes) in vitro and verify their activity in cells. GIquadruplexes adopt parallel topologies, stabilized by potassium ions. They exhibit moderately reduced thermal stability compared to conventional G-quadruplexes. To study inosine-induced structural changes in a naturally occurring RNA, we use a synthetic approach that enables site-specific inosine incorporation in long RNAs. In summary, RNA GI-quadruplexes are a previously unrecognized structural motif that may contribute to the regulation of gene expression in vivo.

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Potential G-quadruplex forming sequences andN⁶-methyladenosine colocalize at human pre-mRNA intron splice sites

Cited by 2 publications

References 23 publications

Crosstalk between G-quadruplex and ROS

Crosstalk between G-quadruplex and ROS

Inosine Substitutions in RNA Activate Latent G-Quadruplexes

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Potential G-quadruplex forming sequences andN6-methyladenosine colocalize at human pre-mRNA intron splice sites

Cited by 2 publications

References 23 publications

Crosstalk between G-quadruplex and ROS

Crosstalk between G-quadruplex and ROS

Inosine Substitutions in RNA Activate Latent G-Quadruplexes

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Potential G-quadruplex forming sequences andN⁶-methyladenosine colocalize at human pre-mRNA intron splice sites