Potential genotoxicity and risk assessment of a chlorinated flame retardant, Dechlorane Plus

Dou, Jing; Jin, Yuan; Li, Yajie; Wu, Bing; Li, Mei

doi:10.1016/j.chemosphere.2014.12.066

Cited by 23 publications

(10 citation statements)

References 34 publications

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“…Micronucleus test for Vicia faba was performed based on the reported method 19,20 . Randomly selected views on the slides were monitored to determine the number of micronucleated cells.…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of toxicity reduction of wastewater in twelve industrial park wastewater treatment plants based on battery of toxicity assays

Jiang

et al. 2019

Sci Rep

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Wastewater treatment plants (WWTPs) in industrial parks provide centralized treatment for industrial and domestic wastewater. However, the information on toxicity reduction of wastewater and its correlation with treatment process in industrial park is limited. This study compared the toxicity reduction of wastewater in 12 industrial park WWTPs based on battery of toxicity assays. Nine toxic endpoints involving microorganism, phytoplankton, zooplankton, plant and human cell lines were applied. All the influents of WWTPs induced high toxicities, which were significantly reduced after the treatments from 7 of the studied WWTPs. However, the effluents of five WWTPs induced higher toxicity in one or more toxic endpoints compared to the influents. This study also found that most of anaerobic-anoxic-oxic (A 2 /O)-based processes had good removal efficiency of wastewater toxicity, while the sequencing batch reactor (SBR)-based processes had the lowest removal efficiency. Moreover, low correlation coefficients were obtained among all toxic endpoints, indicating that battery of toxicity assays was necessary to completely characterize the toxicity and risk of wastewater in industrial parks. This study shed new lights to the toxicity reduction of wastewater and its correlation with treatment process, which is very useful for the design, management and operation of WWTPs in industrial parks.

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“…Micronucleus test for Vicia faba was performed based on the reported method 19,20 . Randomly selected views on the slides were monitored to determine the number of micronucleated cells.…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of toxicity reduction of wastewater in twelve industrial park wastewater treatment plants based on battery of toxicity assays

Jiang

et al. 2019

Sci Rep

Self Cite

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“…This protozoan species is widely used in genetic studies due to its well characterized genome [21,56,57]. Tetrahymena has been validated as a model organism for the evaluation of DNA damage by a modified comet assay protocol using well known mutagens such as phenol, hydrogen peroxide, and formaldehyde, which exhibited [62] as well as melamine, a raw material used in the chemical industry [63]. Altogether, these results indicate that the comet assay employing Tetrahymena may be used as a cost-effective and reliable tool for genotoxicity assessments.…”

Section: Protozoansmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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“…DP can also induce spontaneous movement of zebrafish embryos, with significantly reduced free style speed and significantly reduced larval swimming speed (Chen et al 2017). DP can also induce DNA damage in Tetrahymena thermophila and marine bivalves ((Dou et al 2015;Baron et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Toxic effects of dechlorane plus on the common carp (Cyprinus carpio) embryonic development

Chen

et al. 2020

Chemosphere

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Dechlorane Plus (DP) is a widely using chlorinated flame retardant, and has been detected extensively in the environment. Even though the DP content in the surface water is low, it can pose a continuous exposure risk to aquatic organisms due to its strong bioaccumulation. Since related researches on the toxicity mechanism of DP exposure on carp embryo development are limited, we carried out the evaluation of DP effect on carp. In this study, Carp embryos were exposed to DP of 0, 30, 60, and 120 µg/L at 3h post fertilization (hpf). The expression of genes associated with the neural and skeletal development such as sox2, sox19a, Mef2c, and BMP4 were evaluated using quantitative PCR (qPCR), and analysis of different developmental toxicity endpoints changes were observed with microscopy. Our results demonstrated that the expression of sox2, sox19a, Mef2c, and BMP4 were significantly changed and several developmental defects were observed in the exposed carps embryo, such as DNA damage, increased mortality rate, delayed hatching time, decreased hatching rate, decreased body length, increased morphological deformities. In addition, reactive oxygen species (ROS) and malondialdehyde (MDA) were significantly increased with DP exposure at 60µg/L and 120 µg/L. Altogether, our results suggest that DP may have an unique modes of action, which results in aberration occurrence in the early development stage of common carps, that may relates to some gene damage and oxidative stress. Besides, the parameters evaluated here can be used as tools to access the environmental risk for biota and humans exposed to DP.

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Potential genotoxicity and risk assessment of a chlorinated flame retardant, Dechlorane Plus

Cited by 23 publications

References 34 publications

Comparative analysis of toxicity reduction of wastewater in twelve industrial park wastewater treatment plants based on battery of toxicity assays

Comparative analysis of toxicity reduction of wastewater in twelve industrial park wastewater treatment plants based on battery of toxicity assays

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Toxic effects of dechlorane plus on the common carp (Cyprinus carpio) embryonic development

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