Potential group B Streptococcus interspecies transmission between cattle and people in Colombian dairy farms

Cobo-Ángel, Claudia; Jaramillo-Jaramillo, Ana S.; Palacio-Aguilera, Monica; Jurado-Vargas, Liliana; Calvo-Villegas, Edwin A.; Ospina-Loaiza, Diego A.; Rodríguez-Lecompte, J. C.; Sánchez, Javier; Zadoks, Ruth N.; Ceballos-Márquez, Alejandro

doi:10.1038/s41598-019-50225-w

Cited by 28 publications

(39 citation statements)

References 53 publications

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“…S. agalactiae has been considered an extremely contagious mastitis pathogen for several years, and an important human pathogen. It was reported that some clonal complexes of S. agalactiae were shared between cows and farm personnel, indicating the zoonotic potential of this species ( Cobo-Angel et al, 2019 ). These pathogens are also producers of biofilms ( Gomes et al, 2016 ; Reinoso, 2017 ; Alves-Barroco et al, 2019 ; Horiuk et al, 2019 ), and this virulence characteristic has been associated with persistent infections and development of antibiotic resistance ( Olson et al, 2002 ; Ruppen et al, 2017 ).…”

Section: Clinical and Veterinary Relevance Of Pyogenic Biofilmmentioning

confidence: 99%

Singularities of Pyogenic Streptococcal Biofilms – From Formation to Health Implication

et al. 2020

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Biofilms are generally defined as communities of cells involved in a self-produced extracellular matrix adhered to a surface. In biofilms, the bacteria are less sensitive to host defense mechanisms and antimicrobial agents, due to multiple strategies, that involve modulation of gene expression, controlled metabolic rate, intercellular communication, composition, and 3D architecture of the extracellular matrix. These factors play a key role in streptococci pathogenesis, contributing to therapy failure and promoting persistent infections. The species of the pyogenic group together with Streptococcus pneumoniae are the major pathogens belonging the genus Streptococcus, and its biofilm growth has been investigated, but insights in the genetic origin of biofilm formation are limited. This review summarizes pyogenic streptococci biofilms with details on constitution, formation, and virulence factors associated with formation.

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Section: Clinical and Veterinary Relevance Of Pyogenic Biofilmmentioning

confidence: 99%

Singularities of Pyogenic Streptococcal Biofilms – From Formation to Health Implication

et al. 2020

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“…Recently, GBS associated antimicrobial resistance has also emerged. Clindamycin‐resistant strains have caused more than 40 % of GBS infections, limiting prevention and treatment options for people with severe penicillin allergy in the US, and a human GBS ST1 isolate showed a surprisingly high penicillin resistance in Colombia …”

Section: Introductionmentioning

confidence: 99%

Structure‐Guided Design of a Group B Streptococcus Type III Synthetic Glycan–Conjugate Vaccine

Oldrini

Bino

Ardá

et al. 2020

Chemistry A European J

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Identification of glycan functional epitopes is of paramounti mportance for rational design of glycoconjugate vaccines. We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), am ajor cause of invasive disease in newborns, by using ad imer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with aprotectivemAb. Althoughreported data hadsuggested ah ighly complex epitope contained in ah elical structure composed of more than four repeating units, we showed that such dimer conjugated to ac arrierp rotein with a proper glycosylationd egree elicited functional antibodies comparably to the full-length conjugated polysaccharide.Here, startingf rom the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized ah exasaccharide comprising exclusively the relevant positions involved in binding. Combining competitive surface plasmon resonance and saturation transfer differenceN MR spectroscopy as well as in-silicom odeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.T he hexasaccharide conjugatedt oC RM 197 , am utant of diphtheria toxin, elicited ar obustf unctional immune response that was not inferior to the polysaccharide conjugate, indicating that it may sufficeasavaccine antigen. This is the first evidence of an X-ray crystallography-guided design of as ynthetic carbohydrate-basedc onjugatev accine. Supporting information and the ORCID identification number(s) for the author(s) of this articlecan be found under: https://doi.Figure 3. Immunogenicity of the synthetic hexasaccharide conjugate. A) Scheme of hexasaccharide conjugation to CRM 197 .B)Mouse immunization schedule. C) APSIII IgG Geometric Mean titers( GMTs, 95 %C I) aftert he second and third vaccineinjectionw ith CRM 197 -hexasaccharidea nd the CRM 197 -PSIII control. Dots indicateindividual mice.S tatistical analysiswas performed with Kruskal-Wallis and Dunn multiple comparisons test. D) Anti PSIII IgM Geometric Mean Titers after second immunization elicited by CRM 197 -hexasaccharide and the control CRM 197 -PSIII. E) OPKA titers measured after the third immunization.Animal studies were authorized by the Italian Ministry Of Health and were undertaken in accordance with the regulations of the Directive 2010/63/EU. Full experimental details can be found in the Supporting Information.

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“…Such comparisons, however, are not necessary for evolutionary analysis, whereby host species jumps have commonly been inferred based on sequence data of isolates derived from different host species without known interactions or epidemiological relatedness [19,61,62]. For the emergence of GBS in farmed species, several routes of transmission from humans to animals can be envisaged, including, in the case of fishes, the use of human waste for nutrient recycling and, in the case of cattle, the handling and milking of cows, which may lead to direct human-to-animal transmission [6,58,41]. Changes in animal husbandry systems combined with pathogen evolution are the likely explanation for the re-emergence of GBS, which has been observed in several countries in Europe [2,18,63].…”

Section: Concluding Commentsmentioning

confidence: 99%

The fall and rise of group BStreptococcusin dairy cattle: reintroduction due to human-to-cattle host jumps?

Crestani

Forde

Lycett

et al. 2021

Preprint

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Group B Streptococcus (GBS; Streptococcus agalactiae) is a major neonatal and opportunistic bacterial pathogen of humans and an important cause of mastitis in dairy cattle with significant impacts on food security. Following the introduction of mastitis control programs in the 1950s, GBS was nearly eradicated from the dairy industry in northern Europe, followed by re-emergence in the 21st century. Here, we sought to explain this re-emergence based on short and long read sequencing of historical (1953-1978; n = 44) and contemporary (1997-2012; n = 76) bovine GBS isolates. Our data show that a globally distributed bovine-associated lineage of GBS was commonly detected among historical isolates but never among contemporary isolates. By contrast, tetracycline resistance, which is present in all major GBS clones adapted to humans, was commonly and uniquely detected in contemporary bovine isolates. These observations provide evidence for strain replacement and suggest a human origin of newly emerged strains. Three novel GBS plasmids were identified, including two showing >98% homology with plasmids from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis, which co-exist with GBS in the human oropharynx. Our findings support introduction of GBS into the dairy population due to human to-cattle jumps on multiple occasions and demonstrate that reverse zoonotic transmission can erase successes of animal disease control campaigns.IMPACT STATEMENTPathogens can jump between humans and animals. Animal domestication and intensification of livestock production systems have caused multiple human to animal spill-over events, sometimes with significant impact on animal health and food production. The most common production-limiting disease of dairy cattle is mastitis, inflammation of the mammary gland, which can be caused by group B Streptococcus, a common commensal and pathogen of humans. Using genomic data from historical and recent isolates, we show that re-emergence of this pathogen in the dairy industry in northern Europe is due to strains with genomic signatures of human host-adaptation, including antimicrobial resistance genes and plasmids. This shows how elimination of animal diseases may be hampered by humans serving as a reservoir of multi-host pathogens, and reverse zoonotic transmission.REPOSITORIESReads for all isolates sequenced in this study have been submitted to the ENA Sequence Read Archive (SRA). SRA accession numbers are included in Table S1, supplementary material, available in the online version of this article.

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Potential group B Streptococcus interspecies transmission between cattle and people in Colombian dairy farms

Cited by 28 publications

References 53 publications

Singularities of Pyogenic Streptococcal Biofilms – From Formation to Health Implication

Singularities of Pyogenic Streptococcal Biofilms – From Formation to Health Implication

Structure‐Guided Design of a Group B Streptococcus Type III Synthetic Glycan–Conjugate Vaccine

The fall and rise of group BStreptococcusin dairy cattle: reintroduction due to human-to-cattle host jumps?

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