Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses

Gunny, Ahmad Anas Nagoor; Arbain, Dachyar; Gumba, Rizo Edwin; Jong, Bor Chyan; Jamal, Parveen

doi:10.1016/j.biortech.2013.12.101

Cited by 81 publications

(27 citation statements)

References 29 publications

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“…isolated from the Brazilian sponges Amphimedon viridis and Dragmacidon reticulata have also been reported to be good producers of laccases [29]. With this in mind we focused our attention on the ligninolytic ability of the three fungal strains Cadophora , Emericellopsis and Pseudogymnoascus , particularly given that ligninolytic enzymes from marine-derived fungi are likely to find utility in biotechnological applications with alkaline pH, high salinity, low temperature, oligotrophic conditions, low water potential and hydrostatic pressure, such as amongst others- the treatment of coloured industrial effluent and bioremediation in high salt concentration environments, biofuel production, deinking, laundry and in the food industry [11,27,101]. …”

Section: Resultsmentioning

confidence: 99%

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

et al. 2017

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Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less information is available on fungal biodiversity associated with these sponges. Following screening of fourteen fungi isolated from the deep-sea sponge Stelletta normani sampled at a depth of 751 metres, three halotolerant strains (TS2, TS11 and TS12) were identified which displayed high CMCase and xylanase activities. Molecular based taxonomic approaches identified these strains as Cadophora sp. TS2, Emericellopsis sp. TS11 and Pseudogymnoascus sp. TS 12. These three fungi displayed psychrotolerance and halotolerant growth on CMC and xylan as sole carbon sources, with optimal growth rates at 20°C. They produced CMCase and xylanase activities, which displayed optimal temperature and pH values of between 50–70°C and pH 5–8 respectively, together with good thermostability and halotolerance. In solid-state fermentations TS2, TS11 and TS12 produced CMCases, xylanases and peroxidase/phenol oxidases when grown on corn stover and wheat straw. This is the first time that CMCase, xylanase and peroxidase/phenol oxidase activities have been reported in these three fungal genera isolated from a marine sponge. Given the biochemical characteristics of these ligninolytic enzymes it is likely that they may prove useful in future biomass conversion strategies involving lignocellulosic materials.

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Section: Resultsmentioning

confidence: 99%

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

et al. 2017

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“…The obtained halotolerance was found slightly higher than that of the cellulases of Aspergillus terreus UniMAP AA‐6 (Gunny et al . ). The mechanism of the halotolerance remained uncertain, but a few hypotheses have been suggested, such as acidic amino acids on cellulases, which can provide more charge on the enzyme surface and inhibit aggregation upon contact with salts (Xu et al .…”

Section: Discussionmentioning

confidence: 97%

An isolatedAmycolatopsissp. GDS for cellulase and xylanase production using agricultural waste biomass

Kshirsagar

Saratale

et al. 2015

J Appl Microbiol

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“…This is noteworthy because of its potential for transformation of lignocellulose to glucose in a single‐step process. In other work, Gunny et al . showed that halophile cellulases produced from Aspergillus terrus exhibited very high tolerance to ILs and enhanced thermostability, displaying a 95% filter paper activity (FPase) in 10% [Emim]Ac at 30 °C, and more than 55% FPase activity at 70 °C.…”

Section: Bioenergy‐producing Biorefineriesmentioning

confidence: 99%

An overview of the enzyme potential in bioenergy-producing biorefineries

Escamilla‐Alvarado

Perez-Pimienta

Ponce-Noyola

et al. 2016

J. Chem. Technol. Biotechnol.

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Biorefineries are considered as an integrative thinking that focuses on the possibility of obtaining as many added-value products as technically and economically feasible. However, in practice most biorefineries comprise only enzymatic or chemical pretreatment followed by biofuel generation. The drop in oil prices may menace the development of this young industry, as has happened before in history. This has become a fundamental reason for which the biofuel industry should not consider only biofuels production, but enzyme and non-fuel based chemicals as well. Hence, this work aims at overviewing the most important enzymes involved in biotechnological processes and to describe their role in biorefineries. Bioethanol, biogas and biodiesel biorefineries are overviewed, along with the integrated and industrial types. Finally separation and purification processes in biorefineries are discussed.

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Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses

Cited by 81 publications

References 29 publications

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

An isolatedAmycolatopsissp. GDS for cellulase and xylanase production using agricultural waste biomass

An overview of the enzyme potential in bioenergy-producing biorefineries

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