Potential impact of WTAP and YTHDF2 on tumor immunity in lung adenocarcinoma

Zhang, Xinyu; Cai, Xinsheng

doi:10.1097/md.0000000000031195

Cited by 2 publications

(1 citation statement)

References 46 publications

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“…In fact, WTAP is widely expressed in many types of tumors and is involved in numerous biological processes. The high expression of WTAP is significantly associated with poor prognosis in gastric cancer patients 31 . Besides, WTAP acts as a tumor suppressor gene by methylating 3'-UTR of CAV-1 and then activating NF-κB signaling pathway in endometrial cancer 32 .…”

Section: Discussionmentioning

confidence: 99%

PIWI-interacting RNA-17458 is oncogenic and a potential therapeutic target in cervical cancer

Liu,

Li,

et al. 2023

J. Cancer

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Cervical cancer (CC) is one of the leading cancers among the female reproductive system. The piwi-interacting RNA (piRNA) function and biogenesis has been studied in various cancers, including CC. But the precise mechanism of piRNA in CC is still unknown. In our study, we found that piRNA-17458 was overexpressed in CC tissues and cells. piRNA-17458 mimic and inhibitor promoted and suppressed proliferation, migration and invasion ability of CC cells, respectively. We also demonstrated that piRNA-17458 mimic could contribute to tumor growth in mice xenograft models. Besides, we also found that the piRNA-17458 mimic could enhance mRNA N6-methyladenosine(m6A) levels and increase WTAP stability in CC cells, while the effects of the mimic was reversed by the WTAP knockdown. The results of dual luciferase reporter assay showed that WTAP was a direct target of piRNA-17458. Knockdown of WTAP attenuated proliferation, migration and invasion of CC cells in piRNA-17458 mimic group. Our finding not only demonstrates for the first time that piRNA-17458 is overexpressed in CC tissues and cells, but also shows that piRNA-17458 promotes tumorigenesis of CC in a WTAP-mediated m6A methylation manner.

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Section: Discussionmentioning

confidence: 99%

PIWI-interacting RNA-17458 is oncogenic and a potential therapeutic target in cervical cancer

Liu,

Li,

et al. 2023

J. Cancer

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Wilms’ Tumor 1-Associating Protein Promotes Nonsmall-Cell Lung Cancer Through the Expression of Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5

Liu,

Gao,

Yang

et al. 2024

American Journal of Clinical Oncology

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Objectives: This study aimed to analyze the functional roles and molecular mechanism of Wilms’ tumor 1-associating protein (WTAP) in the tumorigenesis of nonsmall-cell lung cancer (NSCLC). Methods: Retrospective analysis was used. Tumor tissues and surrounding nontumor tissues of 150 patients with NSCLS who were surgically resected in the Fourth Hospital of Hebei Medical University from January 2016 to January 2018 were selected. The expression of WTAP in NSCLC tissues was detected by immunohistochemistry. Clinicopathologic parameters were then subjected to univariate and multivariate Cox regression analysis in purpose of uncovering the independent risk factors for overall survival time. MTS (3-[4,5-dimethylthiazol-zyl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazoliuzolium, inner salt) assay, colony formation assay, and transwell assays were performed to estimate cell proliferation, migration, and invasion. Meanwhile, the relationship between WTAP and the cell migration and invasion marker-related proteins were evaluated by Western blot analysis and RT-qPCR. WTAP expression was knocked-down in cell lines by shRNA, and RNA-Seq was performed to investigate the pathways regulated by WTAP. Results: In NSCLC patients, WTAP was highly expressed in tumor tissues and the higher expression was significantly associated with poor overall survival (OS) (P<0.01). Compared with the control group in vitro, the overexpression of WTAP could significantly promote cell proliferation, migration, and invasion (P<0.01), while knock-down WTAP significantly reduces the above effects (P<0.01). In a mouse orthotopic implantation model, higher WTAP abundance could significantly promote tumor enlargement compared with the control group (P<0.01). Compared with the control group, the knock-down of WTAP significantly inhibit the expression of carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) in cell lines (P<0.01). Besides, in NSCLC, knocked-down CEACAM5 significantly reduced the impact of WTAP on cell proliferation, migration, and invasion compared with the control group (P<0.05). Conclusions: This study suggests that high expression of WTAP was associated with poor clinical outcomes. CEACAM5 may play a synergistic role with WTAP to jointly promote NSCLC progression by enhancing cell proliferation, invasion, and migration.

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Potential impact of WTAP and YTHDF2 on tumor immunity in lung adenocarcinoma

Cited by 2 publications

References 46 publications

PIWI-interacting RNA-17458 is oncogenic and a potential therapeutic target in cervical cancer

PIWI-interacting RNA-17458 is oncogenic and a potential therapeutic target in cervical cancer

Wilms’ Tumor 1-Associating Protein Promotes Nonsmall-Cell Lung Cancer Through the Expression of Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5

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