ynthesis and evaluation of in vitro antioxidant potential of silver nanoparticles (AgNPs) made from methanolic leaf extract of Cassia siamea, To develop a herbal, potential, cost-effective source of antioxidative agents.Herbal matter have been a good source of nutrition and antioxidant agents from the ages, to fulfil the requirements of one by its natural phytoconstituents. Present study compiles the, green synthesis of AgNPs using the methanolic leaf extract of C. siamea. Synthesized AgNPs were characterized and used for the detection of their in vitro antioxidant potential. The activity was analysed through DPPH and FRAP assays. While the antioxidant potential of AgNPs is known, the use of C. siamea extract for this purpose is relatively underexplored, making this study a significant contribution to the field of nanotechnology and natural antioxidants. The findings highlight the potential of plant-mediated AgNPs for future applications in biomedicine, particularly as natural, eco-friendly antioxidants.The collection and identification of the plant leaf was done in University Campus. The AgNPs were characterized through several techniques as UV-Vis spectrophotometry, FTIR, XRD, particle size and Zeta potential analysis, SEM, confirming the formation of stable AgNPs. The in vitro antioxidant potential of the synthesized AgNPs was evaluated using DPPH and FRAP assays, with ascorbic acid serving as the reference standard. By current study this can be concluded that after successful synthesis and characterization of AgNPs, DPPH Assay demonstrated that AgNPs had a concentration-dependent % scavenging action, with considerable radical inhibition at all examined levels, but they are lower than ascorbic acid. Similarly, FRAP assay demonstrated the reducing power of AgNPs, which increased with concentration. The IC values obtained from both assays indicate that synthesized AgNPs possess substantial antioxidant activity.The bioactive chemicals in the leaf extract play an important role in nanoparticle formation and their stability. These findings suggest that green-synthesized AgNPs from Cassia siamea exhibit considerable antioxidant potential, making them promising candidates for applications in biomedicine and nanotechnology. This study contributes to the growing body of research on plant-mediated nanoparticle synthesis and the potential of such nanoparticles as natural antioxidants.
