Potential Infectious Hazards of Laboratory Techniques I

Laboratory-Associated Infections: Incidence, Fatalities, Causes, and Prevention

“…Photography (76) and air sampling (4,55,130,146,147,193) have shown that removing the lid from a Waring Blendor, removing the closure of a shaken culture, plunging a loop into a flame, and numerous other procedures may release migroorganisms into the environment. Lyo philization, animal inoculation, and egg inoculation and harvesting have also been shown to produce aerosols (143)(144)(145). It is little wonder, then, that infections have occurred in persons who were known only to have worked with the agent involved.…”

Potential Infectious Hazards of Laboratory Techniques Ii

“…The methods of sampling and preparation of lyophilized material are described elsewhere (Reitman et al, 1954). The following menstruums were used: (a) nutrient broth, (b) one part nutrient broth plus one part skim milk, (C) sheep serum inactivated at 56 C for one-half hour, (d) Naylor's solution,2 and (e) skim milk plus glycerin (10 per cent milk solids with 2 per cent glycerin).…”

Microbiological safety

“…niger, designated B. globigii in Fort Detrick laboratories; and (c) coliphage T3. S. indica and B. subtilis spores were sampled on cornsteep, molasses agar, and coliphage T3 on tryptose phosphate glucose agar (7).…”