Potential Intervention of Campylobacter jejuni in the Modulation of Murine Immune Response

Pancorbo, Pedro L.; Pablo, Manuel A. de; Ortega, Elena; Puertollano, M.a A.; Gallego, Aurelia M.; Cienfuegos, Gerardo Álvarez de

doi:10.1007/s002840010289

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…C. jejuni invades epithelial cells in vitro [3], and can be isolated from the spleen and liver in infected animals [4,5]. In vitro studies demonstrated the production of proinflammatory cytokines in C. jejuni -infected monocytic [6], dendritic [7] and intestinal epithelial cell lines [8], peripheral blood mononuclear cells [9] and splenocytes [10]. The association between cytokine production and disease protection/resolution, suggested in patients [9], was shown by the increased susceptibility of mice deficient in MyD88 or NF-κB to C. jejuni [11,12].…”

Section: Resultsmentioning

confidence: 99%

Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice

Al-Banna

Albert

2012

Gut Pathog

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BackgroundCytokine production and histopathological changes occur in the lungs of mice after intranasal inoculation with Campylobacter jejuni, but the levels of cytokines in different organs to which C. jejuni disseminates have not been studied.FindingsAdult BALB/c mice were intranasally inoculated with C. jejuni 81–176 (test) or phosphate-buffered saline (control) (n=16 per group). The levels of cytokines in the organs (spleen, liver, and small and large intestines) to which C. jejuni disseminated were measured by ELISA. Two cytokine patterns were observed. First, increased proinflammatory cytokines, TNF-α, IL-1, and IL-2, were followed by anti-inflammatory cytokines, IL-4 and IL-10 in the spleen and large intestine. Second, in the liver and small intestine, there was a predominant production of anti-inflammatory cytokines, IL-4 and IL-10, with some increase in IL-2 levels. In the spleen and intestines, the levels of pro- and anti-inflammatory cytokines were concurrently increased.ConclusionDissemination of C. jejuni is associated with the production of different cytokine profiles in different tissues, with the proinflammatory response appearing in the spleen and large intestine at an earlier time point than in the liver and small intestine. The organs produce different cytokine profiles in response to C. jejuni dissemination. These preliminary findings should be confirmed with a study involving a larger group of animals.

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Section: Resultsmentioning

confidence: 99%

Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice

Al-Banna

Albert

2012

Gut Pathog

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“…Therefore, it is not unlikely that the production of IL-6 is a mechanism that perpetuates the inflammatory response to C. jejuni infection. Previous studies have showed that an enterotoxigenic strain of C. jejuni could induce the production of IL-2, while it lacked the ability to stimulate the production of macrophage-derived cytokines 43 . Similarly, TNF-α, IL-1β, IL-6, and IL-8 were also found to be the major components of the immune response to orogastric infection with other species 39 44 .…”

Section: Discussionmentioning

confidence: 99%

Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model

Shang

Ren

Song

et al. 2016

Sci Rep

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A lack of relevant disease models for Campylobacter jejuni has long been an obstacle to research into this common enteric pathogen. Here we used an infant rabbit to study C. jejuni infection, which enables us to define several previously unknown but key features of the organism. C. jejuni is capable of systemic invasion in the rabbit, and developed a diarrhea symptom that mimicked that observed in many human campylobacteriosis. The large intestine was the most consistently colonized site and produced intestinal inflammation, where specific cytokines were induced. Genes preferentially expressed during C. jejuni infection were screened, and acs, cj1385, cj0259 seem to be responsible for C. jejuni invasion. Our results demonstrates that the infant rabbit can be used as an alternative experimental model for the study of diarrheagenic Campylobacter species and will be useful in exploring the pathogenesis of other related pathogens.

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“…Previous studies have indicated that an enterotoxigenic strain of C. jejuni could stimulate the production of IL-2, while it lacked the ability to induce the production of macrophage-derived cytokines (38,39). C. jejuni 81-176 could induce significantly higher levels of IL-2 in the lungs of infected mice (on day 1) (Fig.…”

Section: Mouse Lung Model Of Infectionmentioning

confidence: 99%

Correlation of Proinflammatory and Anti-Inflammatory Cytokine Levels with Histopathological Changes in an Adult Mouse Lung Model ofCampylobacter jejuniInfection

Al-Banna

Albert

2008

Clin Vaccine Immunol

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Campylobacter jejuni is a major cause of diarrhea in humans. A mouse lung model of infection was previously established for C. jejuni. We used this model to study cytokine production in the lungs and correlated it with pathological changes. C. jejuni strain 81-176 or sterile phosphate-buffered saline was intranasally inoculated into adult BALB/c mice. The levels of proinflammatory cytokines (gamma interferon, tumor necrosis factor alpha, interleukin-1␤ [IL-1␤], IL-2) and anti-inflammatory cytokines (IL-4, IL-10), in addition to those of IL-6, were assessed on days 1, 3, and 5 postinfection by enzyme-linked immunosorbent assay, and the ratios of proinflammatory cytokines to anti-inflammatory cytokines were calculated. Since IL-6 is unique in that it is both a proinflammatory cytokine and a TH2 cytokine, it was considered to be both in the determination of these ratios. The significance of the cytokine levels and ratios were determined by the Mann-Whitney U test (P < 0.05). The induction of proinflammatory cytokines in the lungs of infected mice, as indicated by the cytokine levels and ratios, coincided with the accumulation of neutrophils and activated macrophages, in addition to the clearance of the bacterial load and bacteriumlike structures that we have previously shown in the same groups of mice. This was followed by increased levels of anti-inflammatory cytokines and the resolution of inflammation and pathology in the lungs. This study demonstrates the dynamics of cytokine production and their correlation with tissue inflammation and the resolution of infection. This model is useful for further studies of the pathogenesis of C. jejuni infection and vaccine evaluation.

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Potential Intervention of Campylobacter jejuni in the Modulation of Murine Immune Response

Cited by 5 publications

References 21 publications

Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice

Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice

Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model

Correlation of Proinflammatory and Anti-Inflammatory Cytokine Levels with Histopathological Changes in an Adult Mouse Lung Model ofCampylobacter jejuniInfection

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