Potential matrix metalloproteinase-9 inhibitor of aurone compound isolated from Sterculia quadrifida leaves: In-vitro and in-silico studies

Abstract

In our previous study, aurone compound were found in the n-butanol fraction of Sterculia quadrifida leaves. The aurone compound (compound 1) had high cytotoxic activity in cell lines with IC50 values of 4.05, 12.53, 15.38, and 25.91 μg/mL in 4T1, MCF7, MDA-MB-435, T47D, respectively. In this study, we performed the MMP-9 enzyme inhibition test with FRET assay, and to deepen the mechanism of action of the compounds use molecular docking and molecular dynamics. The results showed that compound 1 had the MMP-9 enzyme inhibition of 91.23% and IC50 48.27%. Furthermore, molecular docking and molecular dynamic simulation suggest compound 1 interacts firmly and stably at the enzyme catalytic site. In conclusion, this study can provide important information on the development of herbal medicines that are potentially cancer drugs.

The effect of concentration and storage time of faloak (Sterculia quadrifida R. Br) bark extract on the ability to inhibit Escherichia coli that cause mastitis

“…Several studies show that compounds from the Malvaceae family have antibacterial activity [12]. The results of the in vitro antibacterial test of faloak bark extract against Escherichia coli bacteria are presented in Table 1 Five percent concentration of faloak bark extract has the smallest inhibitory diameter and 50% concentration of faloak bark extract has the largest inhibitory diameter.…”

The Evaluation of the Acute Toxicity Of MCH2 Syrup Formulations on the Liver Organs of Male Wistar Strain Rats Entailed Histopathological Observations and the Examination of Symptoms of Toxicity

“…6 The transition of traditional medicines into Standardized Herbal Medicines necessitates pre-clinical testing and raw material standardization. 7 Despite their natural origin, herbal medicines are not inherently completely safe, necessitating assessments of safety, including toxicity tests. 8 © 2023 the authors.…”

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In this study, an acute toxicity test of the MCH2 syrup, comprising P. niruri herb and M. citrifolia fruit extract in a 2:2 ratio, was conducted to ascertain intrinsic toxicity, identify target organs, assess species sensitivity, and determine initial dose levels, providing essential hazard information post-acute exposure. 9 The test involved repeated oral administration of the substance to observe toxicity symptoms, with subsequent histopathological examination focusing on the liver, a pivotal organ in detoxification. 10 The study aimed to contribute insights into identifying toxic chemicals and associated hazards by evaluating symptoms of toxicity in test animals.

Materials and Methods

MaterialsThe study utilized the following materials: P. niruri, M. citrifolia, purified water, methyl paraben, sucrose, sorbitol, sodium benzoate, tutty fruity flavor, rattus norvegicus male rat, diethyl ether, buffer neutral formalin 10%.

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In-vitro and In-silico Studies of a Phenylpropanoid Compound Isolated from Sterculia quadrifida Seeds and Its Inhibitory Effect on Matrix Metalloproteinase-9

“…Notably, (2E,4E)-1,5diphenylpenta-2,4-dien-1-one did not show toxicity towards Vero cells (normal cells), as indicated by an SI value greater than 2. 6 Of significance is the high cytotoxicity observed in 4T1 cells, which represent triple-negative breast cancer. Consequently, the potent cytotoxic activity of (2E,4E)-1,5-diphenylpenta-2,4-dien-1-one in 4T1 cells suggests its potential for further investigation as an inhibitor of MMP-9 enzymes.…”