Potential metabolic strategies of widely distributed holobionts in the oceanic archipelago of St Peter and St Paul (Brazil)

Rua, Cintia P. J.; Gregoracci, Gustavo Bueno; Santos, Eidy de Oliveira; Soares, Ana Carolina; Francini‐Filho, Ronaldo B.; Thompson, Fabiano L.

doi:10.1093/femsec/fiv043

Cited by 23 publications

(29 citation statements)

References 71 publications

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“…The symbionts extend the metabolic capabilities of the host by mediating processes, such as photosynthesis (Wilkinson, 1983;Arillo et al, 1993;Steindler et al, 2002), carbon (De Goeij et al, 2008a,b;de Goeij et al, 2013), nitrogen (Wilkinson and Fay, 2004;Mohamed et al, 2008;Southwell et al, 2008a;Hoffmann et al, 2009;Schläppy et al, 2010;Fiore et al, 2013), methane (Vacelet et al, 1995), sulfur (Hoffmann et al, 2005), and phosphorus (Sabarathnam et al, 2010;Zhang et al, 2015b) cycling. Metagenomic and transcriptomic studies of sponge microbiomes have provided independent molecular evidence of the contribution of symbiotic bacteria, in particular, to biogeochemical and nutrient cycling Fiore et al, 2015;Rua et al, 2015), underscoring the significance of the holobiont perspective in understanding ecological roles of animal hosts in marine ecosystems.…”

Section: Introductionmentioning

confidence: 99%

“…More broadly, recent metatranscriptomic, and metaproteomic analyses of sponge-associated microbial consortia have begun to unveil their global expressed gene repertoire Radax et al, 2012;Moitinho-Silva et al, 2013Fiore et al, 2015;Rua et al, 2015). Single-cell genomic and metagenomics studies have contributed to correlating particular traits with specific symbiont lineages Siegl et al, 2011;Kamke et al, 2013;Gao et al, 2014;Tian et al, 2014;Wilson et al, 2014;Burgsdorf et al, 2015;Britstein et al, 2016), revealing functional gene convergence in phylogenetically-distinct microbial communities with core features that likely reflect adaptation of microorganisms to the sponge host environment Hentschel et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Draft Genomes Shed Light on the Dual Bacterial Symbiosis that Dominates the Microbiome of the Coral Reef Sponge Amphimedon queenslandica

2016

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Amphimedon queenslandica is a coral reef demosponge that houses a low complexity and low abundance microbiota dominated by a proteobacterial duo for which draft genomes are presented here. The most prevalent symbiont, AqS1, is a sulfur-oxidizing gammaproteobacterium closely related to other demosponge symbionts and to free-living Ectothiorhodospiraceae (Chromatiales). The predicted gene repertoire of AqS1 indicates that it is capable of sulfur oxidation, carbon monoxide oxidation and inorganic phosphate assimilation, and that some of its metabolic capabilities may have been acquired via horizontal gene transfer from alphaproteobacteria. The second most prevalent symbiont, AqS2, is a betaproteobacterium whose closest known relatives are other demosponge symbionts. AqS1 has characteristic sponge symbiont features, including a versatile nutrient use with large number of transporters, ankyrin-repeat-containing proteins, and a CRISPR system. Based on the size of its genome assembly, AqS2 is predicted to have a much smaller genome with many fewer symbiotic features than AqS1. The smaller is reflected in its more limited metabolic capabilities that include carbohydrate metabolism, but not sulfur oxidation or phosphorus metabolism. Within-pathway complementation and resource partitioning potentially occur between the two bacteria. The addition of these symbiont genomes to extensive genome and transcriptome resources already available for the sponge host now permits the development of mixed-species genome-scale metabolic models as a foundation for experimental investigations of resource partitioning between symbionts and host.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Draft Genomes Shed Light on the Dual Bacterial Symbiosis that Dominates the Microbiome of the Coral Reef Sponge Amphimedon queenslandica

2016

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“…Various differentially abundant ASVs in the tetillid sponges were affiliated with taxa known to be involved in the nitrogen cycle. Nitrifying prokaryotes that transform ammonia to nitrite, such as Nitrosopumilaceae (Li et al, 2014;Feng et al, 2016), the AqS1 group of Nitrosococcaceae (Rua et al, 2015;Feng & Li, 2019), and the SAR202 clade of phylum Chloroflexi (Morris et al, 2004;Mincer et al, 2007), were detected in the tetillids. Members of Nitrospira, some of which are enriched in Paratetilla sp., may contribute to the conversion of nitrite into nitrate (Hentschel et al, 2002;Daims & Wagner, 2018).…”

Section: Predicted Functions Of Tetillid-associated Prokaryotesmentioning

confidence: 99%

Population structure and microbial community diversity of two common tetillid sponges in a tropical reef lagoon

Baquiran

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et al. 2020

PeerJ

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Sponges are predicted to dominate future reef ecosystems influenced by anthropogenic stressors and global climate change. The ecological success of sponges is attributed to their complex physiology, which is in part due to the diversity of their associated prokaryotic microbiome. However, the lack of information on the microbial community of many sponge species makes it difficult to gauge their interactions and functional contributions to the ecosystem. Here, we investigated the population dynamics and microbial community composition of two tetillid sponges identified as Cinachyrella sp. and Paratetilla sp., which are common on coral bommies in a reef lagoon in Bolinao, northwestern Philippines. The sponges ranged in size from 2.75 ± 2.11 to 6.33 ± 3.98 cm (mean ± standard deviation) and were found at an average density of 1.57 ± 0.79 to 4.46 ± 3.60 individuals per sq. m. on the bommies. The tetillid sponge population structure remained stable over the course of four years of monitoring. Prokaryotic communities associated with the sponges were distinct but had overlapping functions based on PICRUSt2 predictions. This convergence of functions may reflect enrichment of metabolic processes that are crucial for the survival of the tetillid sponges under prevailing conditions in the reef lagoon. Differentially enriched functions related to carbon, sulfur, fatty acid, and amino acid metabolism, cellular defense, and stress response, may influence the interactions of tetillid sponges with other biota on the bommies.

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“…As linhagens bacterianas frequentemente abundantes em esponjas incluem membros dos filos Proteobacteria, Chloroflexi, Actinobacteria, Acidobacteria, Firmicutes, Cyanobacteria e o candidato a filo Poribacteria (WEBSTER et al, 2001;, SCHIMTT et al, 2012REVEILLAUD et al, 2014;RUA et al, 2015;THOMAS et al, 2016). Entretanto, é importante destacar que comunidades raras no ambiente marinho podem também rapidamente tornar-se abundantes ou desempenhar funções importantes para o ecossistema (LYNCH;NEUFELD, 2015;FUHRMAN et al, 2015 (HENTSCHEL et al, 2002;REVEILLAUD et al, 2014).…”

Section: Associação Bactéria-unclassified

Exploração da diversidade bacteriana de esponjas marinhas por abordagens dependente e independente de cultivo

Souza¹

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Bioprospecting the bacterial diversity of marine sponges by culture-dependent and culture-independent approaches This study describes the diversity of associated bacterial communities to five marine sponges, and the potential of these microorganisms as producers of bioactive substances with fungicidal properties. Sponges live in symbiosis with microorganisms that have a high ecological interest, evolutionary and biotechnological. However, this microbial system remains poorly understood. To fully understand sponge biology, it is necessary to describe the ecological and evolutionary factors that influence the structure and dynamics of their microbial communities. In this work, it is supported the hypothesis that the taxonomic composition and structure of bacterial communities correlate with phylogenetic relatedness of their corresponding hosts. Bacterial communities associated with the sponges Aplysina fulva, Aiolochroia crassa, Chondrosia collectrix, Didiscus oxeata and Scopalina ruetzleri were examined using the Ion Torrent platform for partial sequencing of the 16S rRNA gene. Seawater surrounding specimens were collected for comparisons. The analysis detected a complex and specific microbial system living in sponges, with the operational taxonomic units dominant classified in the phyla: Acidobacteria, Actinobacteria, Chloroflexi, Proteobacteria and Gemmatimonadetes. Despite sympatric occurrence of the specimens, the studied sponges presented different bacterial compositions that differed from those observed in seawater. However, lower dissimilarities in bacterial communities were clearly observed within sponges from the same phylogenetic group (A. fulva and A. crassa). Isolation of bacteria was done from the sponges D. oxeata and S. ruetzleri. Fifty-six strains were isolated and classified into three phyla: Actinobacteria, Proteobacteria and Firmicutes. Phylogenetic analysis indicated five possible novel bacterial species. Based in a polyphasic taxonomy approach, one of the isolates denominated ASPSP 40 was identified as belonging to a novel species of the genus Saccharopolyspora for which the name, Saccharopolyspora spongiae sp. nov. has been proposed. All bacterial isolates were evaluated by their antagonisms against Pythium species. Two of them, Terrabacter sp. ASPSP 140 and Bacillus sp. ASPSP 434 demonstrated strong potential in inhibiting the following species P. aphanidermatum, P. ultimum and P. graminicola. The bioactive secondary metabolites of both, characterized by LC-MS/MS, were identified as a mixture of cyclic dipepitides belonging to the class of diketopiperazine (DKP). This is the first report of fungicidal activity, and thus the detection of DKP of the genus Terrabacter.

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Potential metabolic strategies of widely distributed holobionts in the oceanic archipelago of St Peter and St Paul (Brazil)

Cited by 23 publications

References 71 publications

Draft Genomes Shed Light on the Dual Bacterial Symbiosis that Dominates the Microbiome of the Coral Reef Sponge Amphimedon queenslandica

Draft Genomes Shed Light on the Dual Bacterial Symbiosis that Dominates the Microbiome of the Coral Reef Sponge Amphimedon queenslandica

Population structure and microbial community diversity of two common tetillid sponges in a tropical reef lagoon

Exploração da diversidade bacteriana de esponjas marinhas por abordagens dependente e independente de cultivo

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