POTENTIAL MICROALGA Chlorella vulgaris FOR BIOREMEDIATION OF HEAVY METAL Pb

Abstract

This research was conducted from April to July 2019 at the Center for Environmental Technology Laboratory (PTL), Geostech 820 Building, Serpong Region Puspitek, South Tangerang. The aim of this research was to determin growth of Chlorella vulgaris on media added with Pb at different concentrations, and to determine the ability to absorb Pb. The experimental method was conducted by using concentrations of Pb at 3 different levels consisting of 1 ppm, 5 ppm, 10 ppm in triplicate and control treatment without the addition of Pb. Each sample was analyzed by ICP-OES (Inductivly Coupled Plasma – Optical Emission Spectrometer). Data was analyzed by using ANOVA followed by LSD test. The growth of C. vulgaris biomass during the cultivation were Pb 1 ppm (10.38 g / l), k (9.10 g / l), Pb 5 ppm (8.36 g/l) and Pb 10 ppm (7.13) g/l). ANOVA test showed that different concentrations of Pb gave a very significant difference (Sig. <0.05) on the growth of C. vulgaris. Reduction in the concentration of Pb metal in culture media were Pb 10 ppm (96.8%), Pb 5 ppm (96.2%), Pb 1 ppm (90%) and there is no Pb found in control. ANOVA test results showed that C. vulgaris had a very significant effect (Sig. <0.05) on the decrease of Pb metal concentration. This shows that C. vulgaris has the capacity as bioremediation of Pb with different concentrations.

Phycoremediation Using Microalgae Chlorella vulgaris and Nannochloropsis oculata to Remove Lead Heavy Metals

“…The graph in Figure 1 shows 4 phases in microalgae growth, namely: lag phase, exponential phase, stationary phase, and death phase. In the Chlorella vulgaris microalgae, days 0 to 2 show a lag phase where the microalgae adapt and the addition of cells looks little or even tends to be absent (Halima et al, 2019). Day 3 shows the exponential phase until the peak of growth on day 7, days 8 to 10 is the stationary phase, which is characterized by a stable number of cells and no cell addition (Kurniawan & Aunurohim, 2014).…”

Produksi dan uji aktivitas enzim fatty acid photodecarboxylase dari Chlorella variabilis melalui kultivasi pada medium CYT dan KW21

“…Selanjutnya, fase kematian pada H23 diindikasi oleh penurunan jumlah sel yang cepat melalui pengecekan OD di medium KW21 menjadi 0,536 dan di medium CYT menjadi 1,884. Kematian sel mikroalga disebabkan oleh kandungan nutrien dalam medium semakin terbatas serta perubahan kualitas air ke arah yang buruk akibat dari kemampuan metabolisme mikroalga yang semakin menurun (Halima et al, 2020).…”

Bioremediasi Logam Berat Pb(II) Dan Cu(II) Pada Air Lindi Menggunakan Chlorella Vulgaris

“…Pada Bak control, pada H0 (awal) sampai hari ke 14 nilai pH rata-rata 7, merupakan nilai normal (Putra & Fitri, 2019). Nilai pH ini sesuai untuk media hidup Cv, pH optimum untuk pertumbuhan Cv adalah 6-7 (Halima, Effendi, & Ambarsari, 2019) , (Regaldo, Gervasio, Troiani, & Gagneten, 2013). Pada Bak Lindi terjadi peningkatan pH dari H0 sampai hari ke 14 dan mengalami penurunan pada hari ke 14 dan 21.…”

“…Dari hasil peneltian terlihat bahwa parameter kulitas Air yang umumnya cukup optimum. Komunitas mikroalga pada suatu perairan dipengaruhi oleh kondisi lingkungan antara lain temperature/suhu, intensitas cahaya, derajat keasaman (pH), aerasi sumber O 2 (Halima, Nursyirwani, Effendi, & Ambarsar, 2020)…”

“…Logam berat dapat mengahambat pertumbuhan pada beberapa algae hijau karena logam berat dapat menurunkan kandungan protein, karbohidrat, pigmen fotosintesis, Proses metabolisme nitrat serta respirasi. Pembelahan Cv yang terhambat ini dapat disebabkan oleh pergantian pada struktur membran tilakoid kloroplas akibat adanya Logam berat, sehingga terjadi penghambatan fotosintesis (Halima et al, 2019). Meskipun demikian, Cv tetap dapat tumbuh dengan baik pada medium lindi.…”