Potential neuroprotection of protodioscin against cerebral ischemia-reperfusion injury in rats through intervening inflammation and apoptosis

Zhang, Xinxin; Xue, Xuanji; Xian, Liang; Guo, Zengjun; Ito, Yoichiro; Sun, Wen-Ji

doi:10.1016/j.steroids.2016.06.008

Cited by 39 publications

(16 citation statements)

References 41 publications

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“…Triterpenoid saponins have a wide range of pharmacological properties . These kinds of compounds have also been reported to have detrimental effects on plant growth when released into the soil .…”

Section: Discussionmentioning

confidence: 99%

Chemical evidence for the effect of Urochloa ruziziensis on glyphosate‐resistant soybeans

Nepomuceno

Chinchilla

Varela

et al. 2017

Pest Management Science

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Section: Discussionmentioning

confidence: 99%

Chemical evidence for the effect of Urochloa ruziziensis on glyphosate‐resistant soybeans

Nepomuceno

Chinchilla

Varela

et al. 2017

Pest Management Science

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“…Male Sprague‐Dawley rats, weighing 300‐320 g, were obtained from Beijing Vital River Laboratory Animal Co. (Beijing, China). The transient focal cerebral I/R injury was induced using the MCAO method by the intraluminal suture as described previously . Briefly, the rats were anaesthetized with intraperitoneal injection of chloral hydrate (350 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

Limb remote ischaemic postconditioning‐induced elevation of fibulin‐5 confers neuroprotection to rats with cerebral ischaemia/reperfusion injury: Activation of the AKT pathway

Zhang

Wang

2017

Clin Exp Pharma Physio

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Limb remote ischaemic postconditioning (RIPostC) is an effective and well-acknowledged treatment for brain ischaemia injury. The present study aimed to evaluate the role of fibulin-5 in the neuroprotection of RIPostC against cerebral ischaemia/reperfusion (I/R) injury in rats. The middle cerebral artery occlusion (MCAO) model was established in rats and then RIPostC was carried out by three cycles of 10 minutes occlusion/10 minutes release of the bilateral femoral artery at the beginning of the reperfusion. To downregulate the fibulin-5 level, fibulin-5 siRNA was injected into the lateral ventricle 24 hours before MCAO. According to our present study, RIPostC attenuated cerebral I/R injury by decreasing infarct volume, improving neurobehavioral score and suppressing blood brain barrier (BBB) leakage. Moreover, the mRNA and protein levels of fibulin-5 were upregulated by RIPostC at 24 hours and 72 hours after reperfusion. Downregulation of fibulin-5 attenuated the neuroprotection of RIPostC. Finally, the result showed that fibulin-5 was upregulated by RIPostC via activation of the PI3K/AKT pathway. Taken together, these results provide evidence that upregulation of fibulin-5 is involved in the beneficial effect of RIPostC against cerebral I/R injury.

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“…Dioscoreae rhizome is not only an important food in China, but according to traditional Chinese medicine, the Dioscoreae rhizome is good for the stomach and spleen, and it has been shown that PD significantly improves glucose intolerance, metabolic syndrome, and renal injury [4]. PD also revealed potential neuroprotection against the ischemia-reperfusion injury [5]. In addition, PD exhibits growth inhibition on HL-60 cells by inducing apoptosis [6] and anti-cancer effects in several types of cancer cells [7].…”

Section: Introductionmentioning

confidence: 99%

Protodioscin Induces Apoptosis Through ROS-Mediated Endoplasmic Reticulum Stress via the JNK/p38 Activation Pathways in Human Cervical Cancer Cells

Lin

Lee

Chen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Protodioscin (PD) is a steroidal saponin with anti-cancer effects on a number of cancer cells, but the anti-tumor effects and mechanism of action of PD on human cervical cancer cells is unclear. Methods: We determined cell viability using the MTT assay. Cell death, mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) generation, and endoplasmic reticulum (ER) stress were measured on a flow cytometry. Caspase activation, ER stress, and MMP-dependent apoptosis proteins in cervical cancer cells in response to PD were determined by Western blot analysis. The ability of ATF4 binding to ChIP promoter was measured using the ChIP assay. Results: We demonstrated that PD inhibits cell viability, causes a loss of mitochondrial function, and induces apoptosis, as evidenced by up-regulation of caspase-8, -3, -9, -PARP, and Bax activation, and down-regulation of Bcl-2 expression. PD was shown to induce ROS and the ER stress pathway, including GRP78, p-eIF-2α, ATF4, and CHOP. Pre-treatment with NAC, a ROS production inhibitor, significantly reduced ER stress and apoptosis-related proteins induced by PD. Transfection of GRP78/CHOP-siRNA effectively inhibited PD-induced ER stress-dependent apoptosis. Moreover, treatment with PD significantly increased p38 and JNK activation. Co-administration of a JNK inhibitor (SP600125) or p38 inhibitor (SB203580) abolished cell death and ER stress effects during PD treatment. In addition, PD induced the expression of nuclear ATF4 and CHOP, as well as the binding ability of ATF4 to the CHOP promoter. Conclusion: Our results suggest that PD is a promising therapeutic agent for the treatment of human cervical cancer.

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Potential neuroprotection of protodioscin against cerebral ischemia-reperfusion injury in rats through intervening inflammation and apoptosis

Cited by 39 publications

References 41 publications

Chemical evidence for the effect of Urochloa ruziziensis on glyphosate‐resistant soybeans

Chemical evidence for the effect of Urochloa ruziziensis on glyphosate‐resistant soybeans

Limb remote ischaemic postconditioning‐induced elevation of fibulin‐5 confers neuroprotection to rats with cerebral ischaemia/reperfusion injury: Activation of the AKT pathway

Protodioscin Induces Apoptosis Through ROS-Mediated Endoplasmic Reticulum Stress via the JNK/p38 Activation Pathways in Human Cervical Cancer Cells

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