Potential of a Penicillium echinulatum enzymatic complex produced in either submerged or solid-state cultures for enzymatic hydrolysis of elephant grass

Menegol, Daiane; Scholl, A.; Fontana, Roselei Claudete; Dillon, Aldo José Pinheiro; Camassola, Marli

doi:10.1016/j.fuel.2014.05.003

Cited by 41 publications

(32 citation statements)

References 37 publications

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“…The chemical composition of elephant grass was characterized according to the National Renewable Energy Laboratory methods (NREL-TP-510-42618, NREL-TP-510-42619, NREL-TP-510-42621) with the adaptations described by (Menegol et al, 2014a). For this, elephant grass samples were extracted with water and ethanol to remove low molar mass extractable components prior to sulphuric acid hydrolysis to quantify glucans (mostly cellulose), hemicelluloses (mostly acetylated heteroxylans) and both acid soluble and acid insoluble lignins.…”

Section: Characterization Of Elephant Grass Before and After Pretreatmentioning

confidence: 99%

“…Enzymatic hydrolysis was carried out according to (Menegol et al, 2014a) using various steam-exploded elephant grass samples (unwashed and water-washed). Untreated elephant grass, Celuflock Ò cellulose (Cotia, São Paulo, Brazil) and Avicel PH101 (Fluka, Buchs, Ireland) were used as controls.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

“…Therefore, together with sugarcane bagasse, elephant grass is a http://dx.doi.org/10.1016/j.biortech.2015.05.065 0960-8524/Ó 2015 Elsevier Ltd. All rights reserved. good option for cellulosic ethanol and for several other high value-added biotechnological applications (Menegol et al, 2014a). The increased photosynthetic efficiency of elephant grass is attributed to its C4 metabolism (Euclides et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Scholl

Menegol

Pitarelo

et al. 2015

Bioresource Technology

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In this work, steam explosion was used a pretreatment method to improve the conversion of elephant grass (Pennisetum purpureum) to cellulosic ethanol. This way, enzymatic hydrolysis of vaccum-drained and water-washed steam-treated substrates was carried out with Penicillium echinulatum enzymes while Saccharomyces cerevisiae CAT-1 was used for fermentation. After 48 h of hydrolysis, the highest yield of reducing sugars was obtained from vaccum-drained steam-treated substrates that were produced after 10 min at 200 °C (863.42 ± 62.52 mg/g). However, the highest glucose yield was derived from water-washed steam-treated substrates that were produced after 10 min at 190 °C (248.34 ± 6.27 mg/g) and 200 °C (246.00 ± 9.60 mg/g). Nevertheless, the highest ethanol production was obtained from water-washed steam-treated substrates that were produced after 6 min at 200 °C. These data revealed that water washing is a critical step for ethanol production from steam-treated elephant grass and that pretreatment generates a great deal of water soluble inhibitory compounds for hydrolysis and fermentation, which were partly characterized as part of this study.

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Section: Characterization Of Elephant Grass Before and After Pretreatmentioning

confidence: 99%

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Scholl

Menegol

Pitarelo

et al. 2015

Bioresource Technology

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“…Pennisetum purpureum, also known as elephant grass, belongs to the family Graminae (Poaceae) [1]. It is a tropical species presenting C4 metabolism and high productivity, yielding approximately 45 t of dry matter/ha/year, whereas sugar cane and corn yield approximately 21 t (sugar and bagasse) and 13 t (grain and stover), respectively [2].…”

Section: Introductionmentioning

confidence: 99%

Different elephant grass ( Pennisetum purpureum ) accessions as substrates for enzyme production for the hydrolysis of lignocellulosic materials

Basso

Machado

Lédo

et al. 2014

Biomass and Bioenergy

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“…O capim elefante tem sido analisado como potencial fonte de biomassa para produção de etanol por poucos autores, cujas pesquisas mostram a necessidade de otimizar as condições de operação para melhorar a eficiência da produção de etanol, especialmente na etapa de pré-tratamento da biomassa (Usberti et al, 1988;Cardona et al, 2013;Menegol et al, 2014). Vários tipos de pré-tratamento têm sido usados com a finalidade de alterar ou remover a lignina, aumentar a área superficial e diminuir o grau de polimerização e cristalinidade da celulose visando facilitar a digestão enzimática (Canilha et al, 2010).…”

Section: Introductionunclassified

Potencialidade Do Capim Elefante Para a Produção De Etanol De Segunda Geração

Muniz¹,

Berdet²,

Silva³

2015

Anais Do Congresso Brasileiro De Engenharia Química Em Iniciação Científica - Cobeq IC 2015

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RESUMO -Com o aquecimento global, decorrente da emissão de gases do efeito estufa, principalmente o CO 2 gerado na queima de combustíveis fósseis, políticas mundiais têm apontado para a necessidade de produção de combustíveis utilizando produtos da fotossíntese. Uma nova fonte alternativa de matéria-prima para a produção de etanol é o capim elefante (Pennisetum purpureum), que é uma gramínea de grande porte, fácil manejo e cultivo e abundante em todo o Brasil. Este trabalho teve como objetivo, determinar a potencialidade do capim elefante, colhido pela Embrapa Pecuária Sul (CPPSul) no município de Bagé no estado do Rio Grande do Sul, para a produção de álcool de segunda geração. Para isso, a biomassa foi caracterizada por análise granulométrica, apresentando diâmetro médio de Sauter de 0,432 mm e, quimicamente, pelo método de Van Soest, que mostrou que a fração de capim elefante com maior potencial para a produção de etanol é a de folhas jovens com 22,01 % de hemicelulose, 52,28 % de celulose e 8,28 % de lignina.Foram testados três tipos de pré-tratamento: ácido, hidrotérmico e básico, seguidos de hidrólise enzimática com a enzima comercial Celluclast® 1,5 L. O melhor resultado encontrado pelo método de 3,5-dinitrosalicilico (DNS) foi 1,94 g/L de açúcares redutores obtido com pré-tratamento alcalino usando hidróxido de sódio 0,25 M. Esse resultado também foi encontrado em análise morfológica preliminar por microscopia óptica.

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Potential of a Penicillium echinulatum enzymatic complex produced in either submerged or solid-state cultures for enzymatic hydrolysis of elephant grass

Cited by 41 publications

References 37 publications

Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Ethanol production from sugars obtained during enzymatic hydrolysis of elephant grass (Pennisetum purpureum, Schum.) pretreated by steam explosion

Different elephant grass ( Pennisetum purpureum ) accessions as substrates for enzyme production for the hydrolysis of lignocellulosic materials

Potencialidade Do Capim Elefante Para a Produção De Etanol De Segunda Geração

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