Potential of a Pycnoporus sanguineus laccase in bioremediation of wastewater and kinetic activation in the presence of an anthraquinonic acid dye

Trovaslet, Marie; Enaud, Estelle; Guiavarc’h, Yann; Corbisier, Anne-Marie; Vanhulle, Sophie

doi:10.1016/j.enzmictec.2007.03.007

Cited by 73 publications

(55 citation statements)

References 54 publications

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“…18,19) In addition to physiological functions, due to their broad substrate specificity, laccases have the unique ability to oxidize a broad range of xenobiotic compounds, such as synthetic dyes, 20) pesticides, 21) and chlorinated phenolics. 22) Hence, they have attracted considerable interest for application in many fields of industrial and environmental processes, including paper pulping and bleaching, 23,24) textile dye bleaching, 25) bioremediation, 26) and effluent detoxification. 27) The potential applications of laccases in food processing have also received much attention.…”

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confidence: 99%

Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom,Grifola frondosa: Purification and Characterization

Nitheranont

Watanabe

Asada

2011

Bioscience, Biotechnology, and Biochemistry

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confidence: 99%

Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom,Grifola frondosa: Purification and Characterization

Nitheranont

Watanabe

Asada

2011

Bioscience, Biotechnology, and Biochemistry

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“…A capacidade de produção de lacase por fungos do gênero Pycnoporus é conhecida (TROSVALET et al, 2007;FONSECA et al, 2010). Entretanto, a produção dessa enzima em meios contendo fontes alternativas e mais baratas de nutrientes ainda é pouco conhecida e explorada.…”

Section: Discussionunclassified

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

Fabrini

Avelino

Marim

et al. 2017

ArqVet

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Umuarama, v. 19, n. 3, p. 159-164, jul./set. 2016. RESUMO:Lacases são polifenol oxidases que utilizam a capacidade redox de íons cobre para reduzir oxigênio a água e oxidar um substrato fenólico. A síntese e secreção de lacases de basidiomicetos dependem de vários fatores como os nutrientes presentes no meio de cultura. Visando à produção de lacase, Pycnoporus sanguineus foi cultivado em meio contendo melaço de soja como única fonte de carbono, ureia como fonte de nitrogênio suplementar em diferentes concentrações (0,6; 1,2; 2,4; 4,8 e 9,6 g/L de nitrogênio) e diferentes concentrações de CuSO 4 (0, 150, 200, 250 e 300 µM). O extrato enzimático produzido nas melhores condições de cultivo foi utilizado para a descoloração dos corantes remazol azul brilhante R (antraquinona), amarelo 145, preto 5, vermelho 195 (azo) e verde malaquita (trifenilmetano). As concentrações de nitrogênio não afetaram a produção de lacase, exceto a maior concentração (9,6 g/L) que reduziu a atividade enzimática. A adição de cobre ao meio de cultivo (150 µM) aumentou a atividade de lacase em 112%. A maior atividade de lacase (~34300 U/L) promoveu a descoloração dos corantes remazol azul brilhante R (67,5%) e verde malaquita (28,3%) em 24h, sendo os corantes azo descoloridos apenas parcialmente. Concluiu-se que o melaço de soja é um resíduo agroindustrial adequado para produção de lacase de P. sanguineus com potencial para degradação de corantes. PALAVRAS-CHAVE: Antraquinona. Corante azo. Ligninases. Poliporaceae. Resíduos agroindustriais. LACCASE PRODUCTION BY Pycnoporus sanguineus IN CULTURE MEDIA WITH SOYBEAN MOLASSESABSTRACT: Laccases are multicopper oxidases using the redox ability from copper ions to reduce oxygen to water, while oxidizing a phenolic substrate. Laccase synthesis and secretion in basidiomycetes depend on the conditions provided and on the nutrients present in the culture medium. Pycnoporus sanguineus was cultivated in medium containing soybean molasses as the sole carbon source, with urea as the source of supplemental nitrogen at different concentrations (0.6, 1.2, 2.4, 4.8 and 9.6 g/L nitrogen), and different CuSO 4 concentrations (0, 150, 200, 250 and 300 μM). The enzymatic extract produced under the best cultivation conditions was used for the depigmentation of remazole brilliant blue R (anthraquinone), yellow 145, black 5, red 195 (azo) and malachite green (triphenylmethane). Nitrogen concentrations did not affect laccase production, except for the higher concentration (9.6 g/L) reducing enzymatic activity. The addition of copper to the culture medium (150 μM) increased laccase activity by 112%. The highest laccase activity (~34300 U/L) promoted the depigmentation of remazol brilliant blue R (67.5%) and malachite green (28.3%) dyes in 24 hours. Azo dyes were only partially discolored. Therefore, it can be considered that soybean molasses is an agro-industrial byproduct suitable for the production of P. sanguineus laccase with potential for dye degradation. KEYWORDS: Agro-industrial byproduct. Anthraquinone. Azo d...

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“…The toxicity of waste water containing dye varies with the type of dye used in the textile industry. [2] When the effluents are not properly managed, many pathogenic microorganisms and chemicals in the effluents may predispose the inhabitants to serious health hazards. It may alter the physicochemical parameters of soil or water bodies thereby affecting the ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Resistant Characterization of Dye-Adapted Bacteria From Tirupur Textile Effluent

Prabaharan¹

2017

WJPPS

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The textile Industries in Tirupur releases million litres of untreated effluents into the Noyyal River. The discharge of coloured compound into water bodies affect photosynthesis of aquatic plants and these dyes are carcinogenic in nature which is dangerous to human being. This Pseudomonas isolates were confirmed by 16S rRNA sequencing with universal bacterial primers (16S-UP-F, UP-R) and the sequences showed 100% similarity with the already established species P.aeruginosa which was deposited in GENBANK. The deposited 16S r DNA sequence of P.aeruginosa was given the accession number KU041528. The phylogenetic tree was constructed based on 16S r DNA sequence of P.aeruginosa. Multiple resistant activities of P. aeruginosa was detected through protease and Phospholipase test and

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Potential of a Pycnoporus sanguineus laccase in bioremediation of wastewater and kinetic activation in the presence of an anthraquinonic acid dye

Cited by 73 publications

References 54 publications

Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom,Grifola frondosa: Purification and Characterization

Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom,Grifola frondosa: Purification and Characterization

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

Isolation, Identification and Resistant Characterization of Dye-Adapted Bacteria From Tirupur Textile Effluent

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