Potential of Baccharis alnifolia Meyen & Walpan (Chilka) from northern Chile used as a medicinal infusion

Abstract

Baccharis alnifolia Meyen & Walp. is a native and medicinal, Chilean Altiplano herb that is used as a poultice and also consumed as an infusion to relieve inflammation. This study evaluated the antioxidant and cytotoxic effect of the ethanolic extract of B. alnifolia and their infusion. It was reported that the ethanol extract contains 4.42 mg GAE/g of dry weight and the infusion contains 35.86 mg GAE/L of total polyphenols. Also, it determined the antioxidant capacity using Ferric reducing antioxidant power (FRAP), Oxygen Radical Absorbance Capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) assay. Moreover, seven metabolites including between phenolic acids and flavonoids were identified for the first time in both extracts of the medicinal plant Baccharis alnifolia. The UHPLC-DAD chromatograms revealed the majority presence of ferulic acid and luteolin, both could be responsible for the antioxidant and cytotoxic activity. Furthermore, we realized a preliminary screening of cytotoxicity in different tumor cell lines, finding that these extracts have cytotoxic potential on kidney cells.

The Influence of Locality on Phenolic Profile and Antioxidant Capacity of Bud Extracts

“…The ABTS + assay was performed by bleaching the cationic radical ABTS + as described by Soto et al [ 16 ]. A solution of 7 mM ABTS + and 2.5 mM potassium persulfate was left to stabilize from 12 to 16 h in the dark at room temperature before use.…”

UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities

“…Liquid chromatography parameters were carried out as described by Soto et al [ 45 ], with some modifications. Analyzes were performed on a Knauer Azura analytical UHPLC system (Knauer, Berlin, Germany), consisting of Azura P 6.1 L pump, 3950 autosamplers, and Azura DAD 2.1 L diode array detector with high sensitivity Knauer.…”

Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS

“…The ABTS assay was performed by bleaching the cationic radical ABTS •+ as described by Soto et al [ 48 ]. For the preparation of the radical ABTS •+ , 2.5 mL of the 7 mM ABTS solution was mixed with 2.5 mL of 2.45 μM sodium persulfate for 12 h in the dark at 4 °C.…”