Potential of Bacillus amyloliquefaciens for Biocontrol of Bacterial Wilt of Tomato Incited by Ralstonia solanacearum

Singh, Dinesh; Yadav, D.; Chaudhary, Garima; Rana, Virendra Singh; Sharma, Raj Kumar

doi:10.4172/2157-7471.1000327

Cited by 49 publications

(43 citation statements)

References 34 publications

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“…The 1 mL of suspension then added 9 mL of sterile water and incubated at 80 o C for 30 min for Bacillus sp. target (Singh et al, 2016). Five times serial dillution then carried out, the fourth and fifth dilutions were cultured on the NA (nutrient agar) medium with spread plate method.…”

Section: Methodsmentioning

confidence: 99%

Screening of Competent Rice Root Endophytic Bacteria to Promote Rice Growth and Bacterial Leaf Blight Disease Control

Prihatiningsih

Djatmiko

Lestari

2020

Jurnal Hama dan Penyakit Tumbuhan Tropika

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Screening of competent rice root endophytic bacteria to promote rice growth and bacterial leaf blight disease control. This study was aimed to collect isolate endophytic bacterial of rice roots which able to produce IAA, determine the effect of endophytic bacteria application in stimulating rice plant growth, and evaluate the potential of rice root endophytic bacteria for controlling bacterial leaf blight. This reasearch was carried out at the Screen House, Plant Protection Laboratory, and Agrohorti Laboratory of the Agriculture Faculty, Jenderal Soedirman University. Isolation of rice root endophytic bacteria was carried out by purposive random sampling from several marginal lands. The results showed that 8 isolates of rice root endophytic bacteria were able to produce IAA, ranging from 57.56 to 79.33 ppm and B07 isolate from Serayu produced the highest amount of IAA. The B04 and B07 isolates were contributed to increase the rice plant growth. The application of rice root endophytic bacteria was effective in controlling bacterial leaf blight.

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Section: Methodsmentioning

confidence: 99%

Screening of Competent Rice Root Endophytic Bacteria to Promote Rice Growth and Bacterial Leaf Blight Disease Control

Prihatiningsih

Djatmiko

Lestari

2020

Jurnal Hama dan Penyakit Tumbuhan Tropika

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“…Although soil solarization has been applied for the control of numerous soil borne pathogens but its role for the management of bacterial wilt has not been much studied. However, the wilt incidence was slightly reduced by 8 weeks of soil solarization with 25 µm thick transparent polyethylene sheet (Sharma et al, 2004;Sharma et al, 2016).…”

Section: Cultural Practicesmentioning

confidence: 98%

“…Several biological control agents like Acinetobacter sp. (Xue et al, 2009), Burkholderia nodosa, B. sacchari, B. tericola, B. pyrrocinia (Nion and Toyota, 2008), bacteriophages (Yamada et al, 2007), Bacillus thuringiensis (Zhou et al, 2008), Bacillus subtilis (Singh et al, 2012a(Singh et al, , 2016, Bacillus sp. (Ramesh et al, 2009;Xue et al, 2009), Enterobacter sp.…”

Section: Biological Controlmentioning

confidence: 99%

Bacterial Wilt of Solanaceous Crops: Diagnosis, Diversity and Management

Singh¹

2017

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Wilt disease of solanaceous crops caused by bacterial pathogen Ralstonia solanacearum is a major devastating soil borne disease in the world, limiting the production of solanaceous vegetable crops, and affecting more than 450 plant species. The loss due to this disease is very high, ranging from 2 to 100% depending on environmental conditions and crops. The affected plants show a typical wilt symptoms and browning of vascular tissue in roots, stems and tubers. The pathogen is a gram-ve, rod shaped, frequently occurs in pairs, motile with 1 to 4 polar flagella, aerobic, non-fluorescent, positive in catalase and oxidase tests, forms nitrites from nitrates and negative in levan production as well as starch hydrolysis. Virulent isolates on 2,3,5-Triphenyl Tetrazolium Chloride (TTC) medium develop fluidal, irregular colonies having peripheral white and pinkish in centre. The optimum growth of the bacterium on artificial medium occurs at 28 to 32 o C. In India, race 1 and bvs 3 and 4 are prevalent in tomato chilli capsicum and brinjal. However, race 1 and 3 infect the potato. Presently, R. solanacearum strains are categorized into four genetic groups called phylotypes which reflect the geographical origin and also ancestral relationships between the strains. Besides cultural, morphological and biochemical tests, serodiagnostic like ELISA (Enzyme-linked immunosorbent assay), immunofluorescent cell staining, lateral flow devices, serological kits are applied for detecting R. solanacearum from infected plant, tubers and soil. For detecting R. solanacearum, polymerase chain reaction (PCR) based techniques are usually applied using amplification of ribosomal sequences (16S rRNA) and other conserved genes of bacteria. To improve the sensitivity, various modifications have been made like enrichment in SMSA broth, nutrient broth or CPG broth to allow bacteria to multiply prior to do conventional PCR, called as BIO-PCR and two round PCR as nested-PCR. Multiplex-PCR protocols have been developed to simultaneously detect either R. solanacearum and E. carotovora sub sp. carotovora or Clavibacter michiganensis sub sp. sepodonicus from tubers of potato and also R. solanacearum along with Xanthomonas perforans from tomato. Management of bacterial wilt disease of solanaceous crops is very difficult due to its soil borne nature. Different workers throughout the world have made efforts to control this disease by using cultural, chemical, biological and host resistance methods, either alone or in combination of these methods.

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“…In India, certain research work has been reported on the bio-control of bacterial wilt disease. The work published by Singh 22 , et. al.…”

Section: Introductionmentioning

confidence: 99%

Role of Rhizobacteria Associated with Diseased Tomato Plants towards their Response with Ralstonia sp The Bacterial Wilt Agent

et al. 2020

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In this study, we have aimed to determine the antagonistic effects of various rhizobacteria against representatives of bacterial wilt disease; Ralstonia pseudosolanacearum with a comparison from control sterile distilled water (SDW) aliquots. During in-vitro study, bacterial wilt agent was clearly inhibited in their growth by significant percentage by 32 different strains through perforated agar plate method. Results showed 10 potential strains among those 32 strains viz. DIB76/BCS-8, DIB76/BCS-9, DIB76/BCS-10, DIB76/BCS-12, DIB76/BCS-19, DIB76/BCS-20, DIB76/BCS-21, DIB76/BCS-24, DIB76/BCS-26 and DIB76/BCS-27 had the highest growth suppression for 9 different strains of R. pseudosolanacearum as compared to other bacterial strains. Trials with tomato crop at polyhouse had no incidence of bacterial wilt than those for control. Study, thus identifies 10 rhizobacterial strains from rhizosphere soil of diseased tomato plant possessing potential antagonistic activity against the wilt pathogen and has prospects as good biocontrol agents.

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Potential of Bacillus amyloliquefaciens for Biocontrol of Bacterial Wilt of Tomato Incited by Ralstonia solanacearum

Cited by 49 publications

References 34 publications

Screening of Competent Rice Root Endophytic Bacteria to Promote Rice Growth and Bacterial Leaf Blight Disease Control

Screening of Competent Rice Root Endophytic Bacteria to Promote Rice Growth and Bacterial Leaf Blight Disease Control

Bacterial Wilt of Solanaceous Crops: Diagnosis, Diversity and Management

Role of Rhizobacteria Associated with Diseased Tomato Plants towards their Response with Ralstonia sp The Bacterial Wilt Agent

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