Potential of Bacillus subtilis from marine environment to degrade aromatic hydrocarbons

Velupillaimani, Daisy; Muthaiyan, Arunachalam

doi:10.1007/s42398-019-00080-2

Cited by 9 publications

(4 citation statements)

References 52 publications

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“…In addition, no activities were observed for catechol C3-substitutions by –OH (pyrogallol) and –F (3-fluorocatechol) suggesting the preference for substrates with non-ionic substitutions. Also, the results showed a possible degradation of halocatechols by BLC23O at slightly higher activity against 4-chlorocatechol as compared to catechol 50 , 51 . However, since the specific cleavage product peak was not as obvious as other substrates, the detailed activity and cleavage product of 4-chlorocatechol need to be further confirmed.…”

Section: Resultsmentioning

confidence: 91%

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

Adewale

Lang

Huang

et al. 2021

Sci Rep

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Identification of novel enzymes from lignin degrading microorganisms will help to develop biotechnologies for biomass valorization and aromatic hydrocarbons degradation. Bacillus ligniniphilus L1 grows with alkaline lignin as the single carbon source and is a great candidate for ligninolytic enzyme identification. The first dioxygenase from strain L1 was heterologously expressed, purified, and characterized with an optimal temperature and pH of 32.5 °C and 7.4, respectively. It showed the highest activity with 3-ethylcatechol and significant activities with other substrates in the decreasing order of 3-ethylcatechol > 3-methylcatechol > 3-isopropyl catechol > 2, 3-dihydroxybiphenyl > 4-methylcatechol > catechol. It did not show activities against other tested substrates with similar structures. Most reported catechol 2,3-dioxygenases (C23Os) are Fe2+-dependent whereas Bacillus ligniniphilus catechol 2,3-dioxygenase (BLC23O) is more Mn2+- dependent. At 1 mM, Mn2+ led to 230-fold activity increase and Fe2+ led to 22-fold increase. Sequence comparison and phylogenetic analyses suggested that BL23O is different from other Mn-dependent enzymes and uniquely grouped with an uncharacterized vicinal oxygen chelate (VOC) family protein from Paenibacillus apiaries. Gel filtration analysis showed that BLC23O is a monomer under native condition. This is the first report of a C23O from Bacillus ligniniphilus L1 with unique substrate preference, metal-dependency, and monomeric structure.

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Section: Resultsmentioning

confidence: 91%

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

Adewale

Lang

Huang

et al. 2021

Sci Rep

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“…We also noticed changes in the prevalence of a few genes involved in functions that could be considered of bioprospecting interest for bioremediation applications, i.e. the genes nylB (K01453) and pcaG (K00448), respectively involved in the nylon degradation pathway [ 74 ] and in PAH degradation [ 75 ], which were acquired in skeleton samples from the acidified site.…”

Section: Resultsmentioning

confidence: 99%

Metagenomic shifts in mucus, tissue and skeleton of the coral Balanophyllia europaea living along a natural CO2 gradient

Palladino

Caroselli

Tavella

et al. 2022

ISME Communications

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Using the Mediterranean coral Balanophyllia europaea naturally growing along a pH gradient close to Panarea island (Italy) as a model, we explored the role of host-associated microbiomes in coral acclimatization to ocean acidification (OA). Coral samples were collected at three sites along the gradient, mimicking seawater conditions projected for 2100 under different IPCC (The Intergovernmental Panel on Climate Change) scenarios, and mucus, soft tissue and skeleton associated microbiomes were characterized by shotgun metagenomics. According to our findings, OA induced functional changes in the microbiomes genetic potential that could mitigate the sub-optimal environmental conditions at three levels: i. selection of bacteria genetically equipped with functions related to stress resistance; ii. shifts in microbial carbohydrate metabolism from energy production to maintenance of cell membranes and walls integrity; iii. gain of functions able to respond to variations in nitrogen needs at the holobiont level, such as genes devoted to organic nitrogen mobilization. We hence provided hypotheses about the functional role of the coral associated microbiome in favoring host acclimatation to OA, remarking on the importance of considering the crosstalk among all the components of the holobiont to unveil how and to what extent corals will maintain their functionality under forthcoming ocean conditions.

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“…Bacillus subtilis is a promising platform for drug production for several reasons. In addition to the inherent space tolerance of its spores (Horneck, 1993a), marine strains of this same host have also been shown to have an increased capacity for secondary metabolite secretion (Ivanova et al, 1999; Mondol et al, 2013; Pandey et al, 2014; Velupillaimani and Muthaiyan, 2019). This is thought to be a result of selective evolution in the highly competitive marine environment where secretion of toxic secondary metabolites as a defense mechanism is beneficial.…”

Section: Discussionmentioning

confidence: 99%

Bacillus subtilisengineered for aerospace medicine: a platform for off-planet production of pharmaceutical peptides

Vallota‐Eastman

Bui

Williams

et al. 2023

Preprint

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Biologics, such as pharmaceutical peptides, have notoriously short shelf lives, insufficient for long-duration space flight missions to the Moon or Mars. To enable the sustainable presence of humans on the Moon or Mars, we must develop methods for on-site production of pharmaceutical peptides in space, a concept we call Astropharmacy. Here, we present proof-of-concept for the first step needed: a low-mass system for pharmaceutical production designed to be stable in space. To demonstrate feasibility, we engineered strains of the space-hardy spore-forming bacterium, Bacillus subtilis, to secrete two pharmaceutical peptides important for astronaut health: teriparatide (an anabolic agent for combating osteoporosis) and filgrastim (an effective countermeasure for radiation-induced neutropenia). We found that the secretion peptides from the walM and yoqH genes of B. subtilis 168 worked well for secreting teriparatide and filgrastim, respectively. In consideration of the TRISH challenge to produce a dose equivalent in 24 hours, dried spores of our engineered strains were used to produce 1 dose equivalent of teriparatide from a 2 mL culture and 1 dose equivalent of filgrastim from 52 mL of culture in 24 hours. Further optimization of strain growth conditions, expression conditions, and promoter sequences should allow higher production rates to be achieved. These strains provide the template for future optimization efforts and address the first step in the Astropharmacy, capable of on-site production, purification, and processing of biopharmaceutical compounds in platforms amenable for use in space.

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Potential of Bacillus subtilis from marine environment to degrade aromatic hydrocarbons

Cited by 9 publications

References 52 publications

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement

Metagenomic shifts in mucus, tissue and skeleton of the coral Balanophyllia europaea living along a natural CO2 gradient

Bacillus subtilisengineered for aerospace medicine: a platform for off-planet production of pharmaceutical peptides

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