Potential of Bone Marrow Stromal Cells in Applications for Neuro-Degenerative, Neuro-Traumatic and Muscle Degenerative Diseases

Dezawa, Mari; Ishikawa, Hiroto; Hoshino, Mikio; Itokazu, Yutaka; Nabeshima, Yo Ichi

doi:10.2174/157015905774322507

Cited by 40 publications

(28 citation statements)

References 77 publications

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“…However, even high-tech microsurgical manipulations fail to offer full functional restoration of damaged nerves, creating an unmet need to explore new avenues in the treatment of nerve injury. These include transplantation of Schwann cells, mesenchymal adult precursor cells [2], olfactory ensheathing cells [3] and adipose-derived stem cells [4], and several others are also extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Human adipose-derived stem cells stimulate neuroregeneration

et al. 2015

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Traumatic brain injuries and degenerative neurological disorders such as Alzheimer's dementia, Parkinson's disease, amyotrophic lateral sclerosis and many others are characterized by loss of brain cells and supporting structures. Restoring microanatomy and function using stem cells is a promising therapeutic approach. Among the many various sources, adipose-derived stem cells (ADSCs) are one of the most easily harvested alternatives, they multiply rapidly, and they demonstrate low immunogenicity with an ability to differentiate into several cell types. The objective of this study was to evaluate the effect of xenotransplanted human ADSCs on post-traumatic regeneration of rat sciatic nerve. Peripheral reconstruction following complete sciatic transection and autonerve grafting was complemented by intra-operative injection of hADSCs into the proximal and distal stumps. The injury caused gliosis and apoptosis of sensory neurons in the lumbar 5 (L5) ganglia in the control rodents; however, animals treated with hADSCs demonstrated a smaller amount of cellular loss. Formation of amputation neuroma, which hinders axonal repair, was less prominent in the experimental group, and immunohistochemical analysis of myelin basic protein showed good myelination 65 days after surgery. At this point, control groups still exhibited high levels of microglia/macrophage-specific marker Iba-1 and proliferating cell nuclear antigen, the mark of an ongoing inflammation and incomplete axonal growth 2 months after the injury. This report demonstrates that hADSCs promote neuronal survival in the spinal ganglion, fuel axonal repair and stimulate the regeneration of peripheral nerves.

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Section: Introductionmentioning

confidence: 99%

Human adipose-derived stem cells stimulate neuroregeneration

et al. 2015

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“…These post-natal populations have mesenchymal stem cell (MSC)-like qualities, namely the capacity for self-renewal, the potential to differentiate into multiple lineages, including osteoblasts and chondroblasts, and a potential for in vitro differentiation into cell types from various embryonic layers, including adipose, bone, endothelial and neural-like tissues (Arthur et al, 2008;Cheng et al, 2008;Cordeiro et al, 2008;Fujii et al, 2008;Gay et al, 2007;Harada et al, 1999;He et al, 2009;Honda et al, 2008;Huo et al, 2010). Many researchers have proposed that DPMSCs are promising candidates for the repair and regeneration of a variety of mesenchymal tissues, such as bone, cartilage and muscle (Dezawa et al, 2005;Noël et al, 2002). These findings, together with those of other studies, also suggest that cells from the dental pulp may represent a unique population based on their regenerative potential (About et al, 2000;Gronthos et al, 2000;Iohara et al, 2004;Mina and Braut, 2004;Zhang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Atari

Gil-Recio

Fabregat

et al. 2012

Journal of Cell Science

109

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SummaryDental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. , and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.

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“…1,8,9,10 Thus, the search for new sources of cells with myogenic potential is a relevant problem. 11 Apart from SCs, multipotent mesenchymal stromal cells (MMSC) are considered as a tool for muscle tissue regeneration. MMSC can be differentiated into three orthodox directions: osteo-chondro-and adipogenic, and, under specific conditions, into endothelial and neuronal directions.…”

Section: Introductionmentioning

confidence: 99%

Myogenic potential of human alveolar mucosa derived cells

et al. 2017

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Difficulties related to the obtainment of stem/progenitor cells from skeletal muscle tissue make the search for new sources of myogenic cells highly relevant. Alveolar mucosa might be considered as a perspective candidate due to availability and high proliferative capacity of its cells. Human alveolar mucosa cells (AMC) were obtained from gingival biopsy samples collected from 10 healthy donors and cultured up to 10 passages. AMC matched the generally accepted multipotent mesenchymal stromal cells criteria and possess population doubling time, caryotype and immunophenotype stability during long-term cultivation. The single myogenic induction of primary cell cultures resulted in differentiation of AMC into multinucleated myotubes. The myogenic differentiation was associated with expression of skeletal muscle markers: skeletal myosin, skeletal actin, myogenin and MyoD1. Efficiency of myogenic differentiation in AMC cultures was similar to that in skeletal muscle cells. Furthermore, some of differentiated myotubes exhibited contractions in vitro. Our data confirms the sufficiently high myogenic potential and proliferative capacity of AMC and their ability to maintain in vitro proliferation-competent myogenic precursor cells regardless of the passage number.

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Potential of Bone Marrow Stromal Cells in Applications for Neuro-Degenerative, Neuro-Traumatic and Muscle Degenerative Diseases

Cited by 40 publications

References 77 publications

Human adipose-derived stem cells stimulate neuroregeneration

Human adipose-derived stem cells stimulate neuroregeneration

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Myogenic potential of human alveolar mucosa derived cells

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