Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili

Abstract

Abstract. Nurbailis, Djamaan A, Rahma H, Liswarni Y. 2019. Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili. Biodiversitas 20: 2915-2920. Trichoderma spp. have the potential to be used for controlling the airborne pathogenic fungi such as C. gloeosporioides. The purpose of this study was to evaluate the antifungal activity of the culture filtrate of five isolates of Trichoderma spp. (T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3) against C. gloeosporoides causing anthracnose disease in chili. Culture filtrate of Trichoderma spp. was produced from single culture and dual culture techniques. The design was a Completely Randomized Design with six treatments and four replications. The treatments were culture filtrate from T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3, and negative control (without culture filtrate from Trichoderma spp.). Parameters observed were: the diameter of the colony, colony coverage, conidial germination, and conidial density. The results of this research showed all the culture filtrate from Trichoderma spp. produced by single and dual culture techniques can inhibit the growth of C. gloeosporioides. The culture filtrate from Trichoderma PP2 and T. koningii were the most potential in inhibiting the growth, conidial density, and conidial germination of C. gloeosporioides.

Use of Trichoderma culture filtrates as a sustainable approach to mitigate early blight disease of tomato and their influence on plant biomarkers and antioxidants production

“…In another study, the CFs of various Trichoderma species, including T. harzianum (29.88 mm ) and T. viride (27.13 mm ), demonstrated remarkable suppression of R. oryzae ( Alka and Prajapati, 2017 ). Additionally, the CFs of five Trichoderma species ( T. viride, Trichoderma PP3, T. harzianum , Trichoderma PP2, and T. koningii ) as biofungicides exhibited strong hindrance to C. gloesporioides growth ( Nurbailis et al., 2019 ). Various studies have reported the significant mycelial growth inhibition of various fungal pathogens ( Filizola et al., 2019 ; Mulatu et al., 2022 ), which sturdily confers the biocontrol potential of Trichoderma CFs, and these evidently support the results of the present study.…”

“…represent a significant in vitro and in vivo reduction of a fungal pathogen, Rhizopus oryzae , causing Rhizopus rot of tomatoes ( Alka and Prajapati, 2017 ), when culture filtrates of Trichoderma species were applied. Additionally, the culture filtrates of various Trichoderma species as biofungicides against various fungal pathogens, viz., Colletotrichum gloeosporioides ( Nurbailis et al., 2019 ), against anthracnose of great millet ( Manzar et al., 2021 ), Pythium species, and Phytophthora species, demonstrated inordinate antifungal potential ( Ben M'henni et al., 2022 ). The use of various Trichoderma species has widely been reported as an eco-friendly and safe approach to the control of plant diseases ( Alka and Prajapati, 2017 ; Nurbailis et al., 2019 ; Manzar et al., 2022 ).…”

“…Additionally, the culture filtrates of various Trichoderma species as biofungicides against various fungal pathogens, viz., Colletotrichum gloeosporioides ( Nurbailis et al., 2019 ), against anthracnose of great millet ( Manzar et al., 2021 ), Pythium species, and Phytophthora species, demonstrated inordinate antifungal potential ( Ben M'henni et al., 2022 ). The use of various Trichoderma species has widely been reported as an eco-friendly and safe approach to the control of plant diseases ( Alka and Prajapati, 2017 ; Nurbailis et al., 2019 ; Manzar et al., 2022 ). The application of culture filtrates (CFs) of Trichoderma species as biocontrol not only acts as biostimulants against the inhibition of pathogens but also induces systemic or localized resistance in plants to biotic stresses and, ultimately, as a growth regulator, increases plant biomass ( Abbas et al., 2019 ; Guzmán-Guzmán et al., 2019 ).…”

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“…The results of a study by Alka et al represent a significant in vitro and in vivo reduction of a fungal pathogen, Rhizopus oryzae, causing Rhizopus rot of tomatoes (Alka and Prajapati, 2017), when culture filtrates of Trichoderma species were applied. Additionally, the culture filtrates of various Trichoderma species as biofungicides against various fungal pathogens, viz., Colletotrichum gloeosporioides (Nurbailis et al, 2019), against anthracnose of great millet (Manzar et al, 2021), Pythium species, and Phytophthora species, demonstrated inordinate antifungal potential (Ben M'henni et al, 2022). The use of various Trichoderma species has widely been reported as an eco-friendly and safe approach to the control of plant diseases (Alka and Prajapati, 2017;Nurbailis et al, 2019;Manzar et al, 2022).…”

“…Additionally, the culture filtrates of various Trichoderma species as biofungicides against various fungal pathogens, viz., Colletotrichum gloeosporioides (Nurbailis et al, 2019), against anthracnose of great millet (Manzar et al, 2021), Pythium species, and Phytophthora species, demonstrated inordinate antifungal potential (Ben M'henni et al, 2022). The use of various Trichoderma species has widely been reported as an eco-friendly and safe approach to the control of plant diseases (Alka and Prajapati, 2017;Nurbailis et al, 2019;Manzar et al, 2022). The application of culture filtrates (CFs) of Trichoderma species as biocontrol not only acts as biostimulants against the inhibition of pathogens but also induces systemic or localized resistance in plants to biotic stresses and, ultimately, as a growth regulator, increases plant biomass (Abbas et al, 2019;Guzmań-Guzmań et al, 2019).…”