Potential of DNA markers in detecting divergence and in analysing heterosis in Indian elite chickpea cultivars

Sant, V. J.; Patankar, Aparna G.; Sarode, N. D.; Mhase, L. B.; Sainani, Mohini N.; Deshmukh, R. B.; Ranjekar, P. K.; Gupta, Vidya S.

doi:10.1007/s001220051187

Cited by 86 publications

(62 citation statements)

References 21 publications

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“…While the number of markers and the variation of the reference populations assayed were the strongest factors affecting GD, MD could be affected by environment, genotype x environment interaction, non-additive genetic effects, and the selection criteria used for the development of the cultivars. In agreement with previous reports a poor correlation between the two types of distances was found (Dillmann et al, 1997;Sant et al, 1999).…”

Section: $Vvrfldwlrq Ehwzhhq Glvwdqfh Phdvxuhvsupporting

confidence: 91%

“…Findings on good correlations between the genetic distance of parents (from performance and molecular marker data) and the performance of their hybrids were similar to those reported in cacao and several species (Ghaderi et al, 1984;Shamsuddin, 1985;Cox and Murphy, 1990;Cruz et al, 1994;Ali et al, 1995;Diers et al, 1996;Xiao et al, 1996;Dias and Kageyama, 1997a;Kisha et al, 1997;Lanza et al, 1997;ManjarrezSandoval et al, 1997;Saghai Maroof et al, 1997;Burkhamer et al, 1998). However, there are several reports describing poor correlations (Vaillancourt et al, 1995;Cerna et al, 1997;Helms et al, 1997;Chowdari et al, 1998;Fabrizius et al, 1998;Perenzin et al, 1998;Tenkouano et al, 1998;Bohn et al, 1999;Joyce et al, 1999;Kidwell et al, 1999;Sant et al, 1999). Such contrasting results showed that association between parental divergence and heterosis remained an unsolved question.…”

Section: $Vvrfldwlrq Ehwzhhq Glvwdqfh Phdvxuhvsupporting

confidence: 68%

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Genetic distance and its association with heterosis in cacao

Dias

Marita

Cruz

et al. 2003

Braz. arch. biol. technol.

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The efficiency of cacao breeding program can be increased by choosing superior crosses to be made between divergent clones. We assessed the genetic distance among five clones with RAPD data (genetic distance - GD) and with yield component data (Mahalanobis distance - MD). The clones were evaluated in a diallel, during five years, for five yield components. A total of 130 RAPD bands were scored. GD and MD were used to determine the correlation between genetic distances among clones and the performance of their hybrids. The correlation between GD and MD was 0.67 (P=0.03). Both distances were related to heterotic performance of hybrids for wet seed weight/plant and wet seed weight/fruit. The average hybrid performance for the same two yield components was correlated with only MD. Hence, genetic distances measured by RAPD and yield components can be used as a guide to the choice of the superior crosses.
A eficiência do programa de melhoramento de cacau incrementará pela seleção de cruzamentos superiores entre clones divergentes. Foram estimadas as distâncias genéticas (GD), com dados de RAPD, e de Mahalanobis (MD), com dados de componentes de produção, entre cinco clones. Os clones foram avaliados em um dialelo, por cinco componentes de produção, durante cinco anos. Um total de 130 bandas RAPD foram avaliadas. GD e MD foram utilizadas para cálculo da correlação entre distâncias entre clones e a performance de seus híbridos. A correlação entre GD e MD foi de 0.67 (P=0.03). Ambas as distâncias foram correlacionadas à performance heterótica dos híbridos para peso de sementes frescas/planta e peso de sementes frescas/fruto. A performance híbrida média para os mesmos componentes de produção foi correlacionada somente com MD. Assim, distâncias genéticas medidas por RAPD e componentes de produção podem ser usadas como um guia para a escolha de cruzamentos superiores

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Section: $Vvrfldwlrq Ehwzhhq Glvwdqfh Phdvxuhvsupporting

confidence: 91%

Section: $Vvrfldwlrq Ehwzhhq Glvwdqfh Phdvxuhvsupporting

confidence: 68%

Genetic distance and its association with heterosis in cacao

Dias

Marita

Cruz

et al. 2003

Braz. arch. biol. technol.

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“…The genetic distance values ranged from 0.09 to 0.27 based on RAPD analysis, whereas genetic distance ranged from 0.42 to 0.61 with oligonucleotide probes. The experimental findings in our laboratory also supported the findings of Sant et al (1999). Out of 78 RAPD primers tested, only 20 primers revealed polymorphisms between the chickpea cultivars (Singh et al 2003).…”

Section: Rflp and Rapd Markerssupporting

confidence: 85%

“…The low polymorphism may have been caused by narrow ancestry and self-pollinated nature of the crop (Van Rheenen, 1992). Sant et al (1999) employed RAPD and oligonucleotide probes to assess genetic diversity between 29 elite Indian chickpea cultivars. Out of 35 RAPD primers tested, only 10 primers (29%) generated polymorphic patterns between the cultivars, which indicate narrow genetic base of the cultivated species.…”

Section: Rflp and Rapd Markersmentioning

confidence: 99%

Chickpea Improvement: Role of Wild Species and Genetic Markers

Singh

Sharma

Varshney

et al. 2008

Biotechnology and Genetic Engineering Reviews

111

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Chickpea is an important grain legume of the semi arid tropics and warm temperate zones, and forms one of the major components of human diet. However, a narrow genetic base of cultivated chickpea (Cicer arietinum L.) has hindered the progress in realizing high yield gains in breeding programs. Furthermore, various abiotic and biotic stresses are the major bottlenecks for increasing chickpea productivity. Systematic collection and evaluation of wild species for useful traits has revealed presence of a diverse gene pool for tolerance to the biotic and abiotic stresses. Relationships among the species of genus Cicer are presented based on crossability, karyotype and molecular markers. The reproductive barriers encountered during interspecific hybridization are also examined. Recent information on genetic linkage maps, comparison of isozymes

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“…Previous studies have shown that RAPD fingerprinting of cultivated chickpea was unable to reveal DNA polymorphism. Low level of polymorphism with RAPD has been reported by Sonnante et al (1997) and Sant et al (1999). These workers studied the potential of DNA markers in detecting divergence in chickpea and reported the inability of RAPD markers to reveal a high level of DNA polymorphism.…”

Section: Discussionmentioning

confidence: 90%

Use of RAPD Markers in Comparison with Agro-morphological Traits for Estimation of Diversity among Chickpea Genotypes

Bakhsh¹,

Iqbal²,

Rahman³

et al. 2017

IJAB

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To cite this paper: Bakhsh, A., S.M. Iqbal, M.U. Rahman and A. Javaid, 2017. Use of RAPD markers in comparison with agro-morphological traits for estimation of diversity among chickpea genotypes. AbstractGenetic diversity was assessed among 38 chickpea (Cicer arietinum L.) genotypes on the basis of random amplified polymorphic DNA (RAPD) in comparison with agro-morphological traits. Evaluation of agro-morphological traits revealed highly significant differences among genotypes. Days to 50% flowering ranged from 92-118, plant height 54.16-87 cm, number of fruit bearing branches 4-17.25, number of pods per plant 7.6-27.4, and grain yield per plant 3.5-9.8 g. Ascochyta blight (caused by Ascochyta rabiei) score of these genotypes was recorded on 1-9 rating scale that varied from 3-9. Cluster analysis showing relationship based on morphological traits (scale: Euclidean distance) placed 35 genotypes into five distinct groups, while three genotypes namely Noor-91, Local Mankera and BR4 did not include in any cluster. RAPD analysis showed that 35 RAPD primers amplified a total of 212 fragments out of which 45 were polymorphic. Polymorphic bands were generated by 21 primers whereas 14 primers were monomorphic. Genetic similarity matrix based on Nei and Li's index revealed similarity coefficients ranging from 92-97% indicating lower level of genetic polymorphism revealed by RAPD primers. Dandrogram constructed on the basis of these coefficients grouped all the genotypes into 2 major and 3 small clusters at 92% similarity level. Two decamers, OPC5 and OPC14 distinguished between three Desi and two Kabuli genotypes. This study showed that the level of genetic variability observed in chickpea for agro-morphological traits was not reflected in DNA polymorphism obtained by RAPD analysis.

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Potential of DNA markers in detecting divergence and in analysing heterosis in Indian elite chickpea cultivars

Cited by 86 publications

References 21 publications

Genetic distance and its association with heterosis in cacao

Genetic distance and its association with heterosis in cacao

Chickpea Improvement: Role of Wild Species and Genetic Markers

Use of RAPD Markers in Comparison with Agro-morphological Traits for Estimation of Diversity among Chickpea Genotypes

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