Potential of Enterococcus faecium LM5.2 for lipopeptide biosurfactant production and its effect on the growth of maize (Zea mays L.)

Abstract

The lipopeptide biosurfactants' chemical characteristics from the lactic acid bacteria isolated from milk and milk products were studied and their effect on maize plant growth. The oil displacement test was performed as a primary screening method to select the BS producing bacteria. Enterococcus faecium LM5.2 had the maximum emulsi cation index of 45.1±3 and reduced the surface tension to 32.98 ± 0.23% among all the isolates. E. faecium LM5.2 e ciently produced 945.26 ± 4.62 mg/l biosurfactants within 48 hours in MRS broth under the optimum conditions. The con rmation of the identity of the isolate LM5.2 was done with physiochemical tests and 16S rRNA gene sequencing. The molecular phylogenetic relationship was evaluated by the Neighbour-Joining phylogenetic method. The biosurfactant was puri ed by TLC and identi ed as lipopeptide-like iturines and surfactins based on R f values. Mass spectroscopy, NMR, and FTIR analysis also con rmed the biosurfactant's identity as the derivatives of iturin and surfactin. Both the biosurfactant and its producer bacterium were evaluated for their plant growth-promoting activity, and it was found that the biosurfactant and the bacterium could enhance plant growth. To the best of our knowledge, this is the rst report of lipopeptide biosurfactant production from Enterococcus faecium. Moreover, the study also showed that the biosurfactant and biosurfactant producing E. faecium LM5.2 could be an eco-friendly plant growth-promoting agent.

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

“…Furthermore, this study revealed the growth-promoting properties of E. italicus ONU547 for microclones during the transition from in vitro conditions to a nonsterile environment. Moreover, our experiments demonstrated the positive effects of inoculating with cell suspensions of the microorganisms rather than their purified metabolites, as often reported in the existing literature (Lee et al, 2015 ; Pellegrini et al, 2020 ; Chaurasia et al, 2022 ; Msimbira et al, 2022 ). This indicates that for the potential development of biological preparations based on E. italicus ONU547, there is an opportunity to save time by bypassing the isolation and purification of specific synthesized compounds and simply utilizing the cell mass.…”

“…In other studies investigating the effects of enterococci on plant growth, different concentrations of bacteria, inoculation procedures, and plant species were employed, resulting in varying observed influences. For example, Chaurasia et al ( 2022 ) inoculated soil with Enterococcus spp. at a concentration of 8 × 10 5 CFU/kg, leading to maize seed germination occurring three days faster than in the control group and a 53.7% increase in plant height, while no significant difference in the number of leaves was observed.…”

Effect of Glycolipoprotein Biosurfactant from Enterococcus faecium on the viability of Breast cancer cell lines.

“…According to Anschau standard curve the lipid content was 33% and the concentration of the protein was 28.5%, according to an analysis of the protein's absorbance using the Bradford standard curve. These results compared with Chaurasia et al, 34 who reported that biosurfactant produced by E. faecium LM5 consisted of Lipid and peptides.…”

“…While Lara et al, 34 mentioned that the biosurfactant produced by Lactiplantibacillus plantarum Tw226 consisted of protein as the main part (64%), then Lipids (21%), and carbohydrate (21%).…”