Potential of Escherichia coli O157:H7 to persist and form viable but non-culturable cells on a food-contact surface subjected to cycles of soiling and chemical treatment

Marouani-Gadri, Nesrine; Firmesse, Olivier; Chassaing, Danielle; Nielsen, Dennis Sandris; Arneborg, Nils; Carpentier, Brigitte

doi:10.1016/j.ijfoodmicro.2010.09.002

Cited by 39 publications

(24 citation statements)

References 42 publications

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“…Although sanitizer treatments were effective at reducing significantly the viability and the metabolism of STEC in biofilms, the biofilm matrix either remained or was partly removed depending on the sanitizer (Table 2). These results agree with previous reports (6,7,68,69). Moreover, no significant differences in viability or metabolic activity were seen between seropathotypes or isolates (7).…”

Section: Discussionsupporting

confidence: 93%

“…Standard cleaning and sanitizing practices are used to reduce sources of microbial contamination on their products. As previously described, strong attachment of biofilms on surfaces may affect the efficiency of sanitizers and protect bacteria against sanitation protocols that are used to reduce contamination (7,68,69). In the present study, we examined if sanitizers commonly used in good sanitation practices were effective at both reducing STEC viability in biofilms and eliminating the biofilm matrix.…”

Section: Discussionmentioning

confidence: 83%

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Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

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bForming biofilms may be a survival strategy of Shiga toxin-producing Escherichia coli to enable it to persist in the environment and the food industry. Here, we evaluate and characterize the biofilm-forming ability of 39 isolates of Shiga toxin-producing Escherichia coli isolates recovered from human infection and belonging to seropathotypes A, B, or C. The presence and/or production of biofilm factors such as curli, cellulose, autotransporter, and fimbriae were investigated. The polymeric matrix of these biofilms was analyzed by confocal microscopy and by enzymatic digestion. Cell viability and matrix integrity were examined after sanitizer treatments. Isolates of the seropathotype A (O157:H7 and O157:NM), which have the highest relative incidence of human infection, had a greater ability to form biofilms than isolates of seropathotype B or C. Seropathotype A isolates were unique in their ability to produce cellulose and poly-N-acetylglucosamine. The integrity of the biofilms was dependent on proteins. Two autotransporter genes, ehaB and espP, and two fimbrial genes, z1538 and lpf2, were identified as potential genetic determinants for biofilm formation. Interestingly, the ability of several isolates from seropathotype A to form biofilms was associated with their ability to agglutinate yeast in a mannose-independent manner. We consider this an unidentified biofilmassociated factor produced by those isolates. Treatment with sanitizers reduced the viability of Shiga toxin-producing Escherichia coli but did not completely remove the biofilm matrix. Overall, our data indicate that biofilm formation could contribute to the persistence of Shiga toxin-producing Escherichia coli and specifically seropathotype A isolates in the environment. Shiga toxin-producing Escherichia coli (STEC) strains, including enterohemorrhagic E. coli (EHEC), are food-borne and waterborne human enteric pathogens responsible for infections. They are associated with important public health concerns in developed countries. Symptoms associated with STEC infections range from abdominal cramps and bloody diarrhea to postinfectious complications, such as hemolytic-uremic syndrome (HUS). HUS, a life-threatening complication of STEC infections, is a consequence of Shiga toxin production. According to the Public Health Agency of Canada (PHAC), more than 30,000 cases of STEC infections occur each year in Canada (1). Environmentally, the main reservoir for STEC is cattle. Transmission of STEC to humans typically is associated with the consumption of contaminated food such as undercooked beef, fresh produce, unpasteurized milk, or contaminated drinking water (2), but these pathogens also can be transmitted from person to person or via direct contact with animals or their feces.The 2003 Karmali seropathotype model classifies STEC into seropathotypes based on their reported incidence in human disease, outbreaks, and/or association with the development of severe symptoms in humans (3). Serotypes frequently responsible for hemorrhagic colitis (HC) and HUS, ...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 83%

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

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show abstract

“…The approach is made possible by EMA's efficiency to penetrate dead cells even at relatively low dye concentrations. Whereas early studies employing EMA typically used final dye concentrations of 100 μg/ml (238 μM) (Douglas Inglis et al, 2010;Flekna et al, 2007;Graiver et al, 2010;Marouani-Gradi et al, 2010;Rudi et al, 2005a), concentrations in more recent studies were chosen substantially lower. Cawthorn and Witthuhn (2008) have found that the use of three different EMA concentrations with viable Enterobacter sakazakii cells − 100 (238 μM), 50 (119 μM), and 10 μg/ml (23.8 μM) -resulted in very different PCR product yields of 74, 82 and 92%, respectively (compared with 95, 96, and 97% after PMA treatment).…”

Section: Emamentioning

confidence: 99%

Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification

Fittipaldi

Nocker

Codony

2012

Journal of Microbiological Methods

350

283

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“…Considerable research has been directed at evaluating the impact of STEC biofilms on food safety, as well as understanding the mechanisms and genetic basis for biofilm formation by these pathogens (3,11,25,27,38,39). Studies also have investigated the tolerance of STEC cells in biofilms to decontamination reagents (18,19,26,40).…”

mentioning

confidence: 99%

Biofilm Formation by Shiga Toxin–Producing Escherichia coli O157:H7 and Non-O157 Strains and Their Tolerance to Sanitizers Commonly Used in the Food Processing Environment

Wang

Bono

Kalchayanand

et al. 2012

Journal of Food Protection

113

101

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Shiga toxin-producing Escherichia coli (STEC) strains are important foodborne pathogens. Among these, E. coli O157:H7 is the most frequently isolated STEC serotype responsible for foodborne diseases. However, the non-O157 serotypes have been associated with serious outbreaks and sporadic diseases as well. It has been shown that various STEC serotypes are capable of forming biofilms on different food or food contact surfaces that, when detached, may lead to cross-contamination. Bacterial cells at biofilm stage also are more tolerant to sanitizers compared with their planktonic counterparts, which makes STEC biofilms a serious food safety concern. In the present study, we evaluated the potency of biofilm formation by a variety of STEC strains from serotypes O157:H7, O26:H11, and O111:H8; we also compared biofilm tolerance with two types of common sanitizers, a quaternary ammonium chloride-based sanitizer and chlorine. Our results demonstrated that biofilm formation by various STEC serotypes on a polystyrene surface was highly strain-dependent, whereas the two non-O157 serotypes showed a higher potency of pellicle formation at air-liquid interfaces on a glass surface compared with serotype O157:H7. Significant reductions of viable biofilm cells were achieved with sanitizer treatments. STEC biofilm tolerance to sanitization was strain-dependent regardless of the serotypes. Curli expression appeared to play a critical role in STEC biofilm formation and tolerance to sanitizers. Our data indicated that multiple factors, including bacterial serotype and strain, surface materials, and other environmental conditions, could significantly affect STEC biofilm formation. The high potential for biofilm formation by various STEC serotypes, especially the strong potency of pellicle formation by the curli-positive non-O157 strains with high sanitization tolerance, might contribute to bacterial colonization on food contact surfaces, which may result in downstream product contamination.

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Potential of Escherichia coli O157:H7 to persist and form viable but non-culturable cells on a food-contact surface subjected to cycles of soiling and chemical treatment

Cited by 39 publications

References 42 publications

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification

Biofilm Formation by Shiga Toxin–Producing Escherichia coli O157:H7 and Non-O157 Strains and Their Tolerance to Sanitizers Commonly Used in the Food Processing Environment

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