Potential of essential oils for protection of Couscous against Aspergillus flavus and aflatoxin B1 contamination

Miri, Yamina Ben; Benabdallah, Amina; Taoudiat, Aldjia; Mahdid, Mohamed; Djenane, Djamel; Tacer-Caba, Zeynep; Topkaya, Cansu; Simal-Gándara, Jesús

doi:10.1016/j.foodcont.2022.109474

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…The treatments applied showed a greater than 50% protection of cereal grains from A. ochraceus and A. niger contamination. These results are in line with other studies in wheat grains [10] and couscous [30], where percent protection against A. flavus ranged from 52 to 77% and from 50 to 86%, respectively. For A. ochraceus, the highest protective effect of menthol and eugenol was found in rice, where fungal counts in the control group (41 × 10 3 cfu/g) dropped by 63.4% to 15 × 10 3 cfu/g and by 70.7% to 12 × 10 3 cfu/g, respectively.…”

Section: Effects Of Natural Antifungals On Mycelial Growthsupporting

confidence: 92%

Antifungal Activity of Menthol, Eugenol and Their Combination against Aspergillus ochraceus and Aspergillus niger In Vitro and in Stored Cereals

Miri

Nouasri

Herrera

et al. 2023

Foods

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Aspergillus ochraceus and Aspergillus niger are spoilage and mycotoxin-producing fungi that can contaminate agricultural commodities and derived products. In the present study, menthol, eugenol, and their combination (mix 1:1) were tested to determine their contact and fumigation toxicity against the two fungi. Menthol, eugenol, and their mixture significantly reduced mycelial growth and spore germination at concentrations from 300 to 600 µg/mL, and the inhibitory effects showed clear dose dependence. The minimum inhibitory concentration (MIC) values against A. ochraceus were 500 µg/mL (menthol), 400 µg/mL (eugenol), and 300 µg/mL (mix 1:1), while the MIC values for A. niger were 500 µg/mL (menthol), 600 µg/mL (eugenol), and 400 µg/mL (mix 1:1). Additionally, the analyzed compounds exhibited more than 50% protection against A. ochraceus and A. niger by fumigation of stored cereal grains (maize, barley, and rice) in sealed containers. The binary mixture of menthol and eugenol showed synergistic effects against both fungi in both in vitro direct contact and stored grain fumigation trials. The results of the present study provide a scientific basis for the application of a combination of natural antifungals in food preservation.

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Section: Effects Of Natural Antifungals On Mycelial Growthsupporting

confidence: 92%

Antifungal Activity of Menthol, Eugenol and Their Combination against Aspergillus ochraceus and Aspergillus niger In Vitro and in Stored Cereals

Miri

Nouasri

Herrera

et al. 2023

Foods

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“…Major components of EOs vis. Thymol, eugenol, anethole, menthol, citral, pinenes, cinnamaldhydes, carvacrol, and carvone have been extensively studied concerning their antimicrobial spectrum against different microorganisms, including bacteria and fungi [ 19 , 20 ]. Thus, eugenol is a phenolic component that is mostly taken from buds and leaves of clove and from cinnamon.…”

Section: Resultsmentioning

confidence: 99%

Antifungal effects of selected menthol and eugenol in vapors on green coffee beans during long-term storage

Miri¹,

Nouasri²,

Benabdallah³

et al. 2023

Heliyon

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“…Aflatoxin B 1 (AFB 1 ) is a secondary metabolite produced by Aspergillus flavus and A. parasiticus 1,2 and has been linked to mutagenicity, 3 teratogenicity, 4 carcinogenicity 5 and immunotoxicity 6 . Large quantities of grains, especially corn, are contaminated with AFB 1 annually 7‐9 .…”

Section: Introductionmentioning

confidence: 99%

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

Lou,

Li,

Yang

et al. 2023

J Sci Food Agric

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BackgroundCorn, being an important grain, is prone to contamination by aflatoxin B1 (AFB1), and AFB1‐contaminated corn severely endangers the health of humans and livestock. Trametes versicolor, a fungus that can grow in corn, possesses the ability to directly degrade AFB1 through its laccase. This study aimed to optimize the fermentation conditions for T. versicolor to degrade AFB1 in corn and investigate the effect of T. versicolor fermentation on the nutritional composition of corn. AFB1‐contaminated corn was used as the culture substrate for T. versicolor. A combination of single factor experiments and response surface methodology was employed to identify the optimal conditions of AFB1 degradation.ResultsThe results showed that the optimal conditions of AFB1 degradation were as follows: 9 days of fermentation, a fermentation temperature of 26.7 °C, a moisture content of 70.5%, and an inoculation amount of 4.9 mL (containing 51.99 mg of T. versicolor mycelia). With the optimal conditions, the degradation rate of AFB1 in corn could reach 93.01%, and the dry basis content of protein and dietary fiber in the fermented corn was significantly increased. More importantly, the lysine content in the fermented corn was also significantly increased.ConclusionThis is the first report that direct fermentation of AFB1‐contaminated corn by T. versicolor not only efficiently degrades AFB1 but also improves the nutritional composition of corn. These findings suggest that the fermentation of corn by T. versicolor is a promising, environmentally friendly, and efficient approach to degrade AFB1 and improve the nutritional value of corn.This article is protected by copyright. All rights reserved.

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Potential of essential oils for protection of Couscous against Aspergillus flavus and aflatoxin B1 contamination

Cited by 10 publications

References 42 publications

Antifungal Activity of Menthol, Eugenol and Their Combination against Aspergillus ochraceus and Aspergillus niger In Vitro and in Stored Cereals

Antifungal Activity of Menthol, Eugenol and Their Combination against Aspergillus ochraceus and Aspergillus niger In Vitro and in Stored Cereals

Antifungal effects of selected menthol and eugenol in vapors on green coffee beans during long-term storage

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

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Potential of essential oils for protection of Couscous against Aspergillus flavus and aflatoxin B1 contamination

Cited by 10 publications

References 42 publications

Antifungal Activity of Menthol, Eugenol and Their Combination against Aspergillus ochraceus and Aspergillus niger In Vitro and in Stored Cereals

Antifungal Activity of Menthol, Eugenol and Their Combination against Aspergillus ochraceus and Aspergillus niger In Vitro and in Stored Cereals

Antifungal effects of selected menthol and eugenol in vapors on green coffee beans during long-term storage

Optimizing the degradation of aflatoxin B1 in corn by Trametes versicolor and improving the nutritional composition of corn

Contact Info

Product

Resources

About

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn