Background: The successful growth and development of the vanilla plant are supported by the availability of soil nutrients that are absorbed by the plant roots. Natural fertilizers derived from plant waste easily decompose in the soil so that they are more quickly absorbed by roots and stored in plant cells. The purpose of this study was to determine the effect of eco-enzymes and husk charcoal on the growth of vanilla seedlings. Methods: This study was designed using a complete randomized block design. The first factor was eco-enzyme (without eco-enzyme and with eco-enzyme 15 ml/plant), namely E0 and E1, the second factor was the husk charcoal dose (0, 5, 10, 15) g/plant, P0, P1, P2, P3. Data were analyzed using ANOVA analysis of variance with Duncan’s multiple range test (DMRT) with a significant difference of 5%. The results showed that the best vanilla growth was obtained by treating it with 15 ml/polybag eco-enzyme and 15 g/polybag coconut shell charcoal (E1P3). Result: The plant height reaches 79.67 cm, the number of leaves reaches 12.67 strands and the width reaches 405.42 mm. The fresh weight of the plants reached 71.33 g, the dry weight of the plants reached 28 g, the fresh weight of the roots reached 8.6 g, and the dry weight of the roots reached 1.27 g. Microscopi observation showed that eco enzymes and coconut shell charcoal infected the roots and filled the tissues, then played a role in increasing the growth of the vanilla seedlings.