Potential of fungus Trichoderma harzianum for toxicity reduction in municipal solid waste leachate

Abstract

The purpose of this research was to test the ability of selected fungus Trichoderma harzianum for municipal solid waste leachate treatment. The research carried out by inoculating the enzyme produced by T. harzianum with different ratio (10% up to 80% v/v) of solid waste leachate. The findings clearly indicated that the enzymatic addition was effectively acted (86.09% of chemical oxygen demand removed), and the rate of chemical degradation amended treatment enhanced as compared to control. Also, the enzymatic addition excellently reduced the inhibition of germination (35.8 ± 0.7) of seed (Zea mays L.) and improved the environmental quality of treated leachate. Therefore, these results can be use to articulate the preliminary feedstock for pilot to fieldscale application.

Leachates and natural organic matter. A review of their biotreatment using fungi

“…Cucumis sativus (Tigini et al, 2014), Sinapis alba (Kalcikova et al, 2014) or Zea mays (Awasthi et al, 2017) seeds, the inhibition of algal growth using Pseudokirchneriella subcapitata (Tigini et al, 2013) or Raphidocelis subcapitata (Spina et al, 2018), the mobility inhibition of the fresh water crustacean Ceriodaphnia dubia (Tigini et al 2014) or by means of alkaline single-cell gel electrophoresis (Comet assay) (Gosh et al, 2014).…”

“…Among the available studies, that carried out by Tigini et al (2014) Another difference between the studies on leachates and on NOM is the scale of the tests and the state of the microorganisms. As previously reported, all the NOM experiments with fungi were done in flasks, whereas in this section, as well as flasks (Awasthi et al, 2017;Bardi et al, 2017b;Ellouze et al, 2008;Ellouze et al, 2009;Hu et al, 2017;Hu et al, 2016;Kalčíková et al, 2014;Saetang, 2009;Saetang & Babel, 2010a;Saetang & Babel, 2010b;Tigini et al, 2014;Tigini et al, 2013;Wan Razarinah et al, 2014;Wan Razarinah et al, 2015), other reactor configurations were employed, such as fixed bed (Faco and Santaella, 2002;Gao et al, 2004;Saetang, 2009;Saetang and Babel, 2009;Abdullah et al, 2013;Bardi et al 2017a) or membrane bioreactors (Wichitsathian et al, 2006;Brito et al, 2012;Brito, 2013;Amaral et al, 2017ab;Reis et al, 2017). To our knowledge, there are no studies dealing with the biodegradation of leachate by a fungus, comparing the results obtained with different reactor configurations.…”

“…When the initial toxicity of the leachate is too high, the stream is commonly diluted before treatment in order to improve the efficiency of the fungus. The dilutions employed differ between articles, but 50% (v/v) is the most prevalent (Abdullah et al, 2013;Awasthi et al, 2017;Gao et al, 2004;Kalčíková et al, 2014;Saetang & Babel, 2009). More specifically, Ellouze et al ( 2008) evaluated the biodegradation by several fungi of a young leachate at different dilutions: 10, 30, 50, 70 and 100% leachate.…”

“… The authors suggested that the complementary effect of the two cosubstrates could be exploited by adding glucose as start-up and cellulose in a second step of the treatment. They proposed non-continuous cosubstrate addition too, for reducing the costs of continuous cosubstrate dosage Awasthi et al (2017)  Leachate from the municipal solid waste dumping site Katondha, Jabalpur (India)  Crude enzyme filtrate from Trichoderma harzianum (FGCCA29)…”

Screening and selection of autochthonous fungi from leachate contaminated-soil for bioremediation of different types of leachate

“…Fungi can propagate in air over long distances by producing non-motile spores that may or may not be the product of mating between two compatible hyphae [64]. Presumably, the fungi isolated from these three contaminated soils possessed the catabolic potential necessary for the degradation of some organic compounds in landfill leachate [62]. Furthermore, they are generally able to tolerate the high toxicity and extreme physical parameters (i.e., high ammonia concentration), which is commonly found in landfill leachate.…”