Potential of Integrating Model-Based Design of Experiments Approaches and Process Analytical Technologies for Bioprocess Scale-Down

Neubauer, Peter; Anane, Emmanuel; Junne, Stefan; Bournazou, Mariano Nicolás Cruz

doi:10.1007/10_2020_154

Cited by 7 publications

(4 citation statements)

References 110 publications

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“…With lifelines, digital twins of the bioreactor (macro-twin) and the cell (micro-twin) can be established (Hajian et al, 2020). Digital twins can help in the scale-up process by reducing the need for costly pilot runs and facilitating the optimisation procedure or they could be run in parallel to the large-scale fermentation to predict performance in real time (Hajian et al, 2020;Neubauer et al, 2021).…”

Section: Tablementioning

confidence: 99%

Microbial lifelines in bioprocesses: From concept to application

Blöbaum

Haringa

Grünberger

2023

Biotechnology Advances

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Section: Tablementioning

confidence: 99%

Microbial lifelines in bioprocesses: From concept to application

Blöbaum

Haringa

Grünberger

2023

Biotechnology Advances

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“…Mobile robotic lab assistants allow the integration of different laboratory equipment and different cultivation scales in a common automated platform. Thus, large amounts of necessary process data from different scales can be generated in an automated environment for faster scale-up and reduced timelines (1, 35).…”

Section: Introductionmentioning

confidence: 99%

“…Mobile robotic lab assistants allow the inte-gration of different laboratory equipment and different cultivation scales in a common automated platform. Thus, large amounts of necessary process data from different scales can be generated in an automated environment for faster scale-up and reduced timelines (1,35). Automated cultivation platforms, mobile robotic technologies and high-throughput analyzers offer potential for flexible and accelerated upstream process development (31).…”

Section: Introductionmentioning

confidence: 99%

Automated bioprocess feedback operation in a high throughput facility via the integration of a mobile robotic lab assistant

Kaspersetz

Waldburger

Schermeyer

et al. 2022

Preprint

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Biotechnological processes development is challenging due to the sheer variety of process parameters. For efficient upstream development parallel cultivation systems have proven to reduce costs and associated timelines successfully, while offering excellent process control. However, the degree of automation of such small scale systems is comparably low and necessary sample analysis requires manual steps. Although the subsequent analysis can be performed in a high-throughput manner, the integration of analytic devices remains challenging. Especially, when cultivation and analysis laboratories are spatially separated. Mobile robots offer a potential solution, but the implementation in research laboratories is not widely adopted. Our approach demonstrates the integration of a small scale cultivation system into a liquid handling station for an automated sample procedure. The samples are transferred via a mobile robotic lab assistant and subsequently analysed by a high-throughput analyzer. The process data is stored in a centralized database. The mobile robotic workflow guarantees a flexible solution for device integration and facilitates automation. Restrictions regarding spatial separation of devices are circumvented, enabling a modular platform throughout different laboratories. The presented cultivation platform is evaluated based on industrial relevant E. coli BW25113 high cell density fed-batch cultivation. Here its suitability for accelerating bioprocess development is proven. The necessary magnesium addition for reaching high cell densities in mineral salt medium is automated via a feedback operation loop. The feedback operation loop demonstrates the possibility for advanced control options. This study sets the foundation for a fully integrated facility with different cultivation scales sharing the same data infrastructure, where the mobile robotic lab assistant physically connects the devices.

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“…These tools are valuable in scale-up and scale-down studies where the fermentation process is transferred from the laboratory scale to a larger industrial scale or vice versa. This helps to maintain consistent conditions and ensure reproducibility [22].…”

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confidence: 99%