Potential of Leucas zeylanica extract to eliminate E. coli and S. aureus in Corbicula fluminea (“Etak”) tissue

Abstract

“Etak” or Corbicula fluminea, is a freshwater mollusc species regularly consumed as a popular snack among the Kelantanese in Malaysia. The “etak” is usually heated with traditional smoking process which is considered as half cooked and the smoked C. fluminea is commonly known as “etak salai”. This study focuses on the potential of Leucas zeylanica leaves extract to eliminate the bacteria content in “etak salai”. Extraction of bacterial genomic DNA was performed and confirmed the existence of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in “etak salai”. Antibacterial properties of L. zeylanica leaves extract was identified using disc diffusion assay and the result obtained exhibit that 70 μg/μL of L. zeylanica extract was the optimum concentration to give the effect of 11 mm inhibition zone for E. coli and 15 mm inhibition zone for S. aureus. This finding proof that L. zeylanica leaves could be the ingredients in the paste for “etak salai” preparation.

Phytochemicals from Leucas zeylanica Targeting Main Protease of SARS-CoV-2: Chemical Profiles, Molecular Docking, and Molecular Dynamics Simulations

“…The antimalarial drugs chloroquine and hydroxychloroquine have been suggested as potential anti-COVID-19 therapies in recent studies; therefore, the traditional use of L. zeylanica against malaria may be significant [30]. The ethnopharmacological activities of this plant, including anti-inflammatory, antidiarrheal, antimicrobial, antioxidant, thrombolytic, hepatoprotective, analgesic, larvicidal, and insecticidal activities, have been reported in previous studies [25,26,31]. Importantly, the plant exhibits significant antioxidant and thrombolytic properties, which may be useful against these components of the SARS-CoV-2 pathogenesis.…”

Evaluation of The In-Vitro Anthelmintic Activity of Leucas zeylanica Extracts on Earthworms

“…The plants were then air-dried on the bench at room temperature (30 °C) for 7 days until fully dried. The ariel part of the plants was dried until crispy texture (Abdullah et al, 2019).…”

“…Leucas zeylanica or "Ceylon slitwort" locally known as "Ketumbit" (Malay) is a plant native to Tropical Asia and Malaya, abundantly distributed in the bushes of Malaysia, especially Kelantan (Abdullah et al, 2019). This plant is often cultivated in home gardens for use in local medicine and as a pot herb where this popular, multi-purpose medical plant has a variety of medicinal uses for different ailments including treating colds, expelling intestinal worms, ameliorating cough, and promoting digestion.…”

Leucas zeylanica is a Bangladeshi plant with significant medicinal prospect: A review

“…Abdullah et al explored that the methanolic leaf extract (70 μg/μL per well) gave a clear zone of 10.6 and 14.8 mm for E. coli and S. aureus, respectively. Here, Gentamicin formed 15.0 (E. coli) and 16.4 mm (S. aureus) clear zone [48]. Another study pronounced that ethanolic extract (600 μg/disc) of L. zeylanica yielded 10-21 mm inhibition zone against some bacteria [39].…”