Potential of Ni(II)-NTA-Modified Poly(ethylene imine) Glycopolymers as Carrier System for Future Dendritic Cell-Based Immunotherapy

Abstract

Dendritic cells (DCs) play a crucial role in the development of cell-mediated immunotherapy due to their ability to induce and maintain strong immune responses. In our study, we evaluated a biocompatible Ni(II)-NTA-modified poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) as new carrier system to increase the antigen uptake into iDCs for future DC-based immunotherapy. Ni(II)-NTA-DG led to an increase in His6-Gp160 uptake in monocytes and iDCs, where His6-Gp160 is localized in the early endosomal and lysosomal compartments. Ni(II)-NTA-DG and the formed polyplexes induced an activation of iDCs, showing an increasing expression of costimulatory molecules CD86, CD80, and proinflammatory cytokines IL-6 and IL-8. Beside no influencing effect of Ni(II)-NTA-DG and polyplexes on the maturation of antigen-bearing DCs, the mature peptide bearing DCs remained their ability to migrate along a gradient of CCR7 ligands. Thus, Ni(II)-NTA-DG with advancing biological properties is a promising carrier system for the future application in DC-based immunotherapy.

Dendritic glycopolymers based on dendritic polyamine scaffolds: view on their synthetic approaches, characteristics and potential for biomedical applications

“…7 and 8). 10,109 Most of the dendritic glycopolymers used as drug delivery systems and therapeutics and diagnostics for neurodegenerative disease, 10,[54][55][56][57][58][59][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76]110,113,114,[119][120][121][122][123][124] belong to the type of open shell dendritic glycopolymers, while some perfectly branched glycodendrimers, mainly applied as sugarballs in the field of neurodegenerative disease [109][110][111][112][113][114][115][116][117][118] and in drug delivery system, 56,60,61,77 possess a dense shell. Most of the dense shell glycoarchitectures used in biomedical applications are based on PPI dendrimer scaffolds.…”

“…Further attention is now directed to the molecular characteristics of open and dense shell dendritic glycopolymers, based on PPI dendrimer and PEI, 10,55,56,60,61,[76][77][78][79][80][81][82][83][84][108][109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][124] used as a drug delivery system and as sugar balls for therapeutics and diagnostics in neurodegenerative diseases. Playing with the degree of mono-, disaccharide and oligosaccharide functionalization on those dendritic polyamine scaffolds, surface charge or charge density of PPI glycodendrimers is tunable from positive for open shell glycodendrimers to neutral for dense shell glycodendrimers.…”

Improved epoxy thermosets by the use of poly(ethyleneimine) derivatives

“…introduced mono-and oligosaccharide units on the PEI surface by reductive amidation or N-carboxyanhydride polymerization [129,130]. These defined novel oligosaccharide architectures on dendritic polymer surfaces have been studied as nano-sized carrier systems for gene delivery [131,132] or metal nanoparticles [133,134].…”

Influence of core and maltose surface modification of PEIs on their interaction with plasma proteins—Human serum albumin and lysozyme

“…In this context sugar-coated poly(propylene imine) (PPI) dendrimers are characterized by excellent biocompatibility [16][17][18], and at the same time many promising and advantageous biological and delivery properties have been determined that are necessary for their efficient use in biomedicine [19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Dendrimers and hyperbranched polymers belong to the wide group of dendritic architecture polymers.…”