Potential of orchid as antifungal agent resources: a scoping review

Abstract

In 2017, one billion people contracted fungal infection with 11.5 million people contracted life-threatening infection and 1.5 million death per year. Orchid is a plant that grows in tropical and sub-tropical countries, the same places where fungal infection occurrence is high. On previous studies reported that orchid contains alkaloids and polyphenols such as flavonoid, and phenol acid. The review aimed to identify the orchid as a potential antifungal resource. A scoping review was used. The type of articles reviewed were original articles obtained from five electronic journal databases published within 2012-2021. Three articles reported similar result which was that orchid extract has a significant antifungal activity although the part of plant used was different, ranging from the root, to the leaf. Two articles reported a specific antifungal activity against Candida albicans while the remaining one against Trichophyton rubrum. In conclusion, the root, leaf, branch, and flower extracts of orchid is potential antifungal resources.

Isolation and Characterization of Chalcone Synthase (CHS) Gene in Variegated-Flower of Dendrobium 'Enobi' and Phalaenopsis Hybrid Orchids

“…The DNA isolation method for purple flowers, which contain anthocyanin, has been modified because the anthocyanins contain polyphenols that can inhibit DNA amplification (Sityardi and Desrini 2021). CTAB method with modification in this research has been proven to produce a high quantity of DNA genome and PCR amplification based on the band thickness visualized on agarose gel electrophoresis (Figure 2 and 3).…”

“…The genome DNA was isolated based on Murray and Thompson's (1980) method, with modifications by adding 1% PVP to the CTAB to produce good and thick DNA genome bands from the flower sample. Modifications were made because the orchid contains polyphenols (Sityardi and Desrini 2021). Polyphenols can inhibit various processes as contaminants in DNA isolation (Nugroho et al 2015).…”