Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats

Abstract

Background: Peel is one of the major by-products in fruit processing industry. Fruit products (non-edible parts) are also considered as waste products and often discarded in the environment. Fruit peels are now serving as one of the primary sources for isolation and extraction of secondary metabolites in pharmaceutical industry. The present investigation was carried out to screen the phytochemical constituents and HPTLC analysis of peel extracts of Punica granatum and Citrus aurantifolia and their antidiabetic potential in alloxan-induced diabetic rats. Results: Among the different solvent extracts, methanol solvent extract was found to possess more amounts of secondary metabolites. In addition, HPTLC analysis of the plant samples revealed the presence of 13 peaks in both the plants by using gallic acid as marker. Different biochemical parameters such as blood glucose, cholesterol, protein, urea, creatinine, and triglycerides level were subjected for estimation by collecting the blood samples from the treated diabetic rats after 21 days. A sharp decline in blood glucose, cholesterol, triglycerides, creatinine, and urea level was noticed when methanolic extracts of Punica granatum and Citrus aurantifolia were given to experimental animals when compared with negative control. However, protein and weight of the animal were found to be enhanced when treated with methanolic extracts of both the plants. Conclusion: It can be concluded that fruit peels of both the plants exhibited antidiabetic potential on alloxaninduced diabetic rats which can be attributed to wide range of active pool of secondary metabolites. Further, screening and isolation of secondary metabolites along with their mode of action is required for effective use of plant-based drugs as antihyperglycemic agent.

Screening of Manilkara zapota (L) P. Royen stem bark ethanolic extract for in vitro α-glucosidase inhibition, preliminary antidiabetic effects, and improvement of diabetes and its complications in alloxan-induced diabetes in Wistar rats

“…dose (1 ml kg −1 ) of freshly prepared alloxan monohydrate (150 mg kg −1 ) dissolved in 0.9% saline was inoculated after light ether anesthesia. To avoid severe acute hypoglycemia, normal water fed was substituted with 5% glucose water for up to the next 48 h (Rees and Alcolado 2005;Ramya et al 2020;Ighodaro et al 2017). Fasting blood glucose levels were recorded after 72 h in all the alloxan monohydrate inoculated rats and rats with blood glucose levels ≥ 200 mg/dl were well-thought-out as diabetic (Chika and Bell 2010).…”

Bioactive Components and Their Activities from Different Parts of Citrus aurantifolia (Christm.) Swingle for Food Development

“…Antidiabetic An antidiabetic effect of C. aurantifolia bark extract has been reported. In a study by Ramya et al (2020) [121], using a methanolic extract of C. aurantifolia in vitro on rats, the alloxan-induced antidiabetic potential was shown. This activity is associated with the presence of secondary metabolites found in the peel of C. aurantifolia.…”

Characterization and Evaluation of Multiple Biological Activities of Silver Nanoparticles Fabricated from Dragon Tongue Bean Outer Peel Extract

“…Various research reports, that vegetable peels are having antidiabetic, cardio-protective, and antioxidant potentials. [35][36][37] Peels are the main portion of the vegetables and fruits. Generally, these peels are waste products and their disposal is challenging.…”