Potential of Photorhabdus temperata K122 bioinsecticide in protecting wheat flour against Ephestia kuehniella

Jallouli, Wafa; Abdelkefi-Mesrati, Lobna; Tounsi, Slim; Jaoua, Samir; Zouari, Nabil

doi:10.1016/j.jspr.2013.03.001

Cited by 15 publications

(16 citation statements)

References 29 publications

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“…The K122 strain was used for bioinsecticide production because of its high toxicity to the Lepidopterean insect larvae E. kuehniella. P. luminescens strain Q 167/2 is a non pathogenic bacterium, used as a negative control in the bioassay (Jallouli et al 2013).…”

Section: Methodsmentioning

confidence: 99%

“…Ephestia kuehniella is another target insect belonging to the order of Lepidotera. 100 % mortality of this insect larvae was reached after oral administration of broth medium at a concentration of 12×10 8 cells/mL (Jallouli et al 2013). Moreover, mortality of protonymph, deutonymh, adult males and adult females of the polyphagous pest Tetranychus urticae, belonging to the order of Trombidiformes was recorded after oral administration of P. temperata cell free supernatant (Eroglu et al 2019).…”

Section: Introductionmentioning

confidence: 94%

“…Samples collected after h of fermentation were subjected to determination of total cell count (Jallouli et al 2008) and insecticidal activity (Jallouli et al 2013). Total direct count was determined microscopically using Thoma counting chamber at 100-fold magnification.…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…P. temperata toxicity was demonstrated to be linearly related to biomass production during fermentation process in OM and wastewater used as an alternative medium (Jallouli et al 2013;Keskes et al 2020).…”

Section: Determination Of Specific Rates Of Cell Growth and Substratementioning

confidence: 99%

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Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

Keskes

Jallouli

Atitallah

et al. 2020

Preprint

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This study investigates the optimization of culture conditions for enhancing Photorhabdus temperata biopesticide production using wastewater (WS4) as a raw material. Box-Behnken design (BBD) was used to evaluate the effect of carbon to nitrogen ratio (C/N), NaCl concentration and inoculum size on Photorhabdus temperata biomass production and insecticidal activity. Modelling results suggest that the selected variables had contributed significantly to the responses. For enhanced biopesticide production the optimum operation conditions were as follow: inoculum size=4 %; C/N ratio=12.5 and sodium chloride concentration= 4 g/L for two responses. 1.95 and 2.75 folds improvement in oral toxicity and biomass production, respectively, were obtained when using the three variables at their optimum values. From batch fermentations carried out in the cost-effective medium developed in this study (WS4 I) and WS4 used as control, P. temperata kinetic parameters in term of biomass production and substrate consumption were modeled. The obtained results showed that the maximum specific growth rate was of 0.38 h-1 compared to 0.16 h-1 obtained in WS4. In addition, the efficiency of P. temperata to metabolize organic carbon was enhanced by optimizing culture conditions reaching 70 % instead of 47.2 % in the control fermentation. Under the optimized conditions, P. temperata cells showed higher specific consumption rate resulting in P. temperata toxin synthesis improvement.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Determination Of Specific Rates Of Cell Growth and Substratementioning

confidence: 99%

See 2 more Smart Citations

Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

Keskes

Jallouli

Atitallah

et al. 2020

Preprint

Self Cite

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show abstract

“…The Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), is also an economically important pest of stored products, attacking grains, dried fruits and nuts. Larvae not only cause direct damage by feeding, but their presence, frass production and webbing reduce product quality (Jallouli et al., ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a Serratia marcescens with insecticidal activity from Polyphylla olivieri (Col.: Scarabaeidae)

Bidari

Shams-Bakhsh

Mehrabadi

2017

J Applied Entomology

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Members of the genus Serratia are known for their abilities to infect insects. In this study, a red-pigmented S. marcescens was isolated and characterized from the infected larvae of Polyphylla olivieri using bacterial cultivation, phylogenetic analysis as well as bioassays against larvae of the two insect pests, Plodia interpunctella and Ephestia kuehniella. Comparative 16S rRNA and groEL gene sequence BLAST analyses strongly suggested that the isolated strain should be placed in the genus Serratia, sharing high sequence similarities with several strain of S. marcescens associated with insects.Phylogenetic analysis placed the isolated bacterium with other S. marcescens bacteria in a clade with high bootstrapping values. To assess pathogenicity of the S. marcescens isolate, the bacterial cells were either injected into the haemolymph of the fifth-instar larvae or added to the diets of insects. Survival curves of the control insects and those challenged with six different concentrations of S. marcescens showed that the S. marcescens isolate significantly reduced survival rates of the larvae. The LC 50 s of the bacterium on P. interpunctella and E. kuehniella were 1992.26 and 1.09 × 10 4 (CFU/μl) for injection bioassays at 6 h post-injection, and 4.48 × 10 4 and 1.96 × 10 5 (CFU/10 μl)for feeding bioassays at 24 h post-feeding, respectively. Injection of the bacterial culture supernatant into the larvae led to continuous bleeding from the site of injection, while injection of heat-treated culture supernatant of the bacterium did not cause continuous bleeding. Together, our results showed the possibility of using this S. marcescens isolate in microbial control of the insect pests after addressing the safety concerns. Moreover, it might be considered as a source of useful bioactive molecules and genes with application in insect control and biotechnology via developing insectresistant plants.

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Effects of Entomopathogenic Nematodes and Symbiotic Bacteria on Non-target Arthropods

Sandhi

Reddy

2019

Sustainability in Plant and Crop Protection

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Potential of Photorhabdus temperata K122 bioinsecticide in protecting wheat flour against Ephestia kuehniella

Cited by 15 publications

References 29 publications

Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

Isolation and characterization of a Serratia marcescens with insecticidal activity from Polyphylla olivieri (Col.: Scarabaeidae)

Effects of Entomopathogenic Nematodes and Symbiotic Bacteria on Non-target Arthropods

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