Potential of Phylloplane Fungi from Mangrove Plant (Rhizophora apiculata Blume) as Biological Control Agents against Fusarium oxysporum f. sp. cubense in Banana Plant (Musa acuminata L.)

Shara, Melya; Basyuni, Mohammad; Hasanuddin,

doi:10.3390/f14020167

Cited by 4 publications

(1 citation statement)

References 49 publications

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“…Hal ini mendorong penelitian baru untuk menemukan kapang endofit mangrove sebagai sumber antibiotik untuk menghindari eksploitasi berlebihan dan menjaga kelimpahan tumbuhan mangrove. Kapang endofit mangrove menghasilkan senyawa metabolit sekunder yang serupa dengan metabolit sekunder inangnya (Shara et al, 2023). Penelitian terdahulu menemukan bahwa kapang endofit mangrove memiliki kemampuan aktivitas antibakteri terhadap patogen Staphylococcus aureus, S. epidermidis, dan Bacillus sibtilus (Handayani et al, 2020).…”

Section: Pendahuluanunclassified

Potensi Metabolit Kapang Endofit Mangrove Rhizophora apiculata sebagai Anti-Staphylococcus epidermidis

Limbong,

Setyati,

Sedjati

et al. 2023

J. Kel. Trop.

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Mangrove association molds are known to produce secondary metabolite compounds that have antibacterial activity. The purpose of this study was to obtain anti-Staphylococcus epidermidis metabolites produced by the endophytic mold of mangrove Rhizophora apiculate. The screening process is carried out in exploratory descriptive research. The stages of this research are sampling, mold isolation and purification, and macroscopic characterization. Initial screening of antibacterial activity is carried out by the agar plug method. Isolates that produce antibacterial metabolites are molecularly identified. The isolate was then cultured with Potato Dextrose Agar (PDA) media. The producing antibacterial metabolites were extracted using two solvents of different polarities (ethyl acetate and methanol) by stratified maceration method. Antibacterial metabolites are then tested to determine the concentration of extracts required to inhibit (Minimum Inhibitory Concentration (MIC) and kill bacterial growth (Minimum Bactericidal Concentration / MBC). The results of this study showed that there is one species of mangrove endophytic mold Rhizophora apiculata that can produce antibacterial metabolites against S. epidermidis, namely Diaporthe sennae. These antibacterial metabolites are present in ethyl acetate extract. The resulting bioactivity based on the ability to inhibit bacterial growth can be seen from the MIC value of 2.5 mg / mL and the ability to kill bacteria with an MBC value of 5 mg / mL. The mold metabolite Diaporthe sennae could potentially be developed as an anti-Staphylococcus epidermidis Kapang asosiasi mangrove diketahui menghasilkan senyawa metabolit sekunder yang memiliki aktivitas antibakteri. Tujuan penelitian ini adalah untuk mendapatkan metabolit anti-Staphylococcus epidermidis yang dihasilkan oleh kapang endofit mangrove Rhizophora apiculate. Proses skrining dilakukan secara diskriptif eksploratif. Tahapan penelitian ini adalah pengambilan sampel, isolasi dan purifikasi kapang, serta karakterisasi makroskopis. Skrining awal aktivitas antibakteri dilakukan dengan metode agar plug. Isolat yang menghasilkan metabolit antibakteri diidentifikasi secara molekuler. Isolat tersebut selanjutnya dikultur dengan media Potato Dextrose Agar (PDA). Metabolit antibakteri yang dihasilkan diekstraksi menggunakan dua pelarut yang berbeda polaritas (etil asetat dan metanol) dengan metode maserasi bertingkat. Metabolit antibakteri kemudian diuji untuk menentukan konsentrasi ekstrak yang diperlukan untuk menghambat (Minimum Inhibitory Concentration /MIC) dan membunuh pertumbuhan bakteri (Minimum Bactericidal Concentration /MBC). Hasil penelitian ini menunjukkan bahwa terdapat satu spesies kapang endofit mangrove Rhizophora apiculata yang yang dapat menghasilkan metabolit antibakteri melawan S. epidermidis, yaitu Diaporthe sennae. Metabolit antibakteri tersebut terdapat dalam ekstrak etil asetat. Bioaktivitas yang dihasilkan berdasarkan kemampuan menghambat pertumbuhan bakteri terlihat dari nilai MIC sebesar 2,5 mg/mL dan kemampuan membunuh bakteri dengan nilai MBC sebesar 5 mg/mL. Metabolit kapang Diaporthe sennae berpotensi dikembangkan sebagai anti-Staphylococcus epidermidis

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Section: Pendahuluanunclassified

Potensi Metabolit Kapang Endofit Mangrove Rhizophora apiculata sebagai Anti-Staphylococcus epidermidis

Limbong,

Setyati,

Sedjati

et al. 2023

J. Kel. Trop.

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Phylloplane as Fungi Habitat

Tsarelunga,

Blagoveschenskaya

2024

Biol Bull Rev

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Mangrove Ecosystem Components and Benefits

El-Sayed,

Abdrabo,

Hamed

2023

Marine Ecology: Current and Future Developments

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There is a wide variety of plant, animal, and microbial life in mangrove forests because of their location at the boundary between terrestrial and marine environments. Because of their central role in the development and upkeep of the mangrove ecosystem, microbes also serve as a useful and significant source of biotechnologically engineered materials. Microbes are essential to the health of the mangrove ecosystem's productivity by aiding in the decomposition and mineralization of leaf litter at a number of different phases of the process. They are capable of recycling nutrients; they can generate or consume gases affecting the global climate; they can remove contaminants; they can process anthropogenic trash. Mangrove environment microorganisms provide a large supply of antimicrobial substances and also create a broad spectrum of major health-boosting chemicals such as enzymes, antitumors, insecticides and immune modulators. However, unlike other ecosystems, mangrove ecosystems have never had their microbial diversity described. Despite the rich diversity of microbiological conditions in mangrove ecosystems, only around 5 percent of species have been classified, and many of them remain enigmas in terms of their ecological importance and practical use. Microbial diversity must be fully utilised to reach its potential, and modern molecular biology and genetics technologies show considerable promise. This Chapter, therefore, attempts to examine and analyze the microbial diversity of mangrove ecosystems in many aspects, such as agricultural, pharmaceutical, industrial, environmental, and medical possibilities.

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Potential of Phylloplane Fungi from Mangrove Plant (Rhizophora apiculata Blume) as Biological Control Agents against Fusarium oxysporum f. sp. cubense in Banana Plant (Musa acuminata L.)

Cited by 4 publications

References 49 publications

Potensi Metabolit Kapang Endofit Mangrove Rhizophora apiculata sebagai Anti-Staphylococcus epidermidis

Potensi Metabolit Kapang Endofit Mangrove Rhizophora apiculata sebagai Anti-Staphylococcus epidermidis

Phylloplane as Fungi Habitat

Mangrove Ecosystem Components and Benefits

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