Potential of PM-selected components to induce oxidative stress and root system alteration in a plant model organism

Piacentini, Diego; Falasca, Giuseppina; Canepari, Silvia; Massimi, Lorenzo

doi:10.1016/j.envint.2019.105094

Cited by 26 publications

(28 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three persistent DEGs enriched in acetyl-CoA biosynthetic process were homologs of Pyruvate dehydrogenase E1, ALPHA in A. thaliana affecting polar auxin transport during root development (Ohbayashi and Huang 2019). Oxidative stress response is a general response of living organisms to biotic or abiotic stress (Piacentini et al 2019). Ten out of the 14 persistent DEGs (Table S9) enriched in oxidative stress encoded 10 proteins PEROXIDASE 34 (PRX34), CYP709B3, CYP87A3, CYP78A6, PEROXIDASE 3 (PER3), PER34, PER44, PER71, CATALASE-2 (CAT2), and ALPHA-DIOXYGENASE 1 (DOX1), and these genes have been reported to act as regulators in roots development in Arabidopsis (Takei et al 2004;Kim et al 2010;Renault et al 2013;Estornell et al 2018;Renard et al 2020).…”

Section: Possible Regulatory Pathways Of Persistent and Stage-specific Genetic Factors Related To Root Developmentmentioning

confidence: 99%

Genome-Wide Association Study and Transcriptome Analysis Reveal Key Genes Affecting Root Growth Dynamics In Rapeseed

Wang

Kuang

et al. 2021

Preprint

View full text Add to dashboard Cite

Root growth is a dynamic process mediated by interactive genetic factors and environmental cues. Seven root-related traits and two shoot-related traits in 280 Brassica napus accessions at five continuous vegetative stages were measured to establish the genetic basis of root growth in rapeseed. The persistent and stage-specific genetic mechanisms were revealed by root dynamic analysis. Sixteen persistent and 32 stage-specific quantitative trait loci (QTL) clusters were identified through genome-wide association study (GWAS). Root samples with contrasting (slow and fast) growth rates throughout investigated stages and those with obvious stage-specific changes in growth rates were subjected to transcriptome analysis. The former identified 367 differentially expressed genes (DEGs) with persistently differential expressions throughout root development that were significantly enriched in GO terms of energy metabolism and response to biotic or abiotic stress, whereas the later identified 485 stage-specific DEGs with different expressions at corresponding stage that were enriched in GO terms of nitrogen metabolism. By integrating GWAS, weighted gene co-expression network analysis (WGCNA), and differential expression analysis, four and eight candidate genes less than 100 kb away from peak SNPs of QTL clusters were identified as key persistent and stage-specific genetic factors, respectively. The homologs of 3 genes ( BnaA03g52990D , BnaA06g37280D, and BnaA09g07580D ) out of 12 candidate genes have been reported to regulate root development in previous studies. Our results provide new insights into the temporal genetic mechanisms of root growth by identifying key candidate QTL/genes in rapeseed.

show abstract

Section: Possible Regulatory Pathways Of Persistent and Stage-specific Genetic Factors Related To Root Developmentmentioning

confidence: 99%

Genome-Wide Association Study and Transcriptome Analysis Reveal Key Genes Affecting Root Growth Dynamics In Rapeseed

Wang

Kuang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The localization and the geographical coordinates of the 23 PM 10 sampling sites as well as the characteristics of the used HSRS are deeply described in Massimi et al 2017 andMassimi et al 2019 [11,31]. The collected monthly PM 10 membrane filters were subjected to a chemical fractionation procedure, previously optimized and validated [37,38], useful for separating the water-soluble and insoluble fraction of each PM 10 elemental component, thus increasing its selectivity as a source tracer [11,39,40]. Firstly, after removing the supporting polymethylpentene ring from each membrane filter, PM 10 filters were extracted in 10 mL of deionized water for 30 min at 25 • C by using an ultrasonic bath (Proclean 10.0 ultrasonic cleaner, Ulsonix, Germany) and were then filtered on cellulose nitrate filters (0.45 µm pore size, Merck Millipore Ltd., Billerica, MA, USA).…”

Section: Pm 10 Samples Collection and Preparationmentioning

confidence: 99%

Evaluation of the Efficiency of Arundo donax L. Leaves as Biomonitors for Atmospheric Element Concentrations in an Urban and Industrial Area of Central Italy

et al. 2020

Self Cite

View full text Add to dashboard Cite

Washed and unwashed Arundo donax L. (A. donax) leaves were analyzed for elements, and results were compared with element concentrations detected in river water and particulate matter (PM) Samples were collected along a river in an urban and industrial hot spot of Central Italy, where element concentrations show relevant spatial gradients both in air and river water. The aim of this study is to identify the role of the two environmental matrices on leaves composition. Element concentrations of washed and unwashed leaves were compared to differentiate between the superficial deposition and the uptake into leaf tissues of elements. Water-soluble and -insoluble element concentrations were measured in PM10 samples collected on membrane filters by using innovative high spatial resolution samplers. The comparison among leaf and atmospheric concentrations of PM10 elements showed a similar trend for Ni, Mo, Cr, Ti, and Fe, which are reliable tracers of the PM10 contribution by steel plant and vehicular traffic. Soluble species appeared to be mainly bounded into leaf tissues, while insoluble species were deposited on their surface. On the other hand, element concentrations detected in washed A. donax leaves were poorly correlated with those measured in river water samples. The obtained results proved that A. donax leaves can be used as reliable biomonitors for the evaluation of the atmospheric concentrations of some PM10 elemental components.

show abstract

“…Coke dust contains very high concentrations of organic toxic species [46] and was taken from the ground near a coal park. Calcitic soil dust, one of the major natural compounds of PM [20], was collected in rural areas around the city of Rome. Incinerator dust was sampled by a fine-mesh filter placed in a waste-to-energy plant chimney in Northern Italy.…”

Section: Collection and Chemical Characterization Of Samplesmentioning

confidence: 99%

“…In fact, it is increasingly recognized that this PM property is more closely associated with adverse The aim of this work was to evaluate the applicability of the DPPH assay to PM for measuring reducing potential (RP) due to the presence of antioxidant species which could, in the future, integrate the information about redox properties of PM obtained by OP assays. In this work, the DPPH assay was applied to seven types of widespread components of PM produced by specific emission sources and characterized by very different chemical compositions [19,20] (i.e., urban dust certified for its elemental content, UD; brake dust, BD; Saharan dust, SD; coke dust C; calcitic soil dust, CSD; incinerator dust, ID; and certified diesel particulate matter, D). Moreover, the DPPH assay was applied to PM 2.5 field samples collected during a short monitoring campaign.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the combined application of different acellular methods on the same samples is strongly suggested. Although some recent studies were focused on the in vivo evaluation of PM's negative impacts on living organisms [18][19][20], knowledge about the relationships between OP and PM's toxicological effects still presents some important gaps [6,20,21].Other still unresolved issues are related to the assay's design and the influence of the operative conditions by which OP tests are performed. In fact, over recent years, many researchers underlined how test conditions could influence collected data.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Application of DPPH Assay for Assessment of Particulate Matter Reducing Properties

et al. 2019

Self Cite

View full text Add to dashboard Cite

Different acellular assays were developed to measure particulate matter’s (PM) oxidative potential (OP), a metric used to predict the ability of PM in generating oxidative stress in living organisms. However, there are still fundamental open issues regarding the complex redox equilibria among the involved species which could include reducing compounds. The aim of this study was the pilot application of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to PM in order to evaluate the presence of reducing species. The assay, commonly applied to biological matrices, was adapted to PM and showed good analytical performances. It allowed the analysis of conventional 24 h airborne PM samples with suitable sensitivity and good repeatability of the measurements. The assay was applied to seven samples representing possible PM contributes (certified urban dust NIST1648a; brake dust; Saharan dust; coke dust; calcitic soil dust; incinerator dust; and diesel particulate matter certified material NIST1650b) and to PM2.5 field filters. The same samples were also analyzed for elements. Preliminary results indicated that the assay gave a linear response and that detectable amounts of reducing species were present in PM samples. The combined application of DPPH and conventional OP assays could then permit, in the future, to gain more knowledge about the reaction and/or competition between oxidative and reducing processes.

show abstract

Potential of PM-selected components to induce oxidative stress and root system alteration in a plant model organism

Cited by 26 publications

References 69 publications

Genome-Wide Association Study and Transcriptome Analysis Reveal Key Genes Affecting Root Growth Dynamics In Rapeseed

Genome-Wide Association Study and Transcriptome Analysis Reveal Key Genes Affecting Root Growth Dynamics In Rapeseed

Evaluation of the Efficiency of Arundo donax L. Leaves as Biomonitors for Atmospheric Element Concentrations in an Urban and Industrial Area of Central Italy

Application of DPPH Assay for Assessment of Particulate Matter Reducing Properties

Contact Info

Product

Resources

About