Potential of polyE-323 coated capillaries for capillary electrophoresis of lipids

Martma, Kert; Lindenburg, Petrus W.; Habicht, Kaia-Liisa; Vulla, Kaspar; Resik, Kristiin; Kuut, Gunnar; Shimmo, Ruth

doi:10.1016/j.chroma.2013.08.054

Cited by 3 publications

(1 citation statement)

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“…Cationic coatings have a certain advantage in fast adsorption to a negatively charged fused silica surface and they usually maintain a stable EOF over a wide range of pH values. Some of the very recent works with cationic coatings include applications of polymers for hindering proteins from adsorbing to the capillary wall , in protein tryptic digest separations , in lipid analysis , and in their combination with negatively charged layers to form more stable multilayered coatings . It is also important to consider the polymer charge density because a too fast EOF may prevent good identification of migrated compounds in complex mixtures .…”

Section: Introductionmentioning

confidence: 99%

Novel cationic polyelectrolyte coatings for capillary electrophoresis

et al. 2015

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The use of bare fused silica capillary in CE can sometimes be inconvenient due to undesirable effects including adsorption of sample or instability of the EOF. This can often be avoided by coating the inner surface of the capillary. In this work, we present and characterize two novel polyelectrolyte coatings (PECs) poly(2-(methacryloyloxy)ethyl trimethylammonium iodide) (PMOTAI) and poly(3-methyl-1-(4-vinylbenzyl)-imidazolium chloride) (PIL-1) for CE. The coated capillaries were studied using a series of aqueous buffers of varying pH, ionic strength, and composition. Our results show that the investigated polyelectrolytes are usable as semi-permanent (physically adsorbed) coatings with at least five runs stability before a short coating regeneration is necessary. Both PECs showed a considerably decreased stability at pH 11.0. The EOF was higher using Good's buffers than with sodium phosphate buffer at the same pH and ionic strength. The thickness of the PEC layers studied by quartz crystal microbalance was 0.83 and 0.52 nm for PMOTAI and PIL-1, respectively. The hydrophobicity of the PEC layers was determined by analysis of a homologous series of alkyl benzoates and expressed as the distribution constants. Our result demonstrates that both PECs had comparable hydrophobicity, which enabled separation of compounds with log Po/w > 2. The ability to separate cationic drugs was shown with β-blockers, compounds often misused in doping. Both coatings were also able to separate hydrolysis products of the ionic liquid 1,5-diazabicyclo[4.3.0]non-5-ene acetate at highly acidic conditions, where bare fused silica capillaries failed to accomplish the separation.

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Section: Introductionmentioning

confidence: 99%

Novel cationic polyelectrolyte coatings for capillary electrophoresis

et al. 2015

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Capillary Electrophoresis–Mass Spectrometry for Lipid Analysis

Lindenburg

Vulla

Köhler

et al. 2018

Capillary Electrophoresis – Mass Spectrometry for Metabolomics

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Capillary electrophoresis coupled to mass spectrometry (CE-MS) is a powerful technique that enables the selective and sensitive analysis of a wide variety of compounds in a large number of biological matrices. CE-MS also offers excellent possibilities for the analysis of minute samples, leading to very limited waste production. All these advantages render CE-MS an attractive technique for the analysis of lipids, a class of hydrophobic metabolites showing a wide physico-chemical diversity and having gained significant attention over the last few years in clinical research due to their key roles in multiple pathophysiological conditions. This chapter discusses the potential of CE-MS for lipid analysis, providing an overview of developments and applications reported over the past 20 years (i.e., since 1997). It is structured according to distinct lipid classes, i.e. phospholipids, glycolipids, acylcarnitines, surfactants, and fatty acids. Experimental conditions, including CE mode and MS type and details about the hyphenation of CE to MS are reported. Extra attention is paid to the use of capillary coatings to improve lipid separation. We introduce the use of M7C4I (ω-iodoalkylammonium salt) as a capillary coating agent for the analysis of apolar compounds and demonstrate its potential for the analysis of lipids in a complex biological sample.

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