Potential of prodendronic polyamines with modulated segmental charge density as novel coating for fast and efficient analysis of peptides and basic proteins by CE and CE‐MS

Abstract

In this work, the suitability of a new polymer family has been investigated as capillary coatings for the analysis of peptides and basic proteins by CE. This polymer family has been designed to minimize or completely prevent protein-capillary wall interactions and to modify the EOF. These coating materials are linear polymeric chains bearing as side cationizable moiety a dentronic triamine derived from N,N,N',N'-tetraethyldiethylenetriamine (TEDETA), which is linked to the backbone through a spacer (unit labeled as TEDETAMA). Four different polymers have been prepared and evaluated: a homopolymer which comprised only of those cationizable repetitive units of TEDETAMA, and three copolymers that randomly incorporate TEDETAMA together with neutral hydrosoluble units of N-(2-hydroxypropyl) methacrylamide (HPMA) at different molar percentages (25:75, 50:50 and 75:25). It has been demonstrated that the composition of the copolymers influences the EOF and therefore the separation of the investigated biopolymers. Among the novel polymers studied, poly-(TEDETAMA-co-HPMA) 50:50 copolymer was successfully applied as coating material of the inner capillary surface in CE-UV and CE-MS, providing EOF reversing together with fast and efficient baseline separation of peptides and basic proteins. Finally, the feasibility of the polymer-coated capillary was shown through the analysis of lysozyme in a cheese sample.

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

“…A new polymer family, prodendronic polyamines with modulated segmental charge density, providing reverse EOF with variable velocity within wide pH range 2–10, was shown as a suitable capillary coating for fast and efficient separations of basic peptides and proteins by CE‐UV and CE‐MS . High efficient separation of lysozyme, cytochrome c, and their tryptic digests were achieved within ca.…”

Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip

“…The separation performance of CE can be notably impacted by the occurrence of electroosmotic flow (EOF) [21,22]. To reduce the adverse effects of EOF, a promising strategy involves the chemical modification of microchannel surfaces through coating techniques [23]. We investigated the effect of the coating on the electrophoretic separation performance of DNA.…”

Recent advances in microscale separation

“…Till now, a variety of different species have been employed as coatings materials or additives for microscale separation, ranging from small molecules to larger molecules, including ILs , NPs , surfactant , chitin , cellulose , poly(dopamine) , polymerized phospholipid , polyethylene glycol (PEG) , polyvinyl alcohol (PVA) , polycationic polymers , copolymer , and so on (see Table ).…”