Potential of some endophytic bacteria in biological control of root-knot nematode Meloidogyne incognita

Moslehi, Shalaleh; Pourmehr, Salar; Shirzad, Akbar; Khakvar, Reza

doi:10.1186/s41938-021-00396-4

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…A. chroococcum, a nitrogen fixing bacteria and B. weidmannii NRC211 singly or combined in soil naturally infested with M. javanica recorded variable significant reductions in nematode developmental parameters with considerable increase in yield production and oil content. These data agree with previous studies on beneficial microorganisms in controlling plant pathogens and enhancing crop production (Moslehi et al 2021). The nematicidal activity of these bacterial agents might be due to secretion of antimicrobial compounds.…”

Section: Discussionsupporting

confidence: 92%

“…This could be attributed to the fact that NPK contains a mixture of 4 microbial species viz: B. polymyxa, B. megaterium, B. circulance and Pseudomonas sp. Each one of these microbial strains has its own nematicidal mechanisms as well as its plant growth promoting effects as previously mentioned (Moslehi et al 2021). Moreover, A. chroococcum either single or combined with NPK treatments showed the greatest increase in number of pods, in addition NPK + Az combined treatment exhibited significant increase in pods weight, weight of 200 seeds, peanut crop production and oil content.…”

Section: Discussionmentioning

confidence: 58%

“…In a study on nematicidal effects of some strains of B. polymyxa, B. megaterium and B. circulance, the results indicated that the bacterial biofertilizers were promising double-purpose microorganisms for mobilizing of soil nutrients and for the biological control of M. incognita (Moslehi et al 2021). Ann (2013), reported that antimicrobial metabolites and enzymes from Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

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Bio-fertilizers’ protocol for controlling root knot nematode Meloidogyne javanica infecting peanut fields

Osman¹,

Ameen²,

Mohamed³

et al. 2021

Egypt J Biol Pest Control

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Background Plant parasitic nematodes create serious threat to crop production. In Egypt root knot nematode, Meloidogyne spp. has been considered to be a limiting factor in the production of most crops of which the Peanut (Arachis hypogaea L.) is an important legume and oil crop. Therefore, management of root knot nematodes Meloidogyne spp. is an obligatory challenge. Microbial organisms are extensively used as eco-friendly tools for controlling plant parasitic nematodes as alternative to chemical nematicides. The effectiveness of the commercial bacterial bio-fertilizers NPK containing Bacillus polymyxa, B. circulance, B. megaterium, Pseudomans spp.; the nitrogen fixative bacteria Azotobacter chroocoocum and the bacterial isolate NRC211 were evaluated against the root knot nematode, Meloidogyne javanica infecting peanut plants under field conditions. Identification of the bacterial isolate was made through PCR amplification and sequencing of 16S rDNA gene. Results Sequencing of 16S rDNA gene revealed that the bacterial isolate NRC211 had 100% similarity with Bacillus wiedmannii strain FSL W8-0169 16S ribosomal RNA. This Bacillus was recorded for the first time under accession number LC626774 on GenBank data base as B. wiedmannii NRC211. Recorded data revealed that all the tested treatments whether single or combined in soil naturally infested with M. javanica, resulted in variable significant reduction in the nematode reproductive parameters with a considerable increase in crop production and oil content of peanut plant. These results were improved by increasing the frequency of application of the bio-agents. In this respect the repeated combined treatment of A. chroococcum and B. wiedmannii NRC211 treatment overwhelmed all other treatments in decreasing nematode reproductive parameters with percentage reductions of 94.8, 79.0 and 80.1% in M. javanica juveniles in soil, galls and egg masses, respectively. This was associated with slight increase in peanut oil content than the untreated control. The repeated combined treatment of NPK plus A. chroococcum produced the highest increase 608.7%, and 72.7% in crop production and plant growth parameters, respectively than the control. While, the oil content in this treatment was increased up to 47.4 g/kg. Conclusion It was concluded that B. wiedmanni NRC211 is an eco-friendly bio agent that can be applied with other commercial microbial bio-fertilizers in bio-integrating programs for controlling M. javanica infecting peanut plants.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Bio-fertilizers’ protocol for controlling root knot nematode Meloidogyne javanica infecting peanut fields

Osman¹,

Ameen²,

Mohamed³

et al. 2021

Egypt J Biol Pest Control

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“…Several genera were predominant and determined the dissimilarities among the compartments (Figures S7-S10). Many of the biomarkers, including for Bacillus, Pseudomonas, Agrobacterium, Gemmatimonas, Enterobacter, Streptomyces, Devosia, Ensifer, Acinetobacter, Halomonas, Brevundimonas, Mesorhizobium, and Burkholderia, are known as beneficial microbes for plants, and these microbes may help to maintain plant hormone balance, control root development, facilitate nutrition acquisition, and prevent disease in the plant host [42,44,[51][52][53][54][55]. Several studies have reported that Streptomycetes are abundant inside of the roots of Arabidopsis thaliana [56] where they can have beneficial effects on growth [57] or protect plants against biotic and abiotic stressors [58,59].…”

Section: Root Compartment-specific Bacterial Biomarkers Varied With G...mentioning

confidence: 99%

Differed Growth Stage Dynamics of Root-Associated Bacterial and Fungal Community Structure Associated with Halophytic Plant Lycium ruthenicum

Yuan

et al. 2022

Microorganisms

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Lycium ruthenicum, a halophytic shrub, has been used to remediate saline soils in northwest China. However, little is known about its root-associated microbial community and how it may be affected by the plant’s growth cycle. In this study, we investigate the microbial community structure of L. ruthenicum by examining three root compartments (rhizosphere, rhizoplane, and endosphere) during four growth stages (vegetative, flowering, fruiting, and senescence). The microbial community diversity and composition were determined by Illumina MiSeq sequencing of the 16S V3–V4 and 18S ITS regions. Proteobacteria, Actinobacteria, Bacteroidetes, Planctomycetes, and Acidobacteria were the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the most dominant fungal phyla. The alpha diversity of the bacterial communities was highest in the rhizosphere and decreased from the rhizosphere to the endosphere compartments; the fungal communities did not show a consistent trend. The rhizosphere, rhizoplane, and endosphere had distinct bacterial community structures among the three root compartments and from the bulk soil. Additionally, PERMANOVA indicated that the effect of rhizocompartments explained a large proportion of the total community variation. Differential and biomarker analysis not only revealed that each compartment had unique biomarkers and was enriched for specific bacteria, but also that the biomarkers changed with the plant growth cycle. Fungi were also affected by the rhizocompartment, but to a much less so than bacteria, with significant differences in the community composition along the root compartments observed only during the vegetative and flowering stages. Instead, the growth stages appear to account for most of the fungal community variation as demonstrated by PCoA and NMDS, and supported by differential and biomarker analysis, which revealed that the fungal community composition in the rhizosphere and endosphere were dynamic in response to the growth stage. Many enriched OTUs or biomarkers that were identified in the root compartments were potentially beneficial to the plant, meanwhile, some harmful OTUs were excluded from the root, implying that the host plant can select for beneficial bacteria and fungi, which can promote plant growth or increase salt tolerance. In conclusion, the root compartment and growth stage were both determinant factors in structuring the microbial communities of L. ruthenicum, but the effects were different in bacteria and fungi, suggesting that bacterial and fungal community structures respond differently to these growth factors.

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“…The strain AzBw1 was introduced in an attempt to screen rhizobacteria based on their ability to control RKN of tomato plant (Moslehi et al 2021). This strain outperformed other strains such that in greenhouse conditions, root knots and egg mass development was considerably lower (60-70 percent) in AzBw1-treated plants in comparison to mock-treated plants.…”

Section: Introductionmentioning

confidence: 99%

Possible mechanisms of action of Bacillus wiedmannii AzBw1, a biocontrol agent of the root-knot nematode, Meloidogyne arenaria

Fallahzadeh-Mamaghani

Shahbazi-Ezmareh

Shirzad

et al. 2023

Egypt J Biol Pest Control

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Background With increased environmental concerns and restrictions of chemical control, the importance of other eco-friendly strategies for management of the nematodes is being substantially grown nowadays. One of the most well-known strategies that have attracted the attentions is biological control of these deleterious agents. In our previous study (Moslehi et al. in Egypt J Biol Pest Control 31:1–11, 2021), Bacillus wiedmannii AzBw1 was introduced as a robust antagonistic agent against root-knot nematode Meloidogyne arenaria. Present study addressed the possible mechanisms of action of this strain. Results Based on quantitative bioassays it was shown that the strain AzBw1 is able to produce considerable amount of siderophore, protease, and chitinase. In an in vitro assay conducted by bi-plate Petri dishes, it was shown that hatching of the nematode eggs, subjected to bacterial volatile compounds (BVCs) was 34% lower than those of mock-treated control eggs. On the other hand, mortality of BVC-treated juveniles was 33.5% higher than those of mock-treated control juveniles. The secretory proteins from the medium culture of strain AzBw1 were precipitated and fractionated by anion exchange chromatography (AEC). Fractions from AEC were checked for hydrolytic activity and nematicidal effect. It was found that the fractions with the highest protease activity have a strong nematicidal effect. In contrast, significant nematicidal effect in the fraction with Chitinase activity was detected. Conclusion The results suggested that protease activity played a key role in strain AzBw1 antagonism against root-knot nematode, M. arenaria. Finally, nonvolatile organic compounds were also extracted from the medium culture after removing secretory proteins and enzymes. Obtained results showed that these metabolites had also a strong anti-nematode effect.

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Potential of some endophytic bacteria in biological control of root-knot nematode Meloidogyne incognita

Cited by 10 publications

References 23 publications

Bio-fertilizers’ protocol for controlling root knot nematode Meloidogyne javanica infecting peanut fields

Bio-fertilizers’ protocol for controlling root knot nematode Meloidogyne javanica infecting peanut fields

Differed Growth Stage Dynamics of Root-Associated Bacterial and Fungal Community Structure Associated with Halophytic Plant Lycium ruthenicum

Possible mechanisms of action of Bacillus wiedmannii AzBw1, a biocontrol agent of the root-knot nematode, Meloidogyne arenaria

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