Potential of Streptomyces sp. strain AGS-58 in controlling anthracnose-causing Colletotrichum siamense from post-harvest mango fruits

Evangelista-Martínez, Zahaed; Ek-Cen, Abigail; Torres-Calzada, Claudia; Uc-Várguez, Alberto

doi:10.1007/s42161-022-01104-3

Cited by 10 publications

(4 citation statements)

References 54 publications

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“…This research confirmed the potential of eco-friendly microbial fungicides based on Streptomyces as alternatives to synthetic fungicides associated with various environmental and health issues. Several microbial fungicides based on Streptomyces strains are commercially available , such as AGBIO’s MYCOSTOP based on S. griseoviridis K61 producing the polyene macrolide candicidin, and NOVOZYMES’ ACTINOVATR based on S. lydicus WYEC108 producing the polyene macrolide natamycin 30 . Furthermore, ongoing research continue to confirm the potential of Streptomyces isolates as putative bio-fungicides.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Park,

Kang,

Choi

et al. 2024

Sci Rep

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Soil microorganisms with diverse bioactive compounds such as Streptomyces are appreciated as valuable resources for the discovery of eco-friendly fungicides. This study isolated a novel Streptomyces from soil samples collected in the organic green tea fields in South Korea. The isolation process involved antifungal activity screening around 2400 culture extracts, revealing a strain designated as S. collinus Inha504 with remarkable antifungal activity against diverse phytopathogenic fungi. S. collinus Inha504 not only inhibited seven phytopathogenic fungi including Fusarium oxysporum and Aspergillus niger in bioassays and but also showed a control effect against F. oxysporum infected red pepper, strawberry, and tomato in the in vivo pot test. Genome mining of S. collinus Inha504 revealed the presence of the biosynthetic gene cluster (BGC) in the chromosome encoding a polyene macrolide which is highly homologous to the lucensomycin (LCM), a compound known for effective in crop disease control. Through genetic confirmation and bioassays, the antifungal activity of S. collinus Inha504 was attributed to the presence of LCM BGC in the chromosome. These results could serve as an effective strategy to select novel Streptomyces strains with valuable biological activity through bioassay-based screening and identify biosynthetic gene clusters responsible for the metabolites using genome mining approach.

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Section: Discussionmentioning

confidence: 99%

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Park,

Kang,

Choi

et al. 2024

Sci Rep

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“…More recently, a strain of Streptomyces was isolated and found to show strong antifungal activity and produce various antifungal compounds against Colletotrichum fragariae , which causes postharvest decay of strawberries, as treatment of the fruits resulted in a reduced decay rate of strawberries and maintained fruit quality ( 35 ). Another strain of Streptomyces was found to show efficient control comparable to that of the chemical fungicide Benlate against Colletotrichum siamense causing anthracnose on mango fruits ( 36 ). A strain of Burkholderia was also reported to have antagonistic activity, reducing mycelial growth and conidial germination against Colletotrichum orbiculare , which causes anthracnose in cucumbers and has been suggested as a promising biocontrol agent ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Taxonomy-guided selection of Paraburkholderia busanensis sp. nov.: a versatile biocontrol agent with mycophagy against Colletotrichum scovillei causing pepper anthracnose

Mannaa,

Han,

Jeong

et al. 2023

Microbiol Spectr

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This study introduces a novel approach based on the taxonomy-guided selection of bacterial biocontrol agents from a known beneficial taxonomic group. Following 16S rRNA screening, we focused on the genus Paraburkholderia , which harbors strains with large genomes and versatile benefits to plants. A strain designated P39 was selected, identified, and characterized for its biocontrol activity against Colletotrichum scovillei . Strain P39 exhibited antagonism against C. scovillei by producing compounds, including volatiles, with antifungal activity, both in vitro and on pepper fruits. Genomic, physiological, and biochemical analyses revealed that the selected strain represents a novel species, named Paraburkholderia busanensis . Genomic analyses provided insights into the fitness and biocontrol activities of the selected strains. Moreover, P39 displays mycophagy, consuming fungal mycelia and transforming them into bacterial biomass, particularly in nutrient-poor media supplemented with fungal mycelia. The genome harbored chitin and N-acetylglucosamine utilization genes, suggesting a proposed pathway for the utilization of fungal cells as a nutrient source. Microscopic observations further supported the ability of this strain to rupture and damage fungal hyphae, depriving them of their cellular constituents. This study successfully demonstrated the implementation of a taxonomy-guided approach for the selection of bacterial strains for biocontrol. These findings contribute to our understanding of biocontrol strategies, bacteria-fungi interactions, and the identification of Paraburkholderia busanensis sp. nov. as a potential candidate for the biocontrol of pepper anthracnose. Additionally, this strain serves as a valuable resource for antifungal compounds and volatiles, and for the study of bacteria-fungal interactions and mycophagy. IMPORTANCE Traditional control methods for postharvest diseases rely on fungicides, which cause human health and environmental concerns. This study introduces a taxonomy-guided strategy for selecting biocontrol agents. By focusing on Paraburkholderia group, which harbors diverse plant-beneficial strains, the inadvertent selection of harmful strains was circumvented, thereby obviating the need for laborious in vitro screening assays. A highly promising candidate, strain P39, has been identified, exhibiting remarkable biocontrol activity against Colletotrichum scovillei . Through comprehensive genomic, physiological, and biochemical analyses, P39 was characterized as a novel species within the Paraburkholderia genus and designated Paraburkholderia busanensis . Moreover, these findings deepen our understanding of bacterial-fungal interactions, as they elucidate a potential pathway for the utilization of fungal chitin, thereby enhancing our understanding of bacterial mycophagy. P. busanensis is a promising source of antifungal volatiles and putative novel secondary metabolites.

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“…This research con rmed the potential of eco-friendly microbial fungicides based on streptomyces as alternatives to synthetic fungicides associated with various environmental and health issues. Several microbial fungicides based on Streptomyces strains are commercially available, such as AGBIO's MYCOSTOP based on S. griseoviridis K61 producing the polyene macrolide candicidin, and NOVOZYMES' ACTINOVATR based on S. lydicus WYEC108 producing the polyene macrolide natamycin [30] . Furthermore, ongoing research continue to con rm the potential of Streptomyces isolates as putative bio-fungicides.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Park,

Kang,

Choi

et al. 2023

Preprint

View full text Add to dashboard Cite

Soil microorganisms with diverse bioactive compounds such as Streptomyces are appreciated as valuable resources for the discovery of eco-friendly fungicides. This study isolated a novel Streptomyces from soil samples collected in the organic green tea fields in South Korea. The isolation process involved antifungal activity screening around 2400 culture extracts, revealing a strain designated as S. collinus Inha504 with remarkable antifungal activity against diverse phytopathogenic fungi. S. collinus Inha504 inhibited seven phytopathogenic fungi, including Fusarium oxysporum and Aspergillus niger, in bioassays and showed a control effect against F. oxysporum infected red pepper, strawberry, and tomato in the in vivo pot test. Genome mining of S. collinus Inha504 revealed the presence of the biosynthetic gene cluster (BGC) in the chromosome encoding a polyene macrolide which is highly homologous to the lucensomycin (LCM), a compound known for effective in crop disease control. Through genetic confirmation and bioassays, the antifungal activity of S. collinus Inha504 was attributed to the presence of LCM BGC in the chromosome. These results could serve as an effective strategy to select novel Streptomyces strains with valuable biological activity through bioassay-based screening and identify biosynthetic gene clusters responsible for the metabolites using genome mining approach.

show abstract

Potential of Streptomyces sp. strain AGS-58 in controlling anthracnose-causing Colletotrichum siamense from post-harvest mango fruits

Cited by 10 publications

References 54 publications

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

Taxonomy-guided selection of Paraburkholderia busanensis sp. nov.: a versatile biocontrol agent with mycophagy against Colletotrichum scovillei causing pepper anthracnose

Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster

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