Potential of Vasoprotectives to Inhibit Non-Enzymatic Protein Glycation, and Reactive Carbonyl and Oxygen Species Uptake

Bednarska, Katarzyna; Fecka, Izabela

doi:10.3390/ijms221810026

Cited by 20 publications

(32 citation statements)

References 70 publications

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“…An analogous set of peppermint polyphenols was used as in the glycation inhibition assay. The MGO-trapping ability of other flavones and flavanones such as vitexin (apigenin-8- C -β-glucoside), isovitexin (apigenin-8- C -β-glucoside), hesperidin, hesperetin, diosmin and diosmetin have been reported by our group previously [ 5 , 39 ]. Mono-adducts with MGO have been noted for all flavonoid aglycones, both flavones and flavanones.…”

Section: Resultsmentioning

confidence: 68%

“…The anti-AGE effect of polyphenols is related to their protective action against chemical modifications of substrates involved in the glycation reaction, such as binding to groups involved in the initiation of glycation or inhibiting the oxidation of simple sugars and ketoamines (Amadori products). A key component of this effect is the ability to capture glycation- or oxidation-initiating factors (RCS, ROS) [ 21 , 22 , 23 , 39 ]. Anti-MGO potential of luteolin-7- O -rutinoside, luteolin-7- O -β-glucuronoside, luteolin-7- O -β-glucoside and eriodictyol-7- O -rutinosides with regard to aglycones was lower, at 35–62% of their activity.…”

Section: Resultsmentioning

confidence: 99%

“…Several synthetic medicines and plant polyphenols are known to have the capacity for MGO scavenging [ 5 , 39 , 40 , 41 ]. However, only metformin is of practical therapeutic use in patients with type 2 diabetes and insulin resistance.…”

Section: Resultsmentioning

confidence: 99%

“…The presence of these new compounds in the reaction mixture is confirmed using chromatographic methods coupled to a mass detector (MS). Mono- and di-adducts of MGO with polyphenols are characterized by a pseudomolecular ion with an exact mass of 72.02 Da and 144.04 Da higher than the precursor ion, respectively [ 21 , 39 , 40 , 42 ].…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf (Mentha × piperita L.) and Its Polyphenols

2023

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The most significant reactive α-dicarbonyl RCS involved in the pathomechanism of glycation and related diseases is methylglyoxal (MGO). Hyperglycemia promotes the generation of MGO and leads to the formation of advanced glycation end products (AGEs). Therefore, MGO trapping and glycation inhibition appear to be important therapeutic targets in prediabetes, diabetes, and in the early prevention of hyperglycemic complications. Peppermint leaf is commonly used as herbal tea, rich in polyphenols. Eriocitrin, its predominant component, in a double-blind, randomized controlled study reversed the prediabetic condition in patients. However, the antiglycation activity of this plant material and its polyphenols has not been characterized to date. Therefore, the aim of this study was to evaluate the ability of a peppermint leaf dry extract and its polyphenols to inhibit non-enzymatic protein glycation in a model with bovine serum albumin (BSA) and MGO as a glycation agent. Peppermint polyphenols were also evaluated for their potential to trap MGO in vitro, and the resulting adducts were analyzed by UHPLC-ESI-MS. To relate chemical composition to glycation inhibitory activity, the obtained peppermint extract was subjected to qualitative and quantitative analysis. The capability of peppermint leaf polyphenols to inhibit glycation (27.3–77.2%) and form adducts with MGO was confirmed. In the case of flavone aglycones, mono- and di-adducts with MGO were observed, while eriodictyol and eriocitrin effectively produced only mono-adducts. Rosmarinic acid and luteolin-7-O-glycosides did not reveal this action. IC50 of the peppermint leaf dry extract was calculated at 2 mg/mL, equivalent to a concentration of 1.8 μM/mL of polyphenols, including ~1.4 μM/mL of flavonoids and ~0.4 μM/mL of phenolic acids. The contribution of the four major components to the anti-AGE activity of the extract was estimated at 86%, including eriocitrin 35.4%, rosmarinic acid 25.6%, luteolin-7-O-rutinoside 16.9%, luteolin-7-O-β-glucuronoside 8.1%, and others 14%. The effect of peppermint dry extract and polyphenols in inhibiting MGO-induced glycation in vitro was comparable to that of metformin used as a positive control.

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Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf (Mentha × piperita L.) and Its Polyphenols

2023

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“…The final step was to identify the adducts formed as a result of trapping and propose the potential structures formed, as well as to demonstrate how structural differences in flavonoid compounds affect trapping ability. Our previous research included the study of flavonoid O -glycosides (e.g., rutin, isoquercitrin, and hyperoside) in this regard, while in this work we focused on C -glycosidic compounds [ 47 , 48 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

Aspalathin and Other Rooibos Flavonoids Trapped α-Dicarbonyls and Inhibited Formation of Advanced Glycation End Products In Vitro

Bednarska

Fecka

2022

IJMS

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The excessive dietary intake of simple sugars and abnormal metabolism in certain diseases contribute to the increased production of α-dicarbonyls (α-DCs), such as methylglyoxal (MGO) and glyoxal (GO), the main precursors of the formation of advanced glycation end products (AGEs). AGEs play a vital role, for example, in the development of cardiovascular diseases and diabetes. Aspalathus linearis (Burman f.) R. Dahlgren (known as rooibos tea) exhibits a wide range of activities beneficial for cardio-metabolic health. Thus, the present study aims to investigate unfermented and fermented rooibos extracts and their constituents for the ability to trap MGO and GO. The individual compounds identified in extracts were tested for the capability to inhibit AGEs (with MGO or GO as a glycation agent). Ultra-high-performance liquid chromatography coupled with an electrospray ionization mass spectrometer (UHPLC–ESI–MS) was used to investigate α-DCs’ trapping capacities. To evaluate the antiglycation activity, fluorescence measurement was used. The extract from the unfermented rooibos showed a higher ability to capture MGO/GO and inhibit AGE formation than did the extract from fermented rooibos, and this effect was attributed to a higher content of dihydrochalcones. The compounds detected in the extracts, such as aspalathin, nothofagin, vitexin, isovitexin, and eriodictyol, as well as structurally related phloretin and phloroglucinol (formed by the biotransformation of certain flavonoids), trapped MGO, and some also trapped GO. AGE formation was inhibited the most by isovitexin. However, it was the high content of aspalathin and its higher efficiency than that of metformin that determined the antiglycation and trapping properties of green rooibos. Therefore, A. linearis, in addition to other health benefits, could potentially be used as an α-DC trapping agent and AGE inhibitor.

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AGE/Non-AGE Glycation: An Important Event in Rheumatoid Arthritis Pathophysiology

Monu,

Agnihotri,

Biswas

2021

Inflammation

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Rheumatoid arthritis (RA) is a chronic inflammatory, autoimmune disease that gradually affects the synovial membrane and joints. Many intrinsic and/or extrinsic factors are crucial in making RA pathology challenging throughout the disease. Substantial enzymatic or non-enzymatic modification of proteins driving inflammation has gained a lot of interest in recent years. Endogenously modified glycated protein influences disease development linked with AGEs/non-AGEs and is reported as a disease marker. In this review, we summarized current knowledge of the differential abundance of glycated proteins by compiling and analyzing a variety of AGE and non-AGE ligands that bind with RAGE to activate multi-faceted inflammatory and oxidative stress pathways that are pathobiologically associated with RA-fibroblast-like synoviocytes (RA-FLS). It is critical to comprehend the connection between oxidative stress and inflammation generation, mediated by glycated protein, which may bind to the receptor RAGE, activate downstream pathways, and impart immunogenicity in RA. It is worth noting that AGEs and non-AGEs ligands play a variety of functions, and their functionality is likely to be more reliant on pathogenic states and severity that may serve as biomarkers for RA. Screening and monitoring of these differentially glycated proteins, as well as their stability in circulation, in combination with established pre-clinical characteristics, may aid or predict the onset of RA.KEY WORDS: rheumatoid arthritis (RA); rheumatoid factors (RF); anti-citrullinated peptide antibodies (ACPAs); advanced glycation end-products (AGEs); glyoxal (GO); methylglyoxal (MGO); N-carboxymethyl lysine (CML); reactive oxygen species (ROS); interleukin (IL).

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Potential of Vasoprotectives to Inhibit Non-Enzymatic Protein Glycation, and Reactive Carbonyl and Oxygen Species Uptake

Cited by 20 publications

References 70 publications

In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf (Mentha × piperita L.) and Its Polyphenols

In Vitro Antiglycation and Methylglyoxal Trapping Effect of Peppermint Leaf (Mentha × piperita L.) and Its Polyphenols

Aspalathin and Other Rooibos Flavonoids Trapped α-Dicarbonyls and Inhibited Formation of Advanced Glycation End Products In Vitro

AGE/Non-AGE Glycation: An Important Event in Rheumatoid Arthritis Pathophysiology

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