Potential of yeast secretory vesicles in biodelivery systems

Kutralam-Muniasamy, Gurusamy; Flores-Cotera, Luis B.; Pérez-Guevara, Fermín

doi:10.1016/j.drudis.2015.03.014

Cited by 6 publications

(4 citation statements)

References 59 publications

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“…The results of the CCK-8 assay showed that X33@sEVs, FaeA@sEVs and FaeA@sEVs-FA all had a wide range of safe concentrations, and could promote the growth of HEK 293T cells within a certain concentration range ( Figure 6B ). sEV is characterized as nano-sized and biocompatible characteristics ( Kutralam-Muniasamy et al, 2015 ; Henriques-Antunes et al, 2019 ), which not only improve the stability of FA but also enhance the uptake of FA by cells. Notably, in the FA concentration range of 0.1–1.0 μM, both free FA and FaeA@sEVs-FA showed the promotion of cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

Engineered small extracellular vesicles as a versatile platform to efficiently load ferulic acid via an “esterase-responsive active loading” strategy

Man

Xing

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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As nano-drug carriers, small extracellular vesicles (sEVs) have shown unique advantages, but their drug loading and encapsulation efficiency are far from being satisfied, especially for the loading of hydrophilic small-molecule drugs. Inspired by the strategies of active loading of liposomal nanomedicines, pre-drug design and immobilization enzyme, here we developed a new platform, named “Esterase-responsive Active Loading” (EAL), for the efficient and stable drug encapsulation of sEVs. Widely used ferulic acid ester derivatives were chosen as prodrugs based on the EAL of engineered sEVs to establish a continuous transmembrane ion gradient for achieving efficient loading of active molecule ferulic acid into sEVs. The EAL showed that the drug loading and encapsulation efficiency were around 6-fold and 5-fold higher than passive loading, respectively. Moreover, characterization by nano-flow cytometry and Malvern particle size analyzer showed that differential ultracentrifugation combined with multiple types of membrane filtration methods can achieve large-scale and high-quality production of sEVs. Finally, extracellular and intracellular assessments further confirmed the superior performance of the EAL-prepared sEVs-loaded ferulic acid preparation in terms of slow release and low toxicity. Taken together, these findings will provide an instructive insight into the development of sEV-based delivery systems.

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Section: Resultsmentioning

confidence: 99%

Engineered small extracellular vesicles as a versatile platform to efficiently load ferulic acid via an “esterase-responsive active loading” strategy

Man

Xing

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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“…However, a significant but minor Al transport activity was also observed for the yeast line carrying OsNIP1;2 in the presence of Al 3+ ions ( Figure 6 and Supplementary Figure 5 ). As yeast cells secrete cellular ligands into the growth media ( Kutralam-Muniasamy et al, 2015 ), the external ligands and Al 3+ could form Al-ligand complexes in the uptake medium, which could be taken up by OsNIP1;2. Therefore, the Al taken up by OsNIP1;2 in the Al 3+ condition ( Figure 6 and Supplementary Figure 5 ) could be in the form of Al-ligands but not as Al 3+ ions.…”

Section: Discussionmentioning

confidence: 99%

The plasma membrane-localized OsNIP1;2 mediates internal aluminum detoxification in rice

Wang

Shen

et al. 2022

Front. Plant Sci.

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Aluminum (Al) toxicity significantly restricts crop production on acidic soils. Although rice is highly resistant to Al stress, the underlying resistant mechanisms are not fully understood. Here, we characterized the function of OsNIP1;2, a plasma membrane-localized nodulin 26-like intrinsic protein (NIP) in rice. Aluminum stress specifically and quickly induced OsNIP1;2 expression in the root. Functional mutations of OsNIP1;2 in two independent rice lines led to significantly enhanced sensitivity to Al but not other metals. Moreover, the Osnip1;2 mutants had considerably more Al accumulated in the root cell wall but less in the cytosol than the wild-type rice. In addition, compared with the wild-type rice plants, the Osnip1;2 mutants contained more Al in the root but less in the shoot. When expressed in yeast, OsNIP1;2 led to enhanced Al accumulation in the cells and enhanced sensitivity to Al stress, suggesting that OsNIP1;2 facilitated Al uptake in yeast. These results suggest that OsNIP1;2 confers internal Al detoxification via taking out the root cell wall’s Al, sequestering it to the root cell’s vacuole, and re-distributing it to the above-ground tissues.

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“…All living organisms, ranging from bacteria to fungi and from germ line to neurons in metazoans, release EVs [ 16 , 17 , 18 ]. In-depth studies have also been carried out on yeast, which is an excellent model system to study the trafficking of different types of EVs [ 19 ], such as post-Golgi vesicles (PGVs), and their application in the biomedical field [ 20 ]. Likewise, C. elegans early embryos and Drosophila secretory glands are tissues where the release of EVs is predominant; for this reason, they are crucial models for the comprehension of the generation, behavioral and functional roles of EVs [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Salting-Out Approach Is Worthy of Comparison with Ultracentrifugation for Extracellular Vesicle Isolation from Tumor and Healthy Models

et al. 2021

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The role of extracellular vesicles (EVs) has been completely re-evaluated in the recent decades, and EVs are currently considered to be among the main players in intercellular communication. Beyond their functional aspects, there is strong interest in the development of faster and less expensive isolation protocols that are as reliable for post-isolation characterisations as already-established methods. Therefore, the identification of easy and accessible EV isolation techniques with a low price/performance ratio is of paramount importance. We isolated EVs from a wide spectrum of samples of biological and clinical interest by choosing two isolation techniques, based on their wide use and affordability: ultracentrifugation and salting-out. We collected EVs from human cancer and healthy cell culture media, yeast, bacteria and Drosophila culture media and human fluids (plasma, urine and saliva). The size distribution and concentration of EVs were measured by nanoparticle tracking analysis and dynamic light scattering, and protein depletion was measured by a colorimetric nanoplasmonic assay. Finally, the EVs were characterised by flow cytometry. Our results showed that the salting-out method had a good efficiency in EV separation and was more efficient in protein depletion than ultracentrifugation. Thus, salting-out may represent a good alternative to ultracentrifugation.

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Potential of yeast secretory vesicles in biodelivery systems

Cited by 6 publications

References 59 publications

Engineered small extracellular vesicles as a versatile platform to efficiently load ferulic acid via an “esterase-responsive active loading” strategy

Engineered small extracellular vesicles as a versatile platform to efficiently load ferulic acid via an “esterase-responsive active loading” strategy

The plasma membrane-localized OsNIP1;2 mediates internal aluminum detoxification in rice

Salting-Out Approach Is Worthy of Comparison with Ultracentrifugation for Extracellular Vesicle Isolation from Tumor and Healthy Models

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